Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract
Objective: To evaluate the cytotoxic activity of wood extracts of Lunasia amara Blanco (L. amara) and to perform further phytochemical standardization. Methods: The wood extracts of L. amara were assessed for cytotoxic activity by in vitro tetrazolium bromide (MTT) method against two human cancer ce...
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Wolters Kluwer Medknow Publications
2016
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ftdoajarticles:oai:doaj.org/article:e555450ebc4c479f8a5c65e144f7314a 2023-05-15T15:07:16+02:00 Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract Muhammad Sulaiman Zubair Syariful Anam Subehan Lallo 2016-11-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.04.014 https://doaj.org/article/e555450ebc4c479f8a5c65e144f7314a EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116307985 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.04.014 https://doaj.org/article/e555450ebc4c479f8a5c65e144f7314a Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 11, Pp 962-966 (2016) Lunasia amara Anticancer HeLa T47D Total alkaloid Lunacrine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.04.014 2022-12-31T14:08:06Z Objective: To evaluate the cytotoxic activity of wood extracts of Lunasia amara Blanco (L. amara) and to perform further phytochemical standardization. Methods: The wood extracts of L. amara were assessed for cytotoxic activity by in vitro tetrazolium bromide (MTT) method against two human cancer cell lines, cervical cancer cells (HeLa) and breast cancer cells (T47D). Thin layer chromatography, Dragendorf, acetic anhydride-sulfuric acid and ferric chloride were used to detect alkaloids, steroids and polyphenols, respectively. Furthermore, quantitative determination of total alkaloid by ultra fast liquid chromatography-photodiode array detection using lunacrine as a marker compound was performed as well. Results: The ethyl acetate extract exhibited higher cell-growth inhibition than methanol and n-hexane extracts on HeLa and T47D cancer line cells with the IC50 of 71.15 and 79.04 μg/mL, respectively. Total alkaloid in ethyl acetate extract was counted as (10.46 ± 0.28)% (w/w), while lunacrine determined by ultra fast liquid chromatography-photodiode array detection method was found to be (3.55 ± 0.26)% (w/w). Conclusions: The high total alkaloid and lunacrine concentration on the extract confirm the potential cytotoxic property of ethyl acetate wood extract of L. amara. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Blanco ENVELOPE(-55.233,-55.233,-61.250,-61.250) Asian Pacific Journal of Tropical Biomedicine 6 11 962 966 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Lunasia amara Anticancer HeLa T47D Total alkaloid Lunacrine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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Lunasia amara Anticancer HeLa T47D Total alkaloid Lunacrine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Muhammad Sulaiman Zubair Syariful Anam Subehan Lallo Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract |
topic_facet |
Lunasia amara Anticancer HeLa T47D Total alkaloid Lunacrine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the cytotoxic activity of wood extracts of Lunasia amara Blanco (L. amara) and to perform further phytochemical standardization. Methods: The wood extracts of L. amara were assessed for cytotoxic activity by in vitro tetrazolium bromide (MTT) method against two human cancer cell lines, cervical cancer cells (HeLa) and breast cancer cells (T47D). Thin layer chromatography, Dragendorf, acetic anhydride-sulfuric acid and ferric chloride were used to detect alkaloids, steroids and polyphenols, respectively. Furthermore, quantitative determination of total alkaloid by ultra fast liquid chromatography-photodiode array detection using lunacrine as a marker compound was performed as well. Results: The ethyl acetate extract exhibited higher cell-growth inhibition than methanol and n-hexane extracts on HeLa and T47D cancer line cells with the IC50 of 71.15 and 79.04 μg/mL, respectively. Total alkaloid in ethyl acetate extract was counted as (10.46 ± 0.28)% (w/w), while lunacrine determined by ultra fast liquid chromatography-photodiode array detection method was found to be (3.55 ± 0.26)% (w/w). Conclusions: The high total alkaloid and lunacrine concentration on the extract confirm the potential cytotoxic property of ethyl acetate wood extract of L. amara. |
format |
Article in Journal/Newspaper |
author |
Muhammad Sulaiman Zubair Syariful Anam Subehan Lallo |
author_facet |
Muhammad Sulaiman Zubair Syariful Anam Subehan Lallo |
author_sort |
Muhammad Sulaiman Zubair |
title |
Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract |
title_short |
Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract |
title_full |
Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract |
title_fullStr |
Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract |
title_full_unstemmed |
Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract |
title_sort |
cytotoxic activity and phytochemical standardization of lunasia amara blanco wood extract |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2016.04.014 https://doaj.org/article/e555450ebc4c479f8a5c65e144f7314a |
long_lat |
ENVELOPE(-55.233,-55.233,-61.250,-61.250) |
geographic |
Arctic Blanco |
geographic_facet |
Arctic Blanco |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 11, Pp 962-966 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116307985 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.04.014 https://doaj.org/article/e555450ebc4c479f8a5c65e144f7314a |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.04.014 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
11 |
container_start_page |
962 |
op_container_end_page |
966 |
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1766338807044505600 |