Nuevas evidencias sobre la utilidad diagnóstica de una fórmula no comercial para la detección de la actividad de ureasa de Helicobacter pylori en biopsias gástricas

• Main Authors: Fuenmayor B Alisbeth, Hernández R Ileana, Paz M América, Cavazza María E, Lizarzábal G,Maribel
• Format: Article in Journal/Newspaper
• Language: English; Spanish
• Published: Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales 2006
• Subjects: Helicobacter pylori; diagnosis; urease test; gastric biopsies; gastrointestinal endoscopy; diagnóstico; prueba de ureasa; biopsia gástrica; endoscopia gastrointestinal; Arctic medicine. Tropical medicine; RC955-962; Public aspects of medicine; RA1-1270; Arctic; Christensen; Elevado
• Online Access: https://doaj.org/article/e52f98fb166a47b6a8516f7283f7a499

Abstract: The role of Helicobacter pylori on the development of gastro-duodenal diseases and gastric cancer, demands the availability of reliable tests to diagnose that infectious disease. The rapid urease test, widely used, is a valuable tool for this purpose, but its expense limits the applicability of this test in our country. In this research, the diagnostic validity of a non-commercial inexpensive urease test was evaluated. It was prepared with Christensen’s urea agar, applied to antral gastric biopsies from 75 symptomatic adults who were submitted to digestive endoscopy. The positive result to any of the following methods was used as infection criteria: bacteriologic culture, Giemsa histological stain and polymerase chain reaction 35 out of 37 patients, in whom the infection was detected, were correctly diagnosed by this test (94.6%), and 29 of them (82.8%) were detected after two-hour incubation. The rate of false negatives was significantly reduced, and both sensitivity and diagnostic accuracy were increased in the readings practiced after 24-hour incubation. In conclusion, the performance of the urease test evaluated suggests that this is a highly reliable and low-cost tool for the diagnosis of H. pylori infection in individuals submitted to digestive endoscopy. Resumen: La asociación entre la infección por Helicobacter pylori y el desarrollo de enfermedades gastroduodenales y cáncer gástrico, exige disponer de pruebas confiables para diagnosticar esta infección. La prueba rápida de ureasa, ampliamente difundida, es una herramienta valiosa para este fin, pero su elevado costo limita su aplicabilidad en nuestro medio. En el presente trabajo se evaluó la validez diagnóstica de una prueba de ureasa no comercial y de bajo costo, preparada con agar urea de Christensen, aplicada a biopsias gástricas antrales de 75 adultos sintomáticos, sometidos a endoscopia gastrointestinal. Como criterio de infección, se empleó la positividad a cualquiera de las siguientes metodologías: cultivo bacteriológico, coloración ...