Augmented plasma microparticles during acute Plasmodium vivax infection
Abstract Background In the last few years, the study of microparticles (MPs) - submicron vesicles released from cells upon activation or apoptosis - has gained growing interest in the field of inflammation and in infectious diseases. Their role in the human malaria parasite Plasmodium vivax remains...
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ftdoajarticles:oai:doaj.org/article:e6677ff6d0d0475eabc9187a30d6a04b 2023-05-15T15:13:47+02:00 Augmented plasma microparticles during acute Plasmodium vivax infection Brito Cristiana F Fontes Cor J de Paula Sálua CO Filho Agnaldo LS Teixeira-Carvalho Andréa Franklin Bernardo S Campos Fernanda MF Carvalho Luzia H 2010-11-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-327 https://doaj.org/article/e6677ff6d0d0475eabc9187a30d6a04b EN eng BMC http://www.malariajournal.com/content/9/1/327 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-327 1475-2875 https://doaj.org/article/e6677ff6d0d0475eabc9187a30d6a04b Malaria Journal, Vol 9, Iss 1, p 327 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-327 2022-12-31T08:46:14Z Abstract Background In the last few years, the study of microparticles (MPs) - submicron vesicles released from cells upon activation or apoptosis - has gained growing interest in the field of inflammation and in infectious diseases. Their role in the human malaria parasite Plasmodium vivax remains unexplored. Because acute vivax malaria has been related to pro-inflammatory responses, the main hypothesis investigated in this study was that Plasmodium vivax infection is associated with elevated levels of circulating MPs, which may play a role during acute disease in non-immune patients. Methods Plasma MPs were analysed among thirty-seven uncomplicated P. vivax infections from an area of unstable malaria transmission in the Brazilian Amazon. The MP phenotype was analysed by flow cytometry using the classical MP marker, annexin, and fluorochrome-labeled monoclonal antibodies against specific cell surface markers. The frequencies of plasma MPs in P. vivax patients (n = 37) were further compared to malaria-unexposed controls (n = 15) and ovarian carcinoma patients (n = 12), a known MPs-inducing disease non-related to malaria. Results The frequencies of plasma circulating MPs were markedly increased in P. vivax patients, as compared to healthy age-matched malaria-unexposed controls. Although platelets, erythrocytes and leukocytes were the main cellular sources of MPs during vivax malaria, platelet derived-MPs (PMPs) increased in a linear fashion with the presence of fever at the time of blood collection (β = 0.06, p < 0.0001) and length of acute symptoms (β = 0.36, p < 0.0001). Finally, the results suggest that plasma levels of PMPs diminish as patient experience more episodes of clinical malaria (β = 0.07, p < 0.003). Conclusions Abundant circulating MPs are present during acute P. vivax infection, and platelet derived-MPs may play a role on the acute inflammatory symptoms of malaria vivax. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 9 1 327 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Brito Cristiana F Fontes Cor J de Paula Sálua CO Filho Agnaldo LS Teixeira-Carvalho Andréa Franklin Bernardo S Campos Fernanda MF Carvalho Luzia H Augmented plasma microparticles during acute Plasmodium vivax infection |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background In the last few years, the study of microparticles (MPs) - submicron vesicles released from cells upon activation or apoptosis - has gained growing interest in the field of inflammation and in infectious diseases. Their role in the human malaria parasite Plasmodium vivax remains unexplored. Because acute vivax malaria has been related to pro-inflammatory responses, the main hypothesis investigated in this study was that Plasmodium vivax infection is associated with elevated levels of circulating MPs, which may play a role during acute disease in non-immune patients. Methods Plasma MPs were analysed among thirty-seven uncomplicated P. vivax infections from an area of unstable malaria transmission in the Brazilian Amazon. The MP phenotype was analysed by flow cytometry using the classical MP marker, annexin, and fluorochrome-labeled monoclonal antibodies against specific cell surface markers. The frequencies of plasma MPs in P. vivax patients (n = 37) were further compared to malaria-unexposed controls (n = 15) and ovarian carcinoma patients (n = 12), a known MPs-inducing disease non-related to malaria. Results The frequencies of plasma circulating MPs were markedly increased in P. vivax patients, as compared to healthy age-matched malaria-unexposed controls. Although platelets, erythrocytes and leukocytes were the main cellular sources of MPs during vivax malaria, platelet derived-MPs (PMPs) increased in a linear fashion with the presence of fever at the time of blood collection (β = 0.06, p < 0.0001) and length of acute symptoms (β = 0.36, p < 0.0001). Finally, the results suggest that plasma levels of PMPs diminish as patient experience more episodes of clinical malaria (β = 0.07, p < 0.003). Conclusions Abundant circulating MPs are present during acute P. vivax infection, and platelet derived-MPs may play a role on the acute inflammatory symptoms of malaria vivax. |
format |
Article in Journal/Newspaper |
author |
Brito Cristiana F Fontes Cor J de Paula Sálua CO Filho Agnaldo LS Teixeira-Carvalho Andréa Franklin Bernardo S Campos Fernanda MF Carvalho Luzia H |
author_facet |
Brito Cristiana F Fontes Cor J de Paula Sálua CO Filho Agnaldo LS Teixeira-Carvalho Andréa Franklin Bernardo S Campos Fernanda MF Carvalho Luzia H |
author_sort |
Brito Cristiana F |
title |
Augmented plasma microparticles during acute Plasmodium vivax infection |
title_short |
Augmented plasma microparticles during acute Plasmodium vivax infection |
title_full |
Augmented plasma microparticles during acute Plasmodium vivax infection |
title_fullStr |
Augmented plasma microparticles during acute Plasmodium vivax infection |
title_full_unstemmed |
Augmented plasma microparticles during acute Plasmodium vivax infection |
title_sort |
augmented plasma microparticles during acute plasmodium vivax infection |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-327 https://doaj.org/article/e6677ff6d0d0475eabc9187a30d6a04b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 327 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/327 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-327 1475-2875 https://doaj.org/article/e6677ff6d0d0475eabc9187a30d6a04b |
op_doi |
https://doi.org/10.1186/1475-2875-9-327 |
container_title |
Malaria Journal |
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9 |
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1 |
container_start_page |
327 |
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