Remarkable stability in patterns of blood-stage gene expression during episodes of non-lethal Plasmodium yoelii malaria
Abstract Background Microarray studies using in vitro cultures of synchronized, blood-stage Plasmodium falciparum malaria parasites have revealed a ‘just-in-time’ cascade of gene expression with some indication that these transcriptional patterns remain stable even in the presence of external stress...
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ftdoajarticles:oai:doaj.org/article:e5f04cf03aaf4e58ad763101612d0b60 2023-05-15T15:14:37+02:00 Remarkable stability in patterns of blood-stage gene expression during episodes of non-lethal Plasmodium yoelii malaria Cernetich-Ott Amy Daly Thomas M Vaidya Akhil B Bergman Lawrence W Burns James M 2012-08-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-265 https://doaj.org/article/e5f04cf03aaf4e58ad763101612d0b60 EN eng BMC http://www.malariajournal.com/content/11/1/265 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-265 1475-2875 https://doaj.org/article/e5f04cf03aaf4e58ad763101612d0b60 Malaria Journal, Vol 11, Iss 1, p 265 (2012) Malaria Plasmodium yoelii Blood-stage parasites DNA microarrays In vivo gene expression profiles Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-265 2022-12-30T22:19:42Z Abstract Background Microarray studies using in vitro cultures of synchronized, blood-stage Plasmodium falciparum malaria parasites have revealed a ‘just-in-time’ cascade of gene expression with some indication that these transcriptional patterns remain stable even in the presence of external stressors. However, direct analysis of transcription in P. falciparum blood-stage parasites obtained from the blood of infected patients suggests that parasite gene expression may be modulated by factors present in the in vivo environment of the host. The aim of this study was to examine changes in gene expression of the rodent malaria parasite, Plasmodium yoelii 17X, while varying the in vivo setting of replication. Methods Using P. yoelii 17X parasites replicating in vivo, differential gene expression in parasites isolated from individual mice, from independent infections, during ascending, peak and descending parasitaemia and in the presence and absence of host antibody responses was examined using P. yoelii DNA microarrays. A genome-wide analysis to identify coordinated changes in groups of genes associated with specific biological pathways was a primary focus, although an analysis of the expression patterns of two multi-gene families in P. yoelii , the yir and pyst-a families, was also completed. Results Across experimental conditions, transcription was surprisingly stable with little evidence for distinct transcriptional states or for consistent changes in specific pathways. Differential gene expression was greatest when comparing differences due to parasite load and/or host cell availability. However, the number of differentially expressed genes was generally low. Of genes that were differentially expressed, many involved biologically diverse pathways. There was little to no differential expression of members of the yir and pyst-a multigene families that encode polymorphic proteins associated with the membrane of infected erythrocytes. However, a relatively large number of these genes were expressed during ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 |
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Malaria Plasmodium yoelii Blood-stage parasites DNA microarrays In vivo gene expression profiles Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria Plasmodium yoelii Blood-stage parasites DNA microarrays In vivo gene expression profiles Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Cernetich-Ott Amy Daly Thomas M Vaidya Akhil B Bergman Lawrence W Burns James M Remarkable stability in patterns of blood-stage gene expression during episodes of non-lethal Plasmodium yoelii malaria |
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Malaria Plasmodium yoelii Blood-stage parasites DNA microarrays In vivo gene expression profiles Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Microarray studies using in vitro cultures of synchronized, blood-stage Plasmodium falciparum malaria parasites have revealed a ‘just-in-time’ cascade of gene expression with some indication that these transcriptional patterns remain stable even in the presence of external stressors. However, direct analysis of transcription in P. falciparum blood-stage parasites obtained from the blood of infected patients suggests that parasite gene expression may be modulated by factors present in the in vivo environment of the host. The aim of this study was to examine changes in gene expression of the rodent malaria parasite, Plasmodium yoelii 17X, while varying the in vivo setting of replication. Methods Using P. yoelii 17X parasites replicating in vivo, differential gene expression in parasites isolated from individual mice, from independent infections, during ascending, peak and descending parasitaemia and in the presence and absence of host antibody responses was examined using P. yoelii DNA microarrays. A genome-wide analysis to identify coordinated changes in groups of genes associated with specific biological pathways was a primary focus, although an analysis of the expression patterns of two multi-gene families in P. yoelii , the yir and pyst-a families, was also completed. Results Across experimental conditions, transcription was surprisingly stable with little evidence for distinct transcriptional states or for consistent changes in specific pathways. Differential gene expression was greatest when comparing differences due to parasite load and/or host cell availability. However, the number of differentially expressed genes was generally low. Of genes that were differentially expressed, many involved biologically diverse pathways. There was little to no differential expression of members of the yir and pyst-a multigene families that encode polymorphic proteins associated with the membrane of infected erythrocytes. However, a relatively large number of these genes were expressed during ... |
format |
Article in Journal/Newspaper |
author |
Cernetich-Ott Amy Daly Thomas M Vaidya Akhil B Bergman Lawrence W Burns James M |
author_facet |
Cernetich-Ott Amy Daly Thomas M Vaidya Akhil B Bergman Lawrence W Burns James M |
author_sort |
Cernetich-Ott Amy |
title |
Remarkable stability in patterns of blood-stage gene expression during episodes of non-lethal Plasmodium yoelii malaria |
title_short |
Remarkable stability in patterns of blood-stage gene expression during episodes of non-lethal Plasmodium yoelii malaria |
title_full |
Remarkable stability in patterns of blood-stage gene expression during episodes of non-lethal Plasmodium yoelii malaria |
title_fullStr |
Remarkable stability in patterns of blood-stage gene expression during episodes of non-lethal Plasmodium yoelii malaria |
title_full_unstemmed |
Remarkable stability in patterns of blood-stage gene expression during episodes of non-lethal Plasmodium yoelii malaria |
title_sort |
remarkable stability in patterns of blood-stage gene expression during episodes of non-lethal plasmodium yoelii malaria |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-265 https://doaj.org/article/e5f04cf03aaf4e58ad763101612d0b60 |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 265 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/265 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-265 1475-2875 https://doaj.org/article/e5f04cf03aaf4e58ad763101612d0b60 |
op_doi |
https://doi.org/10.1186/1475-2875-11-265 |
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Malaria Journal |
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11 |
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1766345045687926784 |