Chagas' disease: IgA, IgM and IgG antibodies to T. cruzi amastigote, trypomastigote and epimastigote antigens in acute and in different chronic forms of the disease Doença de Chagas: anticorpos IgG, IgM e IgA contra antígenos de amastigota, tripomastigota e epimastigota de T. cruzi em formas agudas e em diferentes formas crônicas da doença
In an attempt to find a better T. cruzi antigen and possible immunological markers for the diagnosis of different clinical forms of Chagas' disease, amastigote and trypomastigote antigens obtained from immunosuppressed mice infected with T. cruzi (Y strain) were assessed in comparison with conv...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
1990
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ftdoajarticles:oai:doaj.org/article:e61a84401cb846be8a58cd00c569b7bf 2024-09-09T19:28:38+00:00 Chagas' disease: IgA, IgM and IgG antibodies to T. cruzi amastigote, trypomastigote and epimastigote antigens in acute and in different chronic forms of the disease Doença de Chagas: anticorpos IgG, IgM e IgA contra antígenos de amastigota, tripomastigota e epimastigota de T. cruzi em formas agudas e em diferentes formas crônicas da doença Kátia S. C. Primavera Eufrosina S. Umezawa Benedito Anselmo Peres Mário E. Camargo Sumie Hoshino-Shimuzu 1990-06-01T00:00:00Z https://doi.org/10.1590/S0036-46651990000300005 https://doaj.org/article/e61a84401cb846be8a58cd00c569b7bf EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651990000300005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651990000300005 0036-4665 1678-9946 https://doaj.org/article/e61a84401cb846be8a58cd00c569b7bf Revista do Instituto de Medicina Tropical de São Paulo, Vol 32, Iss 3, Pp 172-180 (1990) T. cruzi infections IgG IgM and IgA antibodies Amastigote Trypomastigote and Epimastigote Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1990 ftdoajarticles https://doi.org/10.1590/S0036-46651990000300005 2024-08-05T17:49:30Z In an attempt to find a better T. cruzi antigen and possible immunological markers for the diagnosis of different clinical forms of Chagas' disease, amastigote and trypomastigote antigens obtained from immunosuppressed mice infected with T. cruzi (Y strain) were assessed in comparison with conventional epimastigote antigens. A total of 506 serum samples from patients with acute and with chronic (indeterminate, cardiac and digestive) forms, from nonchagasic infections, and from healthy individuals were assayed in immunofluorescence (IF) tests, to search for IgG, IgM and IgA antibodies. Amastigote proved to be the most convenient antigen for our purposes, providing higher relative efficiency indexes of 0.946, 0.871 and 0.914 for IgG, IgM and IgA IF tests, respectively. Anti-amastigote antibodies presented higher geometric mean titers (GMT) than anti-trypomastigote and anti-epimastigote. Anti-amastigote IgG antibodies were found in all forms of Chagas' disease, and predominantly IgA antibodies, in chronic digestive and in acute forms, as well as IgM antibodies, in latter forms. Thus, tests with amastigote antigen could be helpful for screening chagasic infections in blood banks. Practical and economical aspects in obtaining amastigotes as here described speak in favour of its use in developing countries, since those from other sources require more complex system of substruction, specialized personnel or equipment. Com o intúito de se aperfeiçoar o diagnóstico sorológico das diferentes formas clínicas da doença de Chagas, foram estudados antígenos de formas amastigota e tripomastigota, obtidas de camundongos imunossuprimidos infectados com cepa Y de T. cruzi, em comparação com o de epimastigota convencionalmente utilizado. Um total de 506 amostras de soro de pacientes chagásicos com formas aguda e crônicas (indeterminada, cardíaca e digestiva), de indivíduos com infecções não relacionadas e de indivíduos sadios foi analisado por reação de imunofluorescência, para detecção de anticorpos IgG, IgM e IgA. O antígeno de ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Aguda ENVELOPE(-26.750,-26.750,-76.150,-76.150) Arctic Revista do Instituto de Medicina Tropical de São Paulo 32 3 172 180 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
T. cruzi infections IgG IgM and IgA antibodies Amastigote Trypomastigote and Epimastigote Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
T. cruzi infections IgG IgM and IgA antibodies Amastigote Trypomastigote and Epimastigote Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Kátia S. C. Primavera Eufrosina S. Umezawa Benedito Anselmo Peres Mário E. Camargo Sumie Hoshino-Shimuzu Chagas' disease: IgA, IgM and IgG antibodies to T. cruzi amastigote, trypomastigote and epimastigote antigens in acute and in different chronic forms of the disease Doença de Chagas: anticorpos IgG, IgM e IgA contra antígenos de amastigota, tripomastigota e epimastigota de T. cruzi em formas agudas e em diferentes formas crônicas da doença |
topic_facet |
T. cruzi infections IgG IgM and IgA antibodies Amastigote Trypomastigote and Epimastigote Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
