Wolbachia induces density-dependent inhibition to dengue virus in mosquito cells.
Wolbachia is a maternal transmitted endosymbiotic bacterium that is estimated to infect up to 65% of insect species. The ability of Wolbachia to both induce viral interference and spread into mosquito vector population makes it possible to develop Wolbachia as a biological control agent for dengue c...
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ftdoajarticles:oai:doaj.org/article:e63bb656da304862b9ee3504e9c64361 2023-05-15T15:12:27+02:00 Wolbachia induces density-dependent inhibition to dengue virus in mosquito cells. Peng Lu Guowu Bian Xiaoling Pan Zhiyong Xi 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001754 https://doaj.org/article/e63bb656da304862b9ee3504e9c64361 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3404113?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001754 https://doaj.org/article/e63bb656da304862b9ee3504e9c64361 PLoS Neglected Tropical Diseases, Vol 6, Iss 7, p e1754 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001754 2022-12-31T03:38:12Z Wolbachia is a maternal transmitted endosymbiotic bacterium that is estimated to infect up to 65% of insect species. The ability of Wolbachia to both induce viral interference and spread into mosquito vector population makes it possible to develop Wolbachia as a biological control agent for dengue control. While Wolbachia induces resistance to dengue virus in the transinfected Aedes aegypti mosquitoes, a similar effect was not observed in Aedes albopictus, which naturally carries Wolbachia infection but still serves as a dengue vector. In order to understand the mechanism of this lack of Wolbachia-mediated viral interference, we used both Ae. albopictus cell line (Aa23) and mosquitoes to characterize the impact of Wolbachia on dengue infection. A serial of sub-lethal doses of antibiotic treatment was used to partially remove Wolbachia in Aa23 cells and generate cell cultures with Wolbachia at different densities. We show that there is a strong negative linear correlation between the genome copy of Wolbachia and dengue virus with a dengue infection completely removed when Wolbacha density reaches a certain level. We then compared Wolbachia density between transinfected Ae. aegypti and naturally infected Ae. albopictus. The results show that Wolbachia density in midgut, fatbody and salivary gland of Ae. albopictus is 80-, 18-, and 24-fold less than that of Ae. aegypti, respectively. We provide evidence that Wolbachia density in somatic tissues of Ae. albopictus is too low to induce resistance to dengue virus. Our results will aid in understanding the mechanism of Wolbachia-mediated pathogen interference and developing novel methods to block disease transmission by mosquitoes carrying native Wolbachia infections. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 7 e1754 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Peng Lu Guowu Bian Xiaoling Pan Zhiyong Xi Wolbachia induces density-dependent inhibition to dengue virus in mosquito cells. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Wolbachia is a maternal transmitted endosymbiotic bacterium that is estimated to infect up to 65% of insect species. The ability of Wolbachia to both induce viral interference and spread into mosquito vector population makes it possible to develop Wolbachia as a biological control agent for dengue control. While Wolbachia induces resistance to dengue virus in the transinfected Aedes aegypti mosquitoes, a similar effect was not observed in Aedes albopictus, which naturally carries Wolbachia infection but still serves as a dengue vector. In order to understand the mechanism of this lack of Wolbachia-mediated viral interference, we used both Ae. albopictus cell line (Aa23) and mosquitoes to characterize the impact of Wolbachia on dengue infection. A serial of sub-lethal doses of antibiotic treatment was used to partially remove Wolbachia in Aa23 cells and generate cell cultures with Wolbachia at different densities. We show that there is a strong negative linear correlation between the genome copy of Wolbachia and dengue virus with a dengue infection completely removed when Wolbacha density reaches a certain level. We then compared Wolbachia density between transinfected Ae. aegypti and naturally infected Ae. albopictus. The results show that Wolbachia density in midgut, fatbody and salivary gland of Ae. albopictus is 80-, 18-, and 24-fold less than that of Ae. aegypti, respectively. We provide evidence that Wolbachia density in somatic tissues of Ae. albopictus is too low to induce resistance to dengue virus. Our results will aid in understanding the mechanism of Wolbachia-mediated pathogen interference and developing novel methods to block disease transmission by mosquitoes carrying native Wolbachia infections. |
format |
Article in Journal/Newspaper |
author |
Peng Lu Guowu Bian Xiaoling Pan Zhiyong Xi |
author_facet |
Peng Lu Guowu Bian Xiaoling Pan Zhiyong Xi |
author_sort |
Peng Lu |
title |
Wolbachia induces density-dependent inhibition to dengue virus in mosquito cells. |
title_short |
Wolbachia induces density-dependent inhibition to dengue virus in mosquito cells. |
title_full |
Wolbachia induces density-dependent inhibition to dengue virus in mosquito cells. |
title_fullStr |
Wolbachia induces density-dependent inhibition to dengue virus in mosquito cells. |
title_full_unstemmed |
Wolbachia induces density-dependent inhibition to dengue virus in mosquito cells. |
title_sort |
wolbachia induces density-dependent inhibition to dengue virus in mosquito cells. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001754 https://doaj.org/article/e63bb656da304862b9ee3504e9c64361 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 7, p e1754 (2012) |
op_relation |
http://europepmc.org/articles/PMC3404113?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001754 https://doaj.org/article/e63bb656da304862b9ee3504e9c64361 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001754 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
6 |
container_issue |
7 |
container_start_page |
e1754 |
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1766343124582400000 |