UPLC/ESI/MS profiling of red algae Galaxaura rugosa extracts and its activity against malaria mosquito vector, Anopheles pharoensis, with reference to Danio rerio and Daphnia magna as bioindicators
Abstract Background Anopheles pharoensis has a major role in transmitting several human diseases, especially malaria, in Egypt?. Controlling Anopheles is considered as an effective strategy to eliminate the spread of malaria worldwide. Galaxaura rugosa is a species of red algae found in tropical to...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2023
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12936-023-04795-w https://doaj.org/article/e616faa256614f688c5a2f07e804ffb8 |
id |
ftdoajarticles:oai:doaj.org/article:e616faa256614f688c5a2f07e804ffb8 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:e616faa256614f688c5a2f07e804ffb8 2024-01-07T09:42:01+01:00 UPLC/ESI/MS profiling of red algae Galaxaura rugosa extracts and its activity against malaria mosquito vector, Anopheles pharoensis, with reference to Danio rerio and Daphnia magna as bioindicators Mohamed A. M. El-Tabakh Esraa A. Elhawary Hossam M. Hwihy Kareem F. Darweesh Raafat M. Shaapan Emad A. Ghazala Mostafa M. Mokhtar Hassan O. Waheeb Deyaa E. M. Emam Nader A. Bakr Ahmed Z. I. Shehata 2023-12-01T00:00:00Z https://doi.org/10.1186/s12936-023-04795-w https://doaj.org/article/e616faa256614f688c5a2f07e804ffb8 EN eng BMC https://doi.org/10.1186/s12936-023-04795-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-023-04795-w 1475-2875 https://doaj.org/article/e616faa256614f688c5a2f07e804ffb8 Malaria Journal, Vol 22, Iss 1, Pp 1-16 (2023) Galaxaura rugosa Anopheles pharoensis Extract Larvicidal LCMS Metabolomics Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2023 ftdoajarticles https://doi.org/10.1186/s12936-023-04795-w 2023-12-10T01:48:34Z Abstract Background Anopheles pharoensis has a major role in transmitting several human diseases, especially malaria, in Egypt?. Controlling Anopheles is considered as an effective strategy to eliminate the spread of malaria worldwide. Galaxaura rugosa is a species of red algae found in tropical to subtropical marine environments. The presence of G. rugosa is indicative of the ecosystem's overall health. The current work aims to investigate UPLC/ESI/MS profile of G. rugosa methanol and petroleum ether extracts and its activity against An. pharoensis and non-target organisms, Danio rerio and Daphnia magna. Methods Galaxaura rugosa specimens have been identified using DNA barcoding for the COI gene and verified as G. rugosa. The UPLC/ESI/MS profiling of G. rugosa collected from Egypt was described. The larvicidal and repellent activities of G. rugosa methanol and petroleum ether extracts against An. pharoensis were evaluated, as well as the toxicity of tested extracts on non-target organisms, Dan. rerio and Dap. magna. Results The UPLC/ESI/MS analysis of methanol and petroleum ether extracts led to the tentative identification of 57 compounds belonging to different phytochemical classes, including flavonoids, tannins, phenolic acids, phenyl propanoids. Larval mortality was recorded at 93.33% and 90.67% at 80 and 35 ppm of methanol and petroleum ether extracts, respectively, while pupal mortality recorded 44.44 and 22.48% at 35 and 30 ppm, respectively. Larval duration was recorded at 5.31 and 5.64 days by methanol and petroleum ether extracts at 80 and 35 ppm, respectively. A decrease in acetylcholinesterase (AChE) level and a promotion in Glutathione-S-transferase (GST) level of An. pharoensis 3rd instar larvae were recorded by tested extracts. The petroleum ether extract was more effective against An. pharoensis starved females than methanol extract. Also, tested extracts recorded LC50 of 1988.8, 1365.1, and 11.65, 14.36 µg/mL against Dan. rerio, and Dap. magna, respectively. Conclusions Using red algae ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Rugosa ENVELOPE(-61.250,-61.250,-62.633,-62.633) Malaria Journal 22 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Galaxaura rugosa Anopheles pharoensis Extract Larvicidal LCMS Metabolomics Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Galaxaura rugosa Anopheles pharoensis Extract Larvicidal LCMS Metabolomics Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mohamed A. M. El-Tabakh Esraa A. Elhawary Hossam M. Hwihy Kareem F. Darweesh Raafat M. Shaapan Emad A. Ghazala Mostafa M. Mokhtar Hassan O. Waheeb Deyaa E. M. Emam Nader A. Bakr Ahmed Z. I. Shehata UPLC/ESI/MS profiling of red algae Galaxaura rugosa extracts and its activity against malaria mosquito vector, Anopheles pharoensis, with reference to Danio rerio and Daphnia magna as bioindicators |
