Lack of Restoration in Vivo by K+-Channel Modulators of Jejunal Fluid Absorption after Heat Stable Escherichia coli Enterotoxin (STa) Challenge
Enhanced potassium ion permeability at the enterocyte basolateral membrane is assumed to facilitate sustained chloride ion and fluid secretion into the intestinal lumen during episodes of secretory diarrhoeal disease. To examine this concept in vivo, two potassium ion channel blockers and a channel...
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ftdoajarticles:oai:doaj.org/article:e627268d31a548d7b6555ea6698422e1 2023-05-15T15:09:48+02:00 Lack of Restoration in Vivo by K+-Channel Modulators of Jejunal Fluid Absorption after Heat Stable Escherichia coli Enterotoxin (STa) Challenge M. L. Lucas L. C. Gilligan C. C. Whitelaw P. J. Wynne J. D. Morrison 2011-01-01T00:00:00Z https://doi.org/10.1155/2011/853686 https://doaj.org/article/e627268d31a548d7b6555ea6698422e1 EN eng Hindawi Limited http://dx.doi.org/10.1155/2011/853686 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2011/853686 https://doaj.org/article/e627268d31a548d7b6555ea6698422e1 Journal of Tropical Medicine, Vol 2011 (2011) Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles https://doi.org/10.1155/2011/853686 2022-12-31T13:20:28Z Enhanced potassium ion permeability at the enterocyte basolateral membrane is assumed to facilitate sustained chloride ion and fluid secretion into the intestinal lumen during episodes of secretory diarrhoeal disease. To examine this concept in vivo, two potassium ion channel blockers and a channel opener were coperfused with E. coli heat stable STa enterotoxin to determine whether such compounds improved or worsened the inhibited fluid absorption. In the STa (80 ng/mL) challenged jejunal loop, the fluid absorption rate of 28.6 ± 5.8 (14) μL/cm/hr was significantly below (P<.001) the normal rate of 98.8 ± 6.2 (17) μL/cm/hr. Intraluminal (300 uM) glibenclamide added to STa perfused loops failed to improve the inhibited fluid absorption rate, which was 7.4 ± 3.2 (6) μL/cm/hr on coperfusion with STa. Similarly, on coperfusion with 30 uM clotrimazole, the fluid absorption rate with STa present remained inhibited at 11.4 ± 7.0 (4) μL/cm/hr. On coperfusion with intraluminal 1 uM cromakalim, STa reduced fluid absorption significantly (P<.02) to 24.7 ± 8.0 (10) μL/cm/hr, no different from STa challenge in the absence of cromakalim. Infusion i.v. with these agents also failed to restore fluid absorption after STa challenge. These observations do not support the proposed potassium ion permeability event as a necessary corollary of enterotoxin-mediated secretion. This makes it unlikely that modulators of such permeability prevent enterocyte secretion in diarrhoeal disease. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2011 1 7 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 M. L. Lucas L. C. Gilligan C. C. Whitelaw P. J. Wynne J. D. Morrison Lack of Restoration in Vivo by K+-Channel Modulators of Jejunal Fluid Absorption after Heat Stable Escherichia coli Enterotoxin (STa) Challenge |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
Enhanced potassium ion permeability at the enterocyte basolateral membrane is assumed to facilitate sustained chloride ion and fluid secretion into the intestinal lumen during episodes of secretory diarrhoeal disease. To examine this concept in vivo, two potassium ion channel blockers and a channel opener were coperfused with E. coli heat stable STa enterotoxin to determine whether such compounds improved or worsened the inhibited fluid absorption. In the STa (80 ng/mL) challenged jejunal loop, the fluid absorption rate of 28.6 ± 5.8 (14) μL/cm/hr was significantly below (P<.001) the normal rate of 98.8 ± 6.2 (17) μL/cm/hr. Intraluminal (300 uM) glibenclamide added to STa perfused loops failed to improve the inhibited fluid absorption rate, which was 7.4 ± 3.2 (6) μL/cm/hr on coperfusion with STa. Similarly, on coperfusion with 30 uM clotrimazole, the fluid absorption rate with STa present remained inhibited at 11.4 ± 7.0 (4) μL/cm/hr. On coperfusion with intraluminal 1 uM cromakalim, STa reduced fluid absorption significantly (P<.02) to 24.7 ± 8.0 (10) μL/cm/hr, no different from STa challenge in the absence of cromakalim. Infusion i.v. with these agents also failed to restore fluid absorption after STa challenge. These observations do not support the proposed potassium ion permeability event as a necessary corollary of enterotoxin-mediated secretion. This makes it unlikely that modulators of such permeability prevent enterocyte secretion in diarrhoeal disease. |
format |
Article in Journal/Newspaper |
author |
M. L. Lucas L. C. Gilligan C. C. Whitelaw P. J. Wynne J. D. Morrison |
author_facet |
M. L. Lucas L. C. Gilligan C. C. Whitelaw P. J. Wynne J. D. Morrison |
author_sort |
M. L. Lucas |
title |
Lack of Restoration in Vivo by K+-Channel Modulators of Jejunal Fluid Absorption after Heat Stable Escherichia coli Enterotoxin (STa) Challenge |
title_short |
Lack of Restoration in Vivo by K+-Channel Modulators of Jejunal Fluid Absorption after Heat Stable Escherichia coli Enterotoxin (STa) Challenge |
title_full |
Lack of Restoration in Vivo by K+-Channel Modulators of Jejunal Fluid Absorption after Heat Stable Escherichia coli Enterotoxin (STa) Challenge |
title_fullStr |
Lack of Restoration in Vivo by K+-Channel Modulators of Jejunal Fluid Absorption after Heat Stable Escherichia coli Enterotoxin (STa) Challenge |
title_full_unstemmed |
Lack of Restoration in Vivo by K+-Channel Modulators of Jejunal Fluid Absorption after Heat Stable Escherichia coli Enterotoxin (STa) Challenge |
title_sort |
lack of restoration in vivo by k+-channel modulators of jejunal fluid absorption after heat stable escherichia coli enterotoxin (sta) challenge |
publisher |
Hindawi Limited |
publishDate |
2011 |
url |
https://doi.org/10.1155/2011/853686 https://doaj.org/article/e627268d31a548d7b6555ea6698422e1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2011 (2011) |
op_relation |
http://dx.doi.org/10.1155/2011/853686 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2011/853686 https://doaj.org/article/e627268d31a548d7b6555ea6698422e1 |
op_doi |
https://doi.org/10.1155/2011/853686 |
container_title |
Journal of Tropical Medicine |
container_volume |
2011 |
container_start_page |
1 |
op_container_end_page |
7 |
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1766340914106597376 |