Lectins for the detection of IgM antibodies to T. gondii in the diagnosis of acute toxoplasmosis by immunofluorescence test
Lectins were labeled with fluorescein and tried as conjugates in the immunofluorescence (IP) test for the detection of IgM antibodies to T. gondii, in the diagnosis of acute toxoplasmosis. This approach was an attempt to find alternative reagents for anti-human IgM fluorescent conjugates (AHIgMFC),...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo
1987
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ftdoajarticles:oai:doaj.org/article:e5bdb63515854bdd83413cf817d97b65 2023-05-15T15:05:13+02:00 Lectins for the detection of IgM antibodies to T. gondii in the diagnosis of acute toxoplasmosis by immunofluorescence test I. T. I. Nojimoto S. Hoshino-Shimizu T. K. Nagasse-Sugahara M. E. Camargo 1987-12-01T00:00:00Z https://doi.org/10.1590/S0036-46651987000600004 https://doaj.org/article/e5bdb63515854bdd83413cf817d97b65 EN PT eng por Universidade de São Paulo http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651987000600004&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651987000600004 https://doaj.org/article/e5bdb63515854bdd83413cf817d97b65 Revista do Instituto de Medicina Tropical de São Paulo, Vol 29, Iss 6, Pp 354-360 (1987) Anti-IgM detection Lectins Acute toxoplasmosis Lectin conjugate Arctic medicine. Tropical medicine RC955-962 article 1987 ftdoajarticles https://doi.org/10.1590/S0036-46651987000600004 2023-01-08T01:32:36Z Lectins were labeled with fluorescein and tried as conjugates in the immunofluorescence (IP) test for the detection of IgM antibodies to T. gondii, in the diagnosis of acute toxoplasmosis. This approach was an attempt to find alternative reagents for anti-human IgM fluorescent conjugates (AHIgMFC), which contain quite frequently anaibcdies to toxoplasma, as contaminants, due to natural T. gondii infections among animals used for imunization. Lentil (Lens culinaris) lectin fluorescence conjugates (LcFC) provided most satisfactory results. The evaluation of LcFC carried out in a total of 179 sera from patients with acute and chronic toxoplasmosis, with non-related infections or healthy subjects, gave high values of relative efficiency, co-positivity and co-negativity indices, respectively 0.989, 0.969 and 1.000, in reference to the conventional AHIgMFC. Moreover, three batches of LcFC successively prepared gave reproducible test results. The advantage of LcFC as an alternative reagent for the serodiagnosis of acute toxoplasmosis is supported by practical aspects of its preparation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 29 6 354 360 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Portuguese |
topic |
Anti-IgM detection Lectins Acute toxoplasmosis Lectin conjugate Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Anti-IgM detection Lectins Acute toxoplasmosis Lectin conjugate Arctic medicine. Tropical medicine RC955-962 I. T. I. Nojimoto S. Hoshino-Shimizu T. K. Nagasse-Sugahara M. E. Camargo Lectins for the detection of IgM antibodies to T. gondii in the diagnosis of acute toxoplasmosis by immunofluorescence test |
topic_facet |
Anti-IgM detection Lectins Acute toxoplasmosis Lectin conjugate Arctic medicine. Tropical medicine RC955-962 |
description |
Lectins were labeled with fluorescein and tried as conjugates in the immunofluorescence (IP) test for the detection of IgM antibodies to T. gondii, in the diagnosis of acute toxoplasmosis. This approach was an attempt to find alternative reagents for anti-human IgM fluorescent conjugates (AHIgMFC), which contain quite frequently anaibcdies to toxoplasma, as contaminants, due to natural T. gondii infections among animals used for imunization. Lentil (Lens culinaris) lectin fluorescence conjugates (LcFC) provided most satisfactory results. The evaluation of LcFC carried out in a total of 179 sera from patients with acute and chronic toxoplasmosis, with non-related infections or healthy subjects, gave high values of relative efficiency, co-positivity and co-negativity indices, respectively 0.989, 0.969 and 1.000, in reference to the conventional AHIgMFC. Moreover, three batches of LcFC successively prepared gave reproducible test results. The advantage of LcFC as an alternative reagent for the serodiagnosis of acute toxoplasmosis is supported by practical aspects of its preparation. |
format |
Article in Journal/Newspaper |
author |
I. T. I. Nojimoto S. Hoshino-Shimizu T. K. Nagasse-Sugahara M. E. Camargo |
author_facet |
I. T. I. Nojimoto S. Hoshino-Shimizu T. K. Nagasse-Sugahara M. E. Camargo |
author_sort |
I. T. I. Nojimoto |
title |
Lectins for the detection of IgM antibodies to T. gondii in the diagnosis of acute toxoplasmosis by immunofluorescence test |
title_short |
Lectins for the detection of IgM antibodies to T. gondii in the diagnosis of acute toxoplasmosis by immunofluorescence test |
title_full |
Lectins for the detection of IgM antibodies to T. gondii in the diagnosis of acute toxoplasmosis by immunofluorescence test |
title_fullStr |
Lectins for the detection of IgM antibodies to T. gondii in the diagnosis of acute toxoplasmosis by immunofluorescence test |
title_full_unstemmed |
Lectins for the detection of IgM antibodies to T. gondii in the diagnosis of acute toxoplasmosis by immunofluorescence test |
title_sort |
lectins for the detection of igm antibodies to t. gondii in the diagnosis of acute toxoplasmosis by immunofluorescence test |
publisher |
Universidade de São Paulo |
publishDate |
1987 |
url |
https://doi.org/10.1590/S0036-46651987000600004 https://doaj.org/article/e5bdb63515854bdd83413cf817d97b65 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 29, Iss 6, Pp 354-360 (1987) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651987000600004&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651987000600004 https://doaj.org/article/e5bdb63515854bdd83413cf817d97b65 |
op_doi |
https://doi.org/10.1590/S0036-46651987000600004 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
29 |
container_issue |
6 |
container_start_page |
354 |
op_container_end_page |
360 |
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