HPLC-DAD analysis and antifungal effect of Hyptis martiusii Benth (Lamiaceae) against Candida strains
Objective: To evaluate the anti Candida activity of Hyptis martiusii decoction and its major compound, caffeic acid alone or in the presence of fluconazole, as well as their cytotoxic effect. Methods: The decoction was characterized using high performance liquid chromatography coupled with diode arr...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2019
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Online Access: | https://doi.org/10.4103/2221-1691.254606 https://doaj.org/article/e6502fff0dd043bcb75358f623d89f94 |
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ftdoajarticles:oai:doaj.org/article:e6502fff0dd043bcb75358f623d89f94 2023-05-15T15:11:26+02:00 HPLC-DAD analysis and antifungal effect of Hyptis martiusii Benth (Lamiaceae) against Candida strains Janaína Esmeraldo Rocha Henrique Douglas Melo Coutinho Cícero Roberto Nascimento Saraiva Antonia Thassya Lucas dos Santos Antônio Judson Targino Machado João Tavares Calixto Júnior Irwin Rose Alencar Menezes José Galberto Martins da Costa Jaime Ribeiro-Filho Aracelio Viana Colares 2019-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.254606 https://doaj.org/article/e6502fff0dd043bcb75358f623d89f94 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=3;spage=123;epage=128;aulast=Rocha https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.254606 https://doaj.org/article/e6502fff0dd043bcb75358f623d89f94 Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 3, Pp 123-128 (2019) hyptis martiusii caffeic acid candida albicans candida tropicalis antifungal properties Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.4103/2221-1691.254606 2022-12-31T10:11:02Z Objective: To evaluate the anti Candida activity of Hyptis martiusii decoction and its major compound, caffeic acid alone or in the presence of fluconazole, as well as their cytotoxic effect. Methods: The decoction was characterized using high performance liquid chromatography coupled with diode array detector. For the antifungal activity, the minimum inhibitory concentration (MIC) and the potential effect of the decoction with the fluconazole were evaluated by microdilution method using 96-well microtiter trays. The osmotic fragility test was performed using erythrocytes under saline stress. All tests were performed in triplicate. Results: The chemical characterization of the decoction was performed by high performance liquid chromatography and revealed the presence of seven compounds, including caffeic acid as major constituent. The antifungal tests demonstrated that both decoction (DHm) and caffeic acid obtained from Hyptis martiusii presented MIC and MFC ≥ 4096 μg/mL against Candida albicans and Candida tropicalis strains. However, in the presence of fluconazole, DHm and caffeic acid presented IC50 of 2.60 and 2.53 μg/mL respectively, demonstrating significant synergistic effects against Candida strains. The modulator activity of DHm might be due to the presence of caffeic acid. Moreover, DHm and caffeic acid did not cause significant hemolytic effects, indicating that they present low cytotoxicity. Conclusions: These data indicate that DHm potentiates the activity of the fluconazole, without enhancement of the toxicity, encouraging further toxicological, pharmacological and phytochemical studies to provide consistent evidence of the potential of this plant to be used in drug development. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 9 3 123 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
hyptis martiusii caffeic acid candida albicans candida tropicalis antifungal properties Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
hyptis martiusii caffeic acid candida albicans candida tropicalis antifungal properties Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Janaína Esmeraldo Rocha Henrique Douglas Melo Coutinho Cícero Roberto Nascimento Saraiva Antonia Thassya Lucas dos Santos Antônio Judson Targino Machado João Tavares Calixto Júnior Irwin Rose Alencar Menezes José Galberto Martins da Costa Jaime Ribeiro-Filho Aracelio Viana Colares HPLC-DAD analysis and antifungal effect of Hyptis martiusii Benth (Lamiaceae) against Candida strains |
topic_facet |
hyptis martiusii caffeic acid candida albicans candida tropicalis antifungal properties Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the anti Candida activity of Hyptis martiusii decoction and its major compound, caffeic acid alone or in the presence of fluconazole, as well as their cytotoxic effect. Methods: The decoction was characterized using high performance liquid chromatography coupled with diode array detector. For the antifungal activity, the minimum inhibitory concentration (MIC) and the potential effect of the decoction with the fluconazole were evaluated by microdilution method using 96-well microtiter trays. The osmotic fragility test was performed using erythrocytes under saline stress. All tests were performed in triplicate. Results: The chemical characterization of the decoction was performed by high performance liquid chromatography and revealed the presence of seven compounds, including caffeic acid as major constituent. The antifungal tests demonstrated that both decoction (DHm) and caffeic acid obtained from Hyptis martiusii presented MIC and MFC ≥ 4096 μg/mL against Candida albicans and Candida tropicalis strains. However, in the presence of fluconazole, DHm and caffeic acid presented IC50 of 2.60 and 2.53 μg/mL respectively, demonstrating significant synergistic effects against Candida strains. The modulator activity of DHm might be due to the presence of caffeic acid. Moreover, DHm and caffeic acid did not cause significant hemolytic effects, indicating that they present low cytotoxicity. Conclusions: These data indicate that DHm potentiates the activity of the fluconazole, without enhancement of the toxicity, encouraging further toxicological, pharmacological and phytochemical studies to provide consistent evidence of the potential of this plant to be used in drug development. |
format |
Article in Journal/Newspaper |
author |
Janaína Esmeraldo Rocha Henrique Douglas Melo Coutinho Cícero Roberto Nascimento Saraiva Antonia Thassya Lucas dos Santos Antônio Judson Targino Machado João Tavares Calixto Júnior Irwin Rose Alencar Menezes José Galberto Martins da Costa Jaime Ribeiro-Filho Aracelio Viana Colares |
author_facet |
Janaína Esmeraldo Rocha Henrique Douglas Melo Coutinho Cícero Roberto Nascimento Saraiva Antonia Thassya Lucas dos Santos Antônio Judson Targino Machado João Tavares Calixto Júnior Irwin Rose Alencar Menezes José Galberto Martins da Costa Jaime Ribeiro-Filho Aracelio Viana Colares |
author_sort |
Janaína Esmeraldo Rocha |
title |
HPLC-DAD analysis and antifungal effect of Hyptis martiusii Benth (Lamiaceae) against Candida strains |
title_short |
HPLC-DAD analysis and antifungal effect of Hyptis martiusii Benth (Lamiaceae) against Candida strains |
title_full |
HPLC-DAD analysis and antifungal effect of Hyptis martiusii Benth (Lamiaceae) against Candida strains |
title_fullStr |
HPLC-DAD analysis and antifungal effect of Hyptis martiusii Benth (Lamiaceae) against Candida strains |
title_full_unstemmed |
HPLC-DAD analysis and antifungal effect of Hyptis martiusii Benth (Lamiaceae) against Candida strains |
title_sort |
hplc-dad analysis and antifungal effect of hyptis martiusii benth (lamiaceae) against candida strains |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/2221-1691.254606 https://doaj.org/article/e6502fff0dd043bcb75358f623d89f94 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 3, Pp 123-128 (2019) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=3;spage=123;epage=128;aulast=Rocha https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.254606 https://doaj.org/article/e6502fff0dd043bcb75358f623d89f94 |
op_doi |
https://doi.org/10.4103/2221-1691.254606 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
9 |
container_issue |
3 |
container_start_page |
123 |
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1766342286463991808 |