The Biodiversity of Calcaxonian Octocorals from the Irish Continental Slope Inferred from Multilocus Mitochondrial Barcoding
Deep-sea corals are important benthic inhabitants that support the biodiversity and function of the wider faunal community; however, their taxonomy is underdeveloped and their accurate identification is often difficult. In our study, we investigated the utility of a superextended (>3000 bp) barco...
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ftdoajarticles:oai:doaj.org/article:e607da604660470e8ad6ea5add2897c0 2023-05-15T17:41:36+02:00 The Biodiversity of Calcaxonian Octocorals from the Irish Continental Slope Inferred from Multilocus Mitochondrial Barcoding Declan Morrissey Candice B. Untiedt Karen Croke Aisling Robinson Eva Turley A. Louise Allcock 2022-07-01T00:00:00Z https://doi.org/10.3390/d14070576 https://doaj.org/article/e607da604660470e8ad6ea5add2897c0 EN eng MDPI AG https://www.mdpi.com/1424-2818/14/7/576 https://doaj.org/toc/1424-2818 doi:10.3390/d14070576 1424-2818 https://doaj.org/article/e607da604660470e8ad6ea5add2897c0 Diversity, Vol 14, Iss 576, p 576 (2022) Keratoisididae Primnoidae Chrysogorgiidae Chelidonisididae deep sea species delimitation Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/d14070576 2022-12-30T20:00:22Z Deep-sea corals are important benthic inhabitants that support the biodiversity and function of the wider faunal community; however, their taxonomy is underdeveloped and their accurate identification is often difficult. In our study, we investigated the utility of a superextended (>3000 bp) barcode and explored the effectiveness of various molecular species delimitation techniques with an aim to put upper and lower bounds on the estimated number of calcaxonian species in Irish waters. We collected 112 calcaxonians (70 Keratoisididae, 22 Primnoidae, 20 Chrysogorgiidae) and one chelidonisid from the Irish continental slope and sequenced a 3390 bp DNA barcode comprising four mitochondrial regions ( mtMutS , COI + igr1, 16S rRNA- ND2 , and igr4), recovering 38 haplotypes. Individuals that shared a haplotype were often morphologically distinct, and we thus undertook detailed morphological work, including SEM of sclerites, on one representative of each morphotype within each haplotype. GMYC, bGMYC, and mPTP returned incongruent estimates of species numbers. In total, there are between 25 and 40 species, although no definitive number could be assigned, primarily due to poorly defined keratoisidid species boundaries. As expected, the superextended barcode provided greater discrimination power than single markers; bGMYC appeared to be the most effective delimiter. Among the identified species were Chelidonisis aurantiaca , collected deeper than previously known at 1507 m, and Calyptrophora clinata, recorded for the second time from the Northeast Atlantic. A full understanding of the diversity and distribution of calcaxonians requires substantial taxonomic work, but we highlight the Irish continental slope as harbouring significant diversity. Article in Journal/Newspaper Northeast Atlantic Directory of Open Access Journals: DOAJ Articles Diversity 14 7 576 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Keratoisididae Primnoidae Chrysogorgiidae Chelidonisididae deep sea species delimitation Biology (General) QH301-705.5 |
spellingShingle |
Keratoisididae Primnoidae Chrysogorgiidae Chelidonisididae deep sea species delimitation Biology (General) QH301-705.5 Declan Morrissey Candice B. Untiedt Karen Croke Aisling Robinson Eva Turley A. Louise Allcock The Biodiversity of Calcaxonian Octocorals from the Irish Continental Slope Inferred from Multilocus Mitochondrial Barcoding |
topic_facet |
Keratoisididae Primnoidae Chrysogorgiidae Chelidonisididae deep sea species delimitation Biology (General) QH301-705.5 |
description |
Deep-sea corals are important benthic inhabitants that support the biodiversity and function of the wider faunal community; however, their taxonomy is underdeveloped and their accurate identification is often difficult. In our study, we investigated the utility of a superextended (>3000 bp) barcode and explored the effectiveness of various molecular species delimitation techniques with an aim to put upper and lower bounds on the estimated number of calcaxonian species in Irish waters. We collected 112 calcaxonians (70 Keratoisididae, 22 Primnoidae, 20 Chrysogorgiidae) and one chelidonisid from the Irish continental slope and sequenced a 3390 bp DNA barcode comprising four mitochondrial regions ( mtMutS , COI + igr1, 16S rRNA- ND2 , and igr4), recovering 38 haplotypes. Individuals that shared a haplotype were often morphologically distinct, and we thus undertook detailed morphological work, including SEM of sclerites, on one representative of each morphotype within each haplotype. GMYC, bGMYC, and mPTP returned incongruent estimates of species numbers. In total, there are between 25 and 40 species, although no definitive number could be assigned, primarily due to poorly defined keratoisidid species boundaries. As expected, the superextended barcode provided greater discrimination power than single markers; bGMYC appeared to be the most effective delimiter. Among the identified species were Chelidonisis aurantiaca , collected deeper than previously known at 1507 m, and Calyptrophora clinata, recorded for the second time from the Northeast Atlantic. A full understanding of the diversity and distribution of calcaxonians requires substantial taxonomic work, but we highlight the Irish continental slope as harbouring significant diversity. |
format |
Article in Journal/Newspaper |
author |
Declan Morrissey Candice B. Untiedt Karen Croke Aisling Robinson Eva Turley A. Louise Allcock |
author_facet |
Declan Morrissey Candice B. Untiedt Karen Croke Aisling Robinson Eva Turley A. Louise Allcock |
author_sort |
Declan Morrissey |
title |
The Biodiversity of Calcaxonian Octocorals from the Irish Continental Slope Inferred from Multilocus Mitochondrial Barcoding |
title_short |
The Biodiversity of Calcaxonian Octocorals from the Irish Continental Slope Inferred from Multilocus Mitochondrial Barcoding |
title_full |
The Biodiversity of Calcaxonian Octocorals from the Irish Continental Slope Inferred from Multilocus Mitochondrial Barcoding |
title_fullStr |
The Biodiversity of Calcaxonian Octocorals from the Irish Continental Slope Inferred from Multilocus Mitochondrial Barcoding |
title_full_unstemmed |
The Biodiversity of Calcaxonian Octocorals from the Irish Continental Slope Inferred from Multilocus Mitochondrial Barcoding |
title_sort |
biodiversity of calcaxonian octocorals from the irish continental slope inferred from multilocus mitochondrial barcoding |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/d14070576 https://doaj.org/article/e607da604660470e8ad6ea5add2897c0 |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_source |
Diversity, Vol 14, Iss 576, p 576 (2022) |
op_relation |
https://www.mdpi.com/1424-2818/14/7/576 https://doaj.org/toc/1424-2818 doi:10.3390/d14070576 1424-2818 https://doaj.org/article/e607da604660470e8ad6ea5add2897c0 |
op_doi |
https://doi.org/10.3390/d14070576 |
container_title |
Diversity |
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14 |
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7 |
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576 |
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1766143249128357888 |