Gene expression patterns in larval Schistosoma mansoni associated with infection of the mammalian host.
The infective schistosome cercaria develops within the intramolluscan daughter sporocyst from an undifferentiated germ ball, during which synthesis of proteins essential for infection occurs. When the aquatic cercaria locates the mammalian host it rapidly penetrates into the epidermis using glandula...
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ftdoajarticles:oai:doaj.org/article:e5d8acbef1564b978b98b9f6a1d5cb70 2023-05-15T15:16:08+02:00 Gene expression patterns in larval Schistosoma mansoni associated with infection of the mammalian host. Sophia J Parker-Manuel Alasdair C Ivens Gary P Dillon R Alan Wilson 2011-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001274 https://doaj.org/article/e5d8acbef1564b978b98b9f6a1d5cb70 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3166049?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001274 https://doaj.org/article/e5d8acbef1564b978b98b9f6a1d5cb70 PLoS Neglected Tropical Diseases, Vol 5, Iss 8, p e1274 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001274 2022-12-31T03:44:30Z The infective schistosome cercaria develops within the intramolluscan daughter sporocyst from an undifferentiated germ ball, during which synthesis of proteins essential for infection occurs. When the aquatic cercaria locates the mammalian host it rapidly penetrates into the epidermis using glandular secretions. It then undergoes metamorphosis into the schistosomulum, including replacement of its tegument surface membranes, a process taking several days before it exits the skin. Patterns of gene expression underlying this transition have been characterised.All gene models from the S. mansoni genome (www.GeneDB.org) were incorporated into a high-density oligonucleotide array. Double-stranded cDNA from germ balls, cercariae, and day 3 schistosomula was hybridised to the array without amplification. Statistical analysis was performed using Bioconductor to reveal differentially transcribed loci. Genes were categorised on the basis of biological process, tissue association or molecular function to aid understanding of the complex processes occurring. Genes necessary for DNA replication were enriched only in the germ ball, while those involved in translation were up-regulated in the germ ball and/or day 3 schistosomulum. Different sets of developmental genes were up-regulated at each stage. A large number of genes encoding elastases and invadolysins, and some venom allergen-like proteins were up-regulated in the germ ball, those encoding cysteine and aspartic proteases in the cercaria and schistosomulum. Micro exon genes encoding variant secreted proteins were highly up-regulated in the schistosomulum along with tegument and gut-associated genes, coincident with remodelling of the parasite body. Genes encoding membrane proteins were prominently up-regulated in the cercaria and/or day 3 schistosomulum.Our study highlights an expanded number of transcripts encoding proteins potentially involved in skin invasion. It illuminates the process of metamorphosis into the schistosomulum and highlights the very early activation ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 8 e1274 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sophia J Parker-Manuel Alasdair C Ivens Gary P Dillon R Alan Wilson Gene expression patterns in larval Schistosoma mansoni associated with infection of the mammalian host. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The infective schistosome cercaria develops within the intramolluscan daughter sporocyst from an undifferentiated germ ball, during which synthesis of proteins essential for infection occurs. When the aquatic cercaria locates the mammalian host it rapidly penetrates into the epidermis using glandular secretions. It then undergoes metamorphosis into the schistosomulum, including replacement of its tegument surface membranes, a process taking several days before it exits the skin. Patterns of gene expression underlying this transition have been characterised.All gene models from the S. mansoni genome (www.GeneDB.org) were incorporated into a high-density oligonucleotide array. Double-stranded cDNA from germ balls, cercariae, and day 3 schistosomula was hybridised to the array without amplification. Statistical analysis was performed using Bioconductor to reveal differentially transcribed loci. Genes were categorised on the basis of biological process, tissue association or molecular function to aid understanding of the complex processes occurring. Genes necessary for DNA replication were enriched only in the germ ball, while those involved in translation were up-regulated in the germ ball and/or day 3 schistosomulum. Different sets of developmental genes were up-regulated at each stage. A large number of genes encoding elastases and invadolysins, and some venom allergen-like proteins were up-regulated in the germ ball, those encoding cysteine and aspartic proteases in the cercaria and schistosomulum. Micro exon genes encoding variant secreted proteins were highly up-regulated in the schistosomulum along with tegument and gut-associated genes, coincident with remodelling of the parasite body. Genes encoding membrane proteins were prominently up-regulated in the cercaria and/or day 3 schistosomulum.Our study highlights an expanded number of transcripts encoding proteins potentially involved in skin invasion. It illuminates the process of metamorphosis into the schistosomulum and highlights the very early activation ... |
format |
Article in Journal/Newspaper |
author |
Sophia J Parker-Manuel Alasdair C Ivens Gary P Dillon R Alan Wilson |
author_facet |
Sophia J Parker-Manuel Alasdair C Ivens Gary P Dillon R Alan Wilson |
author_sort |
Sophia J Parker-Manuel |
title |
Gene expression patterns in larval Schistosoma mansoni associated with infection of the mammalian host. |
title_short |
Gene expression patterns in larval Schistosoma mansoni associated with infection of the mammalian host. |
title_full |
Gene expression patterns in larval Schistosoma mansoni associated with infection of the mammalian host. |
title_fullStr |
Gene expression patterns in larval Schistosoma mansoni associated with infection of the mammalian host. |
title_full_unstemmed |
Gene expression patterns in larval Schistosoma mansoni associated with infection of the mammalian host. |
title_sort |
gene expression patterns in larval schistosoma mansoni associated with infection of the mammalian host. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001274 https://doaj.org/article/e5d8acbef1564b978b98b9f6a1d5cb70 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 8, p e1274 (2011) |
op_relation |
http://europepmc.org/articles/PMC3166049?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001274 https://doaj.org/article/e5d8acbef1564b978b98b9f6a1d5cb70 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001274 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
8 |
container_start_page |
e1274 |
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1766346436104945664 |