topic_facet |
Galaxaura rugosa Anopheles pharoensis Extract Larvicidal LCMS Metabolomics Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Anopheles pharoensis has a major role in transmitting several human diseases, especially malaria, in Egypt?. Controlling Anopheles is considered as an effective strategy to eliminate the spread of malaria worldwide. Galaxaura rugosa is a species of red algae found in tropical to subtropical marine environments. The presence of G. rugosa is indicative of the ecosystem's overall health. The current work aims to investigate UPLC/ESI/MS profile of G. rugosa methanol and petroleum ether extracts and its activity against An. pharoensis and non-target organisms, Danio rerio and Daphnia magna. Methods Galaxaura rugosa specimens have been identified using DNA barcoding for the COI gene and verified as G. rugosa. The UPLC/ESI/MS profiling of G. rugosa collected from Egypt was described. The larvicidal and repellent activities of G. rugosa methanol and petroleum ether extracts against An. pharoensis were evaluated, as well as the toxicity of tested extracts on non-target organisms, Dan. rerio and Dap. magna. Results The UPLC/ESI/MS analysis of methanol and petroleum ether extracts led to the tentative identification of 57 compounds belonging to different phytochemical classes, including flavonoids, tannins, phenolic acids, phenyl propanoids. Larval mortality was recorded at 93.33% and 90.67% at 80 and 35 ppm of methanol and petroleum ether extracts, respectively, while pupal mortality recorded 44.44 and 22.48% at 35 and 30 ppm, respectively. Larval duration was recorded at 5.31 and 5.64 days by methanol and petroleum ether extracts at 80 and 35 ppm, respectively. A decrease in acetylcholinesterase (AChE) level and a promotion in Glutathione-S-transferase (GST) level of An. pharoensis 3rd instar larvae were recorded by tested extracts. The petroleum ether extract was more effective against An. pharoensis starved females than methanol extract. Also, tested extracts recorded LC50 of 1988.8, 1365.1, and 11.65, 14.36 µg/mL against Dan. rerio, and Dap. magna, respectively. Conclusions Using red algae ... |
format |
Article in Journal/Newspaper |
author |
Mohamed A. M. El-Tabakh Esraa A. Elhawary Hossam M. Hwihy Kareem F. Darweesh Raafat M. Shaapan Emad A. Ghazala Mostafa M. Mokhtar Hassan O. Waheeb Deyaa E. M. Emam Nader A. Bakr Ahmed Z. I. Shehata |
author_facet |
Mohamed A. M. El-Tabakh Esraa A. Elhawary Hossam M. Hwihy Kareem F. Darweesh Raafat M. Shaapan Emad A. Ghazala Mostafa M. Mokhtar Hassan O. Waheeb Deyaa E. M. Emam Nader A. Bakr Ahmed Z. I. Shehata |
author_sort |
Mohamed A. M. El-Tabakh |
title |
UPLC/ESI/MS profiling of red algae Galaxaura rugosa extracts and its activity against malaria mosquito vector, Anopheles pharoensis, with reference to Danio rerio and Daphnia magna as bioindicators |
title_short |
UPLC/ESI/MS profiling of red algae Galaxaura rugosa extracts and its activity against malaria mosquito vector, Anopheles pharoensis, with reference to Danio rerio and Daphnia magna as bioindicators |
title_full |
UPLC/ESI/MS profiling of red algae Galaxaura rugosa extracts and its activity against malaria mosquito vector, Anopheles pharoensis, with reference to Danio rerio and Daphnia magna as bioindicators |
title_fullStr |
UPLC/ESI/MS profiling of red algae Galaxaura rugosa extracts and its activity against malaria mosquito vector, Anopheles pharoensis, with reference to Danio rerio and Daphnia magna as bioindicators |
title_full_unstemmed |
UPLC/ESI/MS profiling of red algae Galaxaura rugosa extracts and its activity against malaria mosquito vector, Anopheles pharoensis, with reference to Danio rerio and Daphnia magna as bioindicators |
title_sort |
uplc/esi/ms profiling of red algae galaxaura rugosa extracts and its activity against malaria mosquito vector, anopheles pharoensis, with reference to danio rerio and daphnia magna as bioindicators |
publisher |
BMC |
publishDate |
2023 |
url |
https://doi.org/10.1186/s12936-023-04795-w https://doaj.org/article/e616faa256614f688c5a2f07e804ffb8 |
long_lat |
ENVELOPE(-61.250,-61.250,-62.633,-62.633) |
geographic |
Arctic Rugosa |
geographic_facet |
Arctic Rugosa |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 22, Iss 1, Pp 1-16 (2023) |
op_relation |
https://doi.org/10.1186/s12936-023-04795-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-023-04795-w 1475-2875 https://doaj.org/article/e616faa256614f688c5a2f07e804ffb8 |
op_doi |
https://doi.org/10.1186/s12936-023-04795-w |
container_title |
Malaria Journal |
container_volume |
22 |
container_issue |
1 |
_version_ |
1787422856951365632 |