In an attempt to find a better T. cruzi antigen and possible immunological markers for the diagnosis of different clinical forms of Chagas' disease, amastigote and trypomastigote antigens obtained from immunosuppressed mice infected with T. cruzi (Y strain) were assessed in comparison with conventional epimastigote antigens. A total of 506 serum samples from patients with acute and with chronic (indeterminate, cardiac and digestive) forms, from nonchagasic infections, and from healthy individuals were assayed in immunofluorescence (IF) tests, to search for IgG, IgM and IgA antibodies. Amastigote proved to be the most convenient antigen for our purposes, providing higher relative efficiency indexes of 0.946, 0.871 and 0.914 for IgG, IgM and IgA IF tests, respectively. Anti-amastigote antibodies presented higher geometric mean titers (GMT) than anti-trypomastigote and anti-epimastigote. Anti-amastigote IgG antibodies were found in all forms of Chagas' disease, and predominantly IgA antibodies, in chronic digestive and in acute forms, as well as IgM antibodies, in latter forms. Thus, tests with amastigote antigen could be helpful for screening chagasic infections in blood banks. Practical and economical aspects in obtaining amastigotes as here described speak in favour of its use in developing countries, since those from other sources require more complex system of substruction, specialized personnel or equipment. Com o intúito de se aperfeiçoar o diagnóstico sorológico das diferentes formas clínicas da doença de Chagas, foram estudados antígenos de formas amastigota e tripomastigota, obtidas de camundongos imunossuprimidos infectados com cepa Y de T. cruzi, em comparação com o de epimastigota convencionalmente utilizado. Um total de 506 amostras de soro de pacientes chagásicos com formas aguda e crônicas (indeterminada, cardíaca e digestiva), de indivíduos com infecções não relacionadas e de indivíduos sadios foi analisado por reação de imunofluorescência, para detecção de anticorpos IgG, IgM e IgA. O antígeno de ... |
format |
Article in Journal/Newspaper |
author |
Kátia S. C. Primavera Eufrosina S. Umezawa Benedito Anselmo Peres Mário E. Camargo Sumie Hoshino-Shimuzu |
author_facet |
Kátia S. C. Primavera Eufrosina S. Umezawa Benedito Anselmo Peres Mário E. Camargo Sumie Hoshino-Shimuzu |
author_sort |
Kátia S. C. Primavera |
title |
Chagas' disease: IgA, IgM and IgG antibodies to T. cruzi amastigote, trypomastigote and epimastigote antigens in acute and in different chronic forms of the disease Doença de Chagas: anticorpos IgG, IgM e IgA contra antígenos de amastigota, tripomastigota e epimastigota de T. cruzi em formas agudas e em diferentes formas crônicas da doença |
title_short |
Chagas' disease: IgA, IgM and IgG antibodies to T. cruzi amastigote, trypomastigote and epimastigote antigens in acute and in different chronic forms of the disease Doença de Chagas: anticorpos IgG, IgM e IgA contra antígenos de amastigota, tripomastigota e epimastigota de T. cruzi em formas agudas e em diferentes formas crônicas da doença |
title_full |
Chagas' disease: IgA, IgM and IgG antibodies to T. cruzi amastigote, trypomastigote and epimastigote antigens in acute and in different chronic forms of the disease Doença de Chagas: anticorpos IgG, IgM e IgA contra antígenos de amastigota, tripomastigota e epimastigota de T. cruzi em formas agudas e em diferentes formas crônicas da doença |
title_fullStr |
Chagas' disease: IgA, IgM and IgG antibodies to T. cruzi amastigote, trypomastigote and epimastigote antigens in acute and in different chronic forms of the disease Doença de Chagas: anticorpos IgG, IgM e IgA contra antígenos de amastigota, tripomastigota e epimastigota de T. cruzi em formas agudas e em diferentes formas crônicas da doença |
title_full_unstemmed |
Chagas' disease: IgA, IgM and IgG antibodies to T. cruzi amastigote, trypomastigote and epimastigote antigens in acute and in different chronic forms of the disease Doença de Chagas: anticorpos IgG, IgM e IgA contra antígenos de amastigota, tripomastigota e epimastigota de T. cruzi em formas agudas e em diferentes formas crônicas da doença |
title_sort |
chagas' disease: iga, igm and igg antibodies to t. cruzi amastigote, trypomastigote and epimastigote antigens in acute and in different chronic forms of the disease doença de chagas: anticorpos igg, igm e iga contra antígenos de amastigota, tripomastigota e epimastigota de t. cruzi em formas agudas e em diferentes formas crônicas da doença |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1990 |
url |
https://doi.org/10.1590/S0036-46651990000300005 https://doaj.org/article/e61a84401cb846be8a58cd00c569b7bf |
long_lat |
ENVELOPE(-26.750,-26.750,-76.150,-76.150) |
geographic |
Aguda Arctic |
geographic_facet |
Aguda Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 32, Iss 3, Pp 172-180 (1990) |
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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651990000300005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651990000300005 0036-4665 1678-9946 https://doaj.org/article/e61a84401cb846be8a58cd00c569b7bf |
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https://doi.org/10.1590/S0036-46651990000300005 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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32 |
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