Ontogeny of the B- and T-cell response in a primary Zika virus infection of a dengue-naïve individual during the 2016 outbreak in Miami, FL.
Zika virus (ZIKV) is a mosquito-borne flavivirus of significant public health concern. In the summer of 2016, ZIKV was first detected in the contiguous United States. Here we present one of the first cases of a locally acquired ZIKV infection in a dengue-naïve individual. We collected blood from a f...
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ftdoajarticles:oai:doaj.org/article:e517563d855b415c83c79c2d54bdefb8 2023-05-15T15:14:50+02:00 Ontogeny of the B- and T-cell response in a primary Zika virus infection of a dengue-naïve individual during the 2016 outbreak in Miami, FL. Michael J Ricciardi Diogo M Magnani Alba Grifoni Young-Chan Kwon Martin J Gutman Nathan D Grubaugh Karthik Gangavarapu Mark Sharkey Cassia G T Silveira Varian K Bailey Núria Pedreño-Lopez Lucas Gonzalez-Nieto Helen S Maxwell Aline Domingues Mauricio A Martins John Pham Daniela Weiskopf John Altman Esper G Kallas Kristian G Andersen Mario Stevenson Paola Lichtenberger Hyeryun Choe Stephen S Whitehead Alessandro Sette David I Watkins 2017-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006000 https://doaj.org/article/e517563d855b415c83c79c2d54bdefb8 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5755934?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006000 https://doaj.org/article/e517563d855b415c83c79c2d54bdefb8 PLoS Neglected Tropical Diseases, Vol 11, Iss 12, p e0006000 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006000 2022-12-31T03:35:52Z Zika virus (ZIKV) is a mosquito-borne flavivirus of significant public health concern. In the summer of 2016, ZIKV was first detected in the contiguous United States. Here we present one of the first cases of a locally acquired ZIKV infection in a dengue-naïve individual. We collected blood from a female with a maculopapular rash at day (D) 5 and D7 post onset of symptoms (POS) and we continued weekly blood draws out to D148 POS. To establish the ontogeny of the immune response against ZIKV, lymphocytes and plasma were analyzed in a longitudinal fashion. The plasmablast response peaked at D7 POS (19.6% of CD19+ B-cells) and was undetectable by D15 POS. ZIKV-specific IgM was present at D5 POS, peaked between D15 and D21 POS, and subsequently decreased. The ZIKV-specific IgG response, however, was not detected until D15 POS and continued to increase after that. Interestingly, even though the patient had never been infected with dengue virus (DENV), cross-reactive IgM and IgG binding against each of the four DENV serotypes could be detected. The highest plasma neutralization activity against ZIKV peaked between D15 and D21 POS, and even though DENV binding antibodies were present in the plasma of the patient, there was neither neutralization nor antibody dependent enhancement (ADE) of DENV. Interestingly, ADE against ZIKV arose at D48 POS and continued until the end of the study. CD4+ and CD8+ T-cells recognized ZIKV-NS2A and ZIKV-E, respectively. The tetramer positive CD8+ T-cell response peaked at D21 POS with elevated levels persisting for months. In summary, this is the first study to establish the timing of the ontogeny of the immune response against ZIKV. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 12 e0006000 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Michael J Ricciardi Diogo M Magnani Alba Grifoni Young-Chan Kwon Martin J Gutman Nathan D Grubaugh Karthik Gangavarapu Mark Sharkey Cassia G T Silveira Varian K Bailey Núria Pedreño-Lopez Lucas Gonzalez-Nieto Helen S Maxwell Aline Domingues Mauricio A Martins John Pham Daniela Weiskopf John Altman Esper G Kallas Kristian G Andersen Mario Stevenson Paola Lichtenberger Hyeryun Choe Stephen S Whitehead Alessandro Sette David I Watkins Ontogeny of the B- and T-cell response in a primary Zika virus infection of a dengue-naïve individual during the 2016 outbreak in Miami, FL. