Effects of Ocean Acidification on Molting, Oxidative Stress, and Gut Microbiota in Juvenile Horseshoe Crab Tachypleus tridentatus
Anthropogenic elevation of atmospheric carbon dioxide (CO2) drives global-scale ocean acidification (OA), which has aroused widespread concern for marine ecosystem health. The tri-spine horseshoe crab (HSC) Tachypleus tridentatus has been facing the threat of population depletion for decades, and th...
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ftdoajarticles:oai:doaj.org/article:e537c49c88014411b620b32cd974d962 2023-05-15T17:50:19+02:00 Effects of Ocean Acidification on Molting, Oxidative Stress, and Gut Microbiota in Juvenile Horseshoe Crab Tachypleus tridentatus Ximei Liu Jiani Liu Kai Xiong Caoqi Zhang James Kar-Hei Fang Jie Song Zongguang Tai Quangang Zhu Menghong Hu Youji Wang 2022-01-01T00:00:00Z https://doi.org/10.3389/fphys.2021.813582 https://doaj.org/article/e537c49c88014411b620b32cd974d962 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fphys.2021.813582/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2021.813582 https://doaj.org/article/e537c49c88014411b620b32cd974d962 Frontiers in Physiology, Vol 12 (2022) ocean acidification horseshoe crab molting ecdysone oxidative stress gut microbiota Physiology QP1-981 article 2022 ftdoajarticles https://doi.org/10.3389/fphys.2021.813582 2022-12-31T16:25:43Z Anthropogenic elevation of atmospheric carbon dioxide (CO2) drives global-scale ocean acidification (OA), which has aroused widespread concern for marine ecosystem health. The tri-spine horseshoe crab (HSC) Tachypleus tridentatus has been facing the threat of population depletion for decades, and the effects of OA on the physiology and microbiology of its early life stage are unclear. In this study, the 1st instar HSC larvae were exposed to acidified seawater (pH 7.3, pH 8.1 as control) for 28 days to determine the effects of OA on their growth, molting, oxidative stress, and gut microbiota. Results showed that there were no significant differences in growth index and molting rate between OA group and control group, but the chitinase activity, β-NAGase activity, and ecdysone content in OA group were significantly lower than those of the control group. Compared to the control group, reactive oxygen species (ROS) and malondialdehyde (MDA) contents in OA group were significantly increased at the end of the experiment. Superoxide dismutase (SOD), catalase (CAT), and alkaline phosphatase (AKP) activities increased first and then decreased, glutathione peroxidase (GPX) decreased first and then increased, and GST activity changed little during the experiment. According to the result of 16S rRNA sequencing of gut microbiota, microbial-mediated functions predicted by PICRUSt showed that “Hematopoietic cell lineage,” “Endocytosis,” “Staphylococcus aureus infection,” and “Shigellosis” pathways significantly increased in OA group. The above results indicate that OA had no significant effect on growth index and molting rate but interfered with the activity of chitinolytic enzymes and ecdysone expression of juvenile horseshoe crabs, and caused oxidative stress. In addition, OA had adverse effects on the immune defense function and intestinal health. The present study reveals the potential threat of OA to T. tridentatus population and lays a foundation for the further study of the physiological adaptation mechanism of juvenile ... Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles Frontiers in Physiology 12 |
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topic |
ocean acidification horseshoe crab molting ecdysone oxidative stress gut microbiota Physiology QP1-981 |
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ocean acidification horseshoe crab molting ecdysone oxidative stress gut microbiota Physiology QP1-981 Ximei Liu Jiani Liu Kai Xiong Caoqi Zhang James Kar-Hei Fang Jie Song Zongguang Tai Quangang Zhu Menghong Hu Youji Wang Effects of Ocean Acidification on Molting, Oxidative Stress, and Gut Microbiota in Juvenile Horseshoe Crab Tachypleus tridentatus |
topic_facet |
ocean acidification horseshoe crab molting ecdysone oxidative stress gut microbiota Physiology QP1-981 |
description |
Anthropogenic elevation of atmospheric carbon dioxide (CO2) drives global-scale ocean acidification (OA), which has aroused widespread concern for marine ecosystem health. The tri-spine horseshoe crab (HSC) Tachypleus tridentatus has been facing the threat of population depletion for decades, and the effects of OA on the physiology and microbiology of its early life stage are unclear. In this study, the 1st instar HSC larvae were exposed to acidified seawater (pH 7.3, pH 8.1 as control) for 28 days to determine the effects of OA on their growth, molting, oxidative stress, and gut microbiota. Results showed that there were no significant differences in growth index and molting rate between OA group and control group, but the chitinase activity, β-NAGase activity, and ecdysone content in OA group were significantly lower than those of the control group. Compared to the control group, reactive oxygen species (ROS) and malondialdehyde (MDA) contents in OA group were significantly increased at the end of the experiment. Superoxide dismutase (SOD), catalase (CAT), and alkaline phosphatase (AKP) activities increased first and then decreased, glutathione peroxidase (GPX) decreased first and then increased, and GST activity changed little during the experiment. According to the result of 16S rRNA sequencing of gut microbiota, microbial-mediated functions predicted by PICRUSt showed that “Hematopoietic cell lineage,” “Endocytosis,” “Staphylococcus aureus infection,” and “Shigellosis” pathways significantly increased in OA group. The above results indicate that OA had no significant effect on growth index and molting rate but interfered with the activity of chitinolytic enzymes and ecdysone expression of juvenile horseshoe crabs, and caused oxidative stress. In addition, OA had adverse effects on the immune defense function and intestinal health. The present study reveals the potential threat of OA to T. tridentatus population and lays a foundation for the further study of the physiological adaptation mechanism of juvenile ... |
format |
Article in Journal/Newspaper |
author |
Ximei Liu Jiani Liu Kai Xiong Caoqi Zhang James Kar-Hei Fang Jie Song Zongguang Tai Quangang Zhu Menghong Hu Youji Wang |
author_facet |
Ximei Liu Jiani Liu Kai Xiong Caoqi Zhang James Kar-Hei Fang Jie Song Zongguang Tai Quangang Zhu Menghong Hu Youji Wang |
author_sort |
Ximei Liu |
title |
Effects of Ocean Acidification on Molting, Oxidative Stress, and Gut Microbiota in Juvenile Horseshoe Crab Tachypleus tridentatus |
title_short |
Effects of Ocean Acidification on Molting, Oxidative Stress, and Gut Microbiota in Juvenile Horseshoe Crab Tachypleus tridentatus |
title_full |
Effects of Ocean Acidification on Molting, Oxidative Stress, and Gut Microbiota in Juvenile Horseshoe Crab Tachypleus tridentatus |
title_fullStr |
Effects of Ocean Acidification on Molting, Oxidative Stress, and Gut Microbiota in Juvenile Horseshoe Crab Tachypleus tridentatus |
title_full_unstemmed |
Effects of Ocean Acidification on Molting, Oxidative Stress, and Gut Microbiota in Juvenile Horseshoe Crab Tachypleus tridentatus |
title_sort |
effects of ocean acidification on molting, oxidative stress, and gut microbiota in juvenile horseshoe crab tachypleus tridentatus |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fphys.2021.813582 https://doaj.org/article/e537c49c88014411b620b32cd974d962 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Frontiers in Physiology, Vol 12 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fphys.2021.813582/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2021.813582 https://doaj.org/article/e537c49c88014411b620b32cd974d962 |
op_doi |
https://doi.org/10.3389/fphys.2021.813582 |
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Frontiers in Physiology |
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12 |
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1766157026737520640 |