Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii
The tropical marine cyanobacterium Moorena bouillonii occupies a large geographic range across the Indian and Western Tropical Pacific Oceans and is a prolific producer of structurally unique and biologically active natural products. An ensemble of computational approaches, including the creation of...
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ftdoajarticles:oai:doaj.org/article:e68691c31f534a5ca9a3b1ea660216df 2023-05-15T17:53:49+02:00 Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii Christopher A. Leber C. Benjamin Naman Lena Keller Jehad Almaliti Eduardo J. E. Caro-Diaz Evgenia Glukhov Valsamma Joseph T. P. Sajeevan Andres Joshua Reyes Jason S. Biggs Te Li Ye Yuan Shan He Xiaojun Yan William H. Gerwick 2020-10-01T00:00:00Z https://doi.org/10.3390/md18100515 https://doaj.org/article/e68691c31f534a5ca9a3b1ea660216df EN eng MDPI AG https://www.mdpi.com/1660-3397/18/10/515 https://doaj.org/toc/1660-3397 doi:10.3390/md18100515 1660-3397 https://doaj.org/article/e68691c31f534a5ca9a3b1ea660216df Marine Drugs, Vol 18, Iss 515, p 515 (2020) Moorena bouillonii marine natural products chemogeography metabolomics Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.3390/md18100515 2022-12-30T20:01:51Z The tropical marine cyanobacterium Moorena bouillonii occupies a large geographic range across the Indian and Western Tropical Pacific Oceans and is a prolific producer of structurally unique and biologically active natural products. An ensemble of computational approaches, including the creation of the ORCA (Objective Relational Comparative Analysis) pipeline for flexible MS 1 feature detection and multivariate analyses, were used to analyze various M. bouillonii samples. The observed chemogeographic patterns suggested the production of regionally specific natural products by M. bouillonii . Analyzing the drivers of these chemogeographic patterns allowed for the identification, targeted isolation, and structure elucidation of a regionally specific natural product, doscadenamide A ( 1 ). Analyses of MS 2 fragmentation patterns further revealed this natural product to be part of an extensive family of herein annotated, proposed natural structural analogs (doscadenamides B–J, 2–10); the ensemble of structures reflect a combinatorial biosynthesis using nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) components. Compound 1 displayed synergistic in vitro cancer cell cytotoxicity when administered with lipopolysaccharide (LPS). These discoveries illustrate the utility in leveraging chemogeographic patterns for prioritizing natural product discovery efforts. Article in Journal/Newspaper Orca Directory of Open Access Journals: DOAJ Articles Indian Pacific Marine Drugs 18 10 515 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Moorena bouillonii marine natural products chemogeography metabolomics Biology (General) QH301-705.5 |
spellingShingle |
Moorena bouillonii marine natural products chemogeography metabolomics Biology (General) QH301-705.5 Christopher A. Leber C. Benjamin Naman Lena Keller Jehad Almaliti Eduardo J. E. Caro-Diaz Evgenia Glukhov Valsamma Joseph T. P. Sajeevan Andres Joshua Reyes Jason S. Biggs Te Li Ye Yuan Shan He Xiaojun Yan William H. Gerwick Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii |
topic_facet |
Moorena bouillonii marine natural products chemogeography metabolomics Biology (General) QH301-705.5 |
description |
The tropical marine cyanobacterium Moorena bouillonii occupies a large geographic range across the Indian and Western Tropical Pacific Oceans and is a prolific producer of structurally unique and biologically active natural products. An ensemble of computational approaches, including the creation of the ORCA (Objective Relational Comparative Analysis) pipeline for flexible MS 1 feature detection and multivariate analyses, were used to analyze various M. bouillonii samples. The observed chemogeographic patterns suggested the production of regionally specific natural products by M. bouillonii . Analyzing the drivers of these chemogeographic patterns allowed for the identification, targeted isolation, and structure elucidation of a regionally specific natural product, doscadenamide A ( 1 ). Analyses of MS 2 fragmentation patterns further revealed this natural product to be part of an extensive family of herein annotated, proposed natural structural analogs (doscadenamides B–J, 2–10); the ensemble of structures reflect a combinatorial biosynthesis using nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) components. Compound 1 displayed synergistic in vitro cancer cell cytotoxicity when administered with lipopolysaccharide (LPS). These discoveries illustrate the utility in leveraging chemogeographic patterns for prioritizing natural product discovery efforts. |
format |
Article in Journal/Newspaper |
author |
Christopher A. Leber C. Benjamin Naman Lena Keller Jehad Almaliti Eduardo J. E. Caro-Diaz Evgenia Glukhov Valsamma Joseph T. P. Sajeevan Andres Joshua Reyes Jason S. Biggs Te Li Ye Yuan Shan He Xiaojun Yan William H. Gerwick |
author_facet |
Christopher A. Leber C. Benjamin Naman Lena Keller Jehad Almaliti Eduardo J. E. Caro-Diaz Evgenia Glukhov Valsamma Joseph T. P. Sajeevan Andres Joshua Reyes Jason S. Biggs Te Li Ye Yuan Shan He Xiaojun Yan William H. Gerwick |
author_sort |
Christopher A. Leber |
title |
Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii |
title_short |
Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii |
title_full |
Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii |
title_fullStr |
Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii |
title_full_unstemmed |
Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii |
title_sort |
applying a chemogeographic strategy for natural product discovery from the marine cyanobacterium moorena bouillonii |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/md18100515 https://doaj.org/article/e68691c31f534a5ca9a3b1ea660216df |
geographic |
Indian Pacific |
geographic_facet |
Indian Pacific |
genre |
Orca |
genre_facet |
Orca |
op_source |
Marine Drugs, Vol 18, Iss 515, p 515 (2020) |
op_relation |
https://www.mdpi.com/1660-3397/18/10/515 https://doaj.org/toc/1660-3397 doi:10.3390/md18100515 1660-3397 https://doaj.org/article/e68691c31f534a5ca9a3b1ea660216df |
op_doi |
https://doi.org/10.3390/md18100515 |
container_title |
Marine Drugs |
container_volume |
18 |
container_issue |
10 |
container_start_page |
515 |
_version_ |
1766161525769240576 |