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Zika virus (ZIKV) is a mosquito-borne flavivirus of significant public health concern. In the summer of 2016, ZIKV was first detected in the contiguous United States. Here we present one of the first cases of a locally acquired ZIKV infection in a dengue-naïve individual. We collected blood from a female with a maculopapular rash at day (D) 5 and D7 post onset of symptoms (POS) and we continued weekly blood draws out to D148 POS. To establish the ontogeny of the immune response against ZIKV, lymphocytes and plasma were analyzed in a longitudinal fashion. The plasmablast response peaked at D7 POS (19.6% of CD19+ B-cells) and was undetectable by D15 POS. ZIKV-specific IgM was present at D5 POS, peaked between D15 and D21 POS, and subsequently decreased. The ZIKV-specific IgG response, however, was not detected until D15 POS and continued to increase after that. Interestingly, even though the patient had never been infected with dengue virus (DENV), cross-reactive IgM and IgG binding against each of the four DENV serotypes could be detected. The highest plasma neutralization activity against ZIKV peaked between D15 and D21 POS, and even though DENV binding antibodies were present in the plasma of the patient, there was neither neutralization nor antibody dependent enhancement (ADE) of DENV. Interestingly, ADE against ZIKV arose at D48 POS and continued until the end of the study. CD4+ and CD8+ T-cells recognized ZIKV-NS2A and ZIKV-E, respectively. The tetramer positive CD8+ T-cell response peaked at D21 POS with elevated levels persisting for months. In summary, this is the first study to establish the timing of the ontogeny of the immune response against ZIKV. |
format |
Article in Journal/Newspaper |
author |
Michael J Ricciardi Diogo M Magnani Alba Grifoni Young-Chan Kwon Martin J Gutman Nathan D Grubaugh Karthik Gangavarapu Mark Sharkey Cassia G T Silveira Varian K Bailey Núria Pedreño-Lopez Lucas Gonzalez-Nieto Helen S Maxwell Aline Domingues Mauricio A Martins John Pham Daniela Weiskopf John Altman Esper G Kallas Kristian G Andersen Mario Stevenson Paola Lichtenberger Hyeryun Choe Stephen S Whitehead Alessandro Sette David I Watkins |
author_facet |
Michael J Ricciardi Diogo M Magnani Alba Grifoni Young-Chan Kwon Martin J Gutman Nathan D Grubaugh Karthik Gangavarapu Mark Sharkey Cassia G T Silveira Varian K Bailey Núria Pedreño-Lopez Lucas Gonzalez-Nieto Helen S Maxwell Aline Domingues Mauricio A Martins John Pham Daniela Weiskopf John Altman Esper G Kallas Kristian G Andersen Mario Stevenson Paola Lichtenberger Hyeryun Choe Stephen S Whitehead Alessandro Sette David I Watkins |
author_sort |
Michael J Ricciardi |
title |
Ontogeny of the B- and T-cell response in a primary Zika virus infection of a dengue-naïve individual during the 2016 outbreak in Miami, FL. |
title_short |
Ontogeny of the B- and T-cell response in a primary Zika virus infection of a dengue-naïve individual during the 2016 outbreak in Miami, FL. |
title_full |
Ontogeny of the B- and T-cell response in a primary Zika virus infection of a dengue-naïve individual during the 2016 outbreak in Miami, FL. |
title_fullStr |
Ontogeny of the B- and T-cell response in a primary Zika virus infection of a dengue-naïve individual during the 2016 outbreak in Miami, FL. |
title_full_unstemmed |
Ontogeny of the B- and T-cell response in a primary Zika virus infection of a dengue-naïve individual during the 2016 outbreak in Miami, FL. |
title_sort |
ontogeny of the b- and t-cell response in a primary zika virus infection of a dengue-naïve individual during the 2016 outbreak in miami, fl. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0006000 https://doaj.org/article/e517563d855b415c83c79c2d54bdefb8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 12, p e0006000 (2017) |
op_relation |
http://europepmc.org/articles/PMC5755934?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006000 https://doaj.org/article/e517563d855b415c83c79c2d54bdefb8 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006000 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
12 |
container_start_page |
e0006000 |
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1766345234534367232 |