EVALUATION OF THE ANTIFUNGAL SUSCEPTIBILITY AND KARYOTYPE OF Cryptococcus neoformans ISOLATED FROM CLINICAL SAMPLES IN VENEZUELA
Thirty (30) samples coming from 27 HIV infected and non-infected patients were studied to assess antimicotic susceptibility and karyotype of Cryptococcus neoformans clinical isolates obtained in Venezuela. Identification of each sample was performed by standard methods, variety classification was ba...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2000
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ftdoajarticles:oai:doaj.org/article:e605521b3ae540ea868cb15c549c9058 2023-10-01T03:54:10+02:00 EVALUATION OF THE ANTIFUNGAL SUSCEPTIBILITY AND KARYOTYPE OF Cryptococcus neoformans ISOLATED FROM CLINICAL SAMPLES IN VENEZUELA B Calvo A Colombo O Fischman A Santiago F Nunes Branchini, M.L Branchini 2000-03-01T00:00:00Z https://doaj.org/article/e605521b3ae540ea868cb15c549c9058 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://produccioncientificaluz.org/index.php/kasmera/article/view/4671 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/e605521b3ae540ea868cb15c549c9058 Kasmera, Vol 28, Iss 3 (2000) Susceptibilidad cariotipo Cryptococcus neoformans Venezuela Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2000 ftdoajarticles 2023-09-03T00:47:38Z Thirty (30) samples coming from 27 HIV infected and non-infected patients were studied to assess antimicotic susceptibility and karyotype of Cryptococcus neoformans clinical isolates obtained in Venezuela. Identification of each sample was performed by standard methods, variety classification was based on growth in CGB agar medium, serotyping was performed by slide agglutination with five specific sera. Antifungal susceptibility was performed for amphothericin B, fluconazole, itroconazole and 5-flucytosine, according to NCCLS recommendations and karyotype study was done through contour clamped homogenous field gel electrophoresis (CHEF). Twenty seven (27) isolates of C. neoformans var. neoformans were obtained,all being of serotype A, and three (3) isolates of C. neoformans var. gattii, two (2) belonging to serotype B and one (1) to serotype C. Strains of this series were highly susceptible to the antifungal agents tested. Electrophoretic Karyotype (EK) analysis showed 13 profiles, presenting between 7 and 9 chromosomes and the molecular weight variation was 450/565 and 2200 kb. No significant differences were observed between the minimum inhibitory concentrations (MIC), nor was it possible to associate EK profiles among the isolates of HIV and non HIV patients. Further clinical and laboralorial investigations are mandatory to improve the understanding of C. neoformans in Venezuela. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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op_collection_id |
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language |
English Spanish |
topic |
Susceptibilidad cariotipo Cryptococcus neoformans Venezuela Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Susceptibilidad cariotipo Cryptococcus neoformans Venezuela Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 B Calvo A Colombo O Fischman A Santiago F Nunes Branchini, M.L Branchini EVALUATION OF THE ANTIFUNGAL SUSCEPTIBILITY AND KARYOTYPE OF Cryptococcus neoformans ISOLATED FROM CLINICAL SAMPLES IN VENEZUELA |
topic_facet |
Susceptibilidad cariotipo Cryptococcus neoformans Venezuela Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Thirty (30) samples coming from 27 HIV infected and non-infected patients were studied to assess antimicotic susceptibility and karyotype of Cryptococcus neoformans clinical isolates obtained in Venezuela. Identification of each sample was performed by standard methods, variety classification was based on growth in CGB agar medium, serotyping was performed by slide agglutination with five specific sera. Antifungal susceptibility was performed for amphothericin B, fluconazole, itroconazole and 5-flucytosine, according to NCCLS recommendations and karyotype study was done through contour clamped homogenous field gel electrophoresis (CHEF). Twenty seven (27) isolates of C. neoformans var. neoformans were obtained,all being of serotype A, and three (3) isolates of C. neoformans var. gattii, two (2) belonging to serotype B and one (1) to serotype C. Strains of this series were highly susceptible to the antifungal agents tested. Electrophoretic Karyotype (EK) analysis showed 13 profiles, presenting between 7 and 9 chromosomes and the molecular weight variation was 450/565 and 2200 kb. No significant differences were observed between the minimum inhibitory concentrations (MIC), nor was it possible to associate EK profiles among the isolates of HIV and non HIV patients. Further clinical and laboralorial investigations are mandatory to improve the understanding of C. neoformans in Venezuela. |
format |
Article in Journal/Newspaper |
author |
B Calvo A Colombo O Fischman A Santiago F Nunes Branchini, M.L Branchini |
author_facet |
B Calvo A Colombo O Fischman A Santiago F Nunes Branchini, M.L Branchini |
author_sort |
B Calvo |
title |
EVALUATION OF THE ANTIFUNGAL SUSCEPTIBILITY AND KARYOTYPE OF Cryptococcus neoformans ISOLATED FROM CLINICAL SAMPLES IN VENEZUELA |
title_short |
EVALUATION OF THE ANTIFUNGAL SUSCEPTIBILITY AND KARYOTYPE OF Cryptococcus neoformans ISOLATED FROM CLINICAL SAMPLES IN VENEZUELA |
title_full |
EVALUATION OF THE ANTIFUNGAL SUSCEPTIBILITY AND KARYOTYPE OF Cryptococcus neoformans ISOLATED FROM CLINICAL SAMPLES IN VENEZUELA |
title_fullStr |
EVALUATION OF THE ANTIFUNGAL SUSCEPTIBILITY AND KARYOTYPE OF Cryptococcus neoformans ISOLATED FROM CLINICAL SAMPLES IN VENEZUELA |
title_full_unstemmed |
EVALUATION OF THE ANTIFUNGAL SUSCEPTIBILITY AND KARYOTYPE OF Cryptococcus neoformans ISOLATED FROM CLINICAL SAMPLES IN VENEZUELA |
title_sort |
evaluation of the antifungal susceptibility and karyotype of cryptococcus neoformans isolated from clinical samples in venezuela |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2000 |
url |
https://doaj.org/article/e605521b3ae540ea868cb15c549c9058 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 28, Iss 3 (2000) |
op_relation |
https://produccioncientificaluz.org/index.php/kasmera/article/view/4671 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/e605521b3ae540ea868cb15c549c9058 |
_version_ |
1778521556777959424 |