A Plasmid-Borne Gene Cluster Flanked by Two Restriction-Modification Systems Enables an Arctic Strain of Psychrobacter sp. to Decompose SDS
The cold-adapted Psychrobacter sp. strain DAB_AL62B, isolated from ornithogenic deposits on the Arctic island of Spitsbergen, harbors a 34.5 kb plasmid, pP62BP1, which carries a genetic SLF module predicted to enable the host bacterium to metabolize alkyl sulfates including sodium dodecyl sulfate (S...
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2023
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ftdoajarticles:oai:doaj.org/article:e6e45bea9dc7416f9e6402488494166c 2024-02-11T10:00:56+01:00 A Plasmid-Borne Gene Cluster Flanked by Two Restriction-Modification Systems Enables an Arctic Strain of Psychrobacter sp. to Decompose SDS Robert Lasek Ignacy Piszczek Monika Krolikowski Adrian Sówka Dariusz Bartosik 2023-12-01T00:00:00Z https://doi.org/10.3390/ijms25010551 https://doaj.org/article/e6e45bea9dc7416f9e6402488494166c EN eng MDPI AG https://www.mdpi.com/1422-0067/25/1/551 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms25010551 1422-0067 1661-6596 https://doaj.org/article/e6e45bea9dc7416f9e6402488494166c International Journal of Molecular Sciences, Vol 25, Iss 1, p 551 (2023) Psychrobacter psychrophile SDS degradation alkyl sulfatase restriction–modification system Biology (General) QH301-705.5 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/ijms25010551 2024-01-14T01:39:03Z The cold-adapted Psychrobacter sp. strain DAB_AL62B, isolated from ornithogenic deposits on the Arctic island of Spitsbergen, harbors a 34.5 kb plasmid, pP62BP1, which carries a genetic SLF module predicted to enable the host bacterium to metabolize alkyl sulfates including sodium dodecyl sulfate (SDS), a common anionic surfactant. In this work, we experimentally confirmed that the pP62BP1-harboring strain is capable of SDS degradation. The slfCHSL genes were shown to form an operon whose main promoter, P slfC , is negatively regulated by the product of the slfR gene in the absence of potential substrates. We showed that lauryl aldehyde acts as an inducer of the operon. The analysis of the draft genome sequence of the DAB_AL62B strain revealed that the crucial enzyme of the SDS degradation pathway—an alkyl sulfatase—is encoded only within the plasmid. The SLF module is flanked by two restriction–modification systems, which were shown to exhibit the same sequence specificity. We hypothesize that the maintenance of pP62BP1 may be dependent on this unique genetic organization. Article in Journal/Newspaper Arctic Spitsbergen Directory of Open Access Journals: DOAJ Articles Arctic Arctic Island ENVELOPE(-74.766,-74.766,62.234,62.234) International Journal of Molecular Sciences 25 1 551 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Psychrobacter psychrophile SDS degradation alkyl sulfatase restriction–modification system Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
Psychrobacter psychrophile SDS degradation alkyl sulfatase restriction–modification system Biology (General) QH301-705.5 Chemistry QD1-999 Robert Lasek Ignacy Piszczek Monika Krolikowski Adrian Sówka Dariusz Bartosik A Plasmid-Borne Gene Cluster Flanked by Two Restriction-Modification Systems Enables an Arctic Strain of Psychrobacter sp. to Decompose SDS |
topic_facet |
Psychrobacter psychrophile SDS degradation alkyl sulfatase restriction–modification system Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
The cold-adapted Psychrobacter sp. strain DAB_AL62B, isolated from ornithogenic deposits on the Arctic island of Spitsbergen, harbors a 34.5 kb plasmid, pP62BP1, which carries a genetic SLF module predicted to enable the host bacterium to metabolize alkyl sulfates including sodium dodecyl sulfate (SDS), a common anionic surfactant. In this work, we experimentally confirmed that the pP62BP1-harboring strain is capable of SDS degradation. The slfCHSL genes were shown to form an operon whose main promoter, P slfC , is negatively regulated by the product of the slfR gene in the absence of potential substrates. We showed that lauryl aldehyde acts as an inducer of the operon. The analysis of the draft genome sequence of the DAB_AL62B strain revealed that the crucial enzyme of the SDS degradation pathway—an alkyl sulfatase—is encoded only within the plasmid. The SLF module is flanked by two restriction–modification systems, which were shown to exhibit the same sequence specificity. We hypothesize that the maintenance of pP62BP1 may be dependent on this unique genetic organization. |
format |
Article in Journal/Newspaper |
author |
Robert Lasek Ignacy Piszczek Monika Krolikowski Adrian Sówka Dariusz Bartosik |
author_facet |
Robert Lasek Ignacy Piszczek Monika Krolikowski Adrian Sówka Dariusz Bartosik |
author_sort |
Robert Lasek |
title |
A Plasmid-Borne Gene Cluster Flanked by Two Restriction-Modification Systems Enables an Arctic Strain of Psychrobacter sp. to Decompose SDS |
title_short |
A Plasmid-Borne Gene Cluster Flanked by Two Restriction-Modification Systems Enables an Arctic Strain of Psychrobacter sp. to Decompose SDS |
title_full |
A Plasmid-Borne Gene Cluster Flanked by Two Restriction-Modification Systems Enables an Arctic Strain of Psychrobacter sp. to Decompose SDS |
title_fullStr |
A Plasmid-Borne Gene Cluster Flanked by Two Restriction-Modification Systems Enables an Arctic Strain of Psychrobacter sp. to Decompose SDS |
title_full_unstemmed |
A Plasmid-Borne Gene Cluster Flanked by Two Restriction-Modification Systems Enables an Arctic Strain of Psychrobacter sp. to Decompose SDS |
title_sort |
plasmid-borne gene cluster flanked by two restriction-modification systems enables an arctic strain of psychrobacter sp. to decompose sds |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms25010551 https://doaj.org/article/e6e45bea9dc7416f9e6402488494166c |
long_lat |
ENVELOPE(-74.766,-74.766,62.234,62.234) |
geographic |
Arctic Arctic Island |
geographic_facet |
Arctic Arctic Island |
genre |
Arctic Spitsbergen |
genre_facet |
Arctic Spitsbergen |
op_source |
International Journal of Molecular Sciences, Vol 25, Iss 1, p 551 (2023) |
op_relation |
https://www.mdpi.com/1422-0067/25/1/551 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms25010551 1422-0067 1661-6596 https://doaj.org/article/e6e45bea9dc7416f9e6402488494166c |
op_doi |
https://doi.org/10.3390/ijms25010551 |
container_title |
International Journal of Molecular Sciences |
container_volume |
25 |
container_issue |
1 |
container_start_page |
551 |
_version_ |
1790596660526579712 |