A Novel Cold-Adapted Leucine Dehydrogenase from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. ANT178
l-tert-leucine and its derivatives are useful as pharmaceutical active ingredients, in which leucine dehydrogenase (LeuDH) is the key enzyme in their enzymatic conversions. In the present study, a novel cold-adapted LeuDH, psleudh, was cloned from psychrotrophic bacteria Pseudoalteromonas sp. ANT178...
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ftdoajarticles:oai:doaj.org/article:e6b91c55ffb64eaead2387bb0aee4893 2023-05-15T13:44:19+02:00 A Novel Cold-Adapted Leucine Dehydrogenase from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. ANT178 Yatong Wang Yanhua Hou Yifan Wang Lu Zheng Xianlei Xu Kang Pan Rongqi Li Quanfu Wang 2018-10-01T00:00:00Z https://doi.org/10.3390/md16100359 https://doaj.org/article/e6b91c55ffb64eaead2387bb0aee4893 EN eng MDPI AG http://www.mdpi.com/1660-3397/16/10/359 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md16100359 https://doaj.org/article/e6b91c55ffb64eaead2387bb0aee4893 Marine Drugs, Vol 16, Iss 10, p 359 (2018) leucine dehydrogenase cold-adapted Antarctic bacterium sea-ice homology modeling Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.3390/md16100359 2022-12-31T00:17:18Z l-tert-leucine and its derivatives are useful as pharmaceutical active ingredients, in which leucine dehydrogenase (LeuDH) is the key enzyme in their enzymatic conversions. In the present study, a novel cold-adapted LeuDH, psleudh, was cloned from psychrotrophic bacteria Pseudoalteromonas sp. ANT178, which was isolated from Antarctic sea-ice. Bioinformatics analysis of the gene psleudh showed that the gene was 1209 bp in length and coded for a 42.6 kDa protein containing 402 amino acids. PsLeuDH had conserved Phe binding site and NAD+ binding site, and belonged to a member of the Glu/Leu/Phe/Val dehydrogenase family. Homology modeling analysis results suggested that PsLeuDH exhibited more glycine residues, reduced proline residues, and arginine residues, which might be responsible for its catalytic efficiency at low temperature. The recombinant PsLeuDH (rPsLeuDH) was purified a major band with the high specific activity of 275.13 U/mg using a Ni-NTA affinity chromatography. The optimum temperature and pH for rPsLeuDH activity were 30 °C and pH 9.0, respectively. Importantly, rPsLeuDH retained at least 40% of its maximum activity even at 0 °C. Moreover, the activity of rPsLeuDH was the highest in the presence of 2.0 M NaCl. Substrate specificity and kinetic studies of rPsLeuDH demonstrated that l-leucine was the most suitable substrate, and the catalytic activity at low temperatures was ensured by maintaining a high kcat value. The results of the current study would provide insight into Antarctic sea-ice bacterium LeuDH, and the unique properties of rPsLeuDH make it a promising candidate as a biocatalyst in medical and pharmaceutical industries. Article in Journal/Newspaper Antarc* Antarctic Sea ice Directory of Open Access Journals: DOAJ Articles Antarctic Marine Drugs 16 10 359 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
leucine dehydrogenase cold-adapted Antarctic bacterium sea-ice homology modeling Biology (General) QH301-705.5 |
spellingShingle |
leucine dehydrogenase cold-adapted Antarctic bacterium sea-ice homology modeling Biology (General) QH301-705.5 Yatong Wang Yanhua Hou Yifan Wang Lu Zheng Xianlei Xu Kang Pan Rongqi Li Quanfu Wang A Novel Cold-Adapted Leucine Dehydrogenase from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. ANT178 |
topic_facet |
leucine dehydrogenase cold-adapted Antarctic bacterium sea-ice homology modeling Biology (General) QH301-705.5 |
description |
l-tert-leucine and its derivatives are useful as pharmaceutical active ingredients, in which leucine dehydrogenase (LeuDH) is the key enzyme in their enzymatic conversions. In the present study, a novel cold-adapted LeuDH, psleudh, was cloned from psychrotrophic bacteria Pseudoalteromonas sp. ANT178, which was isolated from Antarctic sea-ice. Bioinformatics analysis of the gene psleudh showed that the gene was 1209 bp in length and coded for a 42.6 kDa protein containing 402 amino acids. PsLeuDH had conserved Phe binding site and NAD+ binding site, and belonged to a member of the Glu/Leu/Phe/Val dehydrogenase family. Homology modeling analysis results suggested that PsLeuDH exhibited more glycine residues, reduced proline residues, and arginine residues, which might be responsible for its catalytic efficiency at low temperature. The recombinant PsLeuDH (rPsLeuDH) was purified a major band with the high specific activity of 275.13 U/mg using a Ni-NTA affinity chromatography. The optimum temperature and pH for rPsLeuDH activity were 30 °C and pH 9.0, respectively. Importantly, rPsLeuDH retained at least 40% of its maximum activity even at 0 °C. Moreover, the activity of rPsLeuDH was the highest in the presence of 2.0 M NaCl. Substrate specificity and kinetic studies of rPsLeuDH demonstrated that l-leucine was the most suitable substrate, and the catalytic activity at low temperatures was ensured by maintaining a high kcat value. The results of the current study would provide insight into Antarctic sea-ice bacterium LeuDH, and the unique properties of rPsLeuDH make it a promising candidate as a biocatalyst in medical and pharmaceutical industries. |
format |
Article in Journal/Newspaper |
author |
Yatong Wang Yanhua Hou Yifan Wang Lu Zheng Xianlei Xu Kang Pan Rongqi Li Quanfu Wang |
author_facet |
Yatong Wang Yanhua Hou Yifan Wang Lu Zheng Xianlei Xu Kang Pan Rongqi Li Quanfu Wang |
author_sort |
Yatong Wang |
title |
A Novel Cold-Adapted Leucine Dehydrogenase from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. ANT178 |
title_short |
A Novel Cold-Adapted Leucine Dehydrogenase from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. ANT178 |
title_full |
A Novel Cold-Adapted Leucine Dehydrogenase from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. ANT178 |
title_fullStr |
A Novel Cold-Adapted Leucine Dehydrogenase from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. ANT178 |
title_full_unstemmed |
A Novel Cold-Adapted Leucine Dehydrogenase from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. ANT178 |
title_sort |
novel cold-adapted leucine dehydrogenase from antarctic sea-ice bacterium pseudoalteromonas sp. ant178 |
publisher |
MDPI AG |
publishDate |
2018 |
url |
https://doi.org/10.3390/md16100359 https://doaj.org/article/e6b91c55ffb64eaead2387bb0aee4893 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Sea ice |
genre_facet |
Antarc* Antarctic Sea ice |
op_source |
Marine Drugs, Vol 16, Iss 10, p 359 (2018) |
op_relation |
http://www.mdpi.com/1660-3397/16/10/359 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md16100359 https://doaj.org/article/e6b91c55ffb64eaead2387bb0aee4893 |
op_doi |
https://doi.org/10.3390/md16100359 |
container_title |
Marine Drugs |
container_volume |
16 |
container_issue |
10 |
container_start_page |
359 |
_version_ |
1766200153103925248 |