An ice-binding protein from an Arctic population of American dunegrass, Leymus mollis [version 2; peer review: 3 approved]
Several cold-hardy grasses have been shown to have ice-binding proteins (IBPs) that protect against freeze-thaw injury. Here, we looked for IBP activity in an Alaskan coastal grass, Leymus mollis (Pooidae), that had not previously been examined. Rhizome tissue had strong ice-structuring and ice recr...
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2020
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ftdoajarticles:oai:doaj.org/article:e50049b1021a4e55b0f63a32cc9ae6a1 2023-05-15T15:01:39+02:00 An ice-binding protein from an Arctic population of American dunegrass, Leymus mollis [version 2; peer review: 3 approved] Todd L. Sformo James A. Raymond 2020-08-01T00:00:00Z https://doi.org/10.12688/f1000research.24328.2 https://doaj.org/article/e50049b1021a4e55b0f63a32cc9ae6a1 EN eng F1000 Research Ltd https://f1000research.com/articles/9-648/v2 https://doaj.org/toc/2046-1402 2046-1402 doi:10.12688/f1000research.24328.2 https://doaj.org/article/e50049b1021a4e55b0f63a32cc9ae6a1 F1000Research, Vol 9 (2020) Medicine R Science Q article 2020 ftdoajarticles https://doi.org/10.12688/f1000research.24328.2 2022-12-31T04:13:06Z Several cold-hardy grasses have been shown to have ice-binding proteins (IBPs) that protect against freeze-thaw injury. Here, we looked for IBP activity in an Alaskan coastal grass, Leymus mollis (Pooidae), that had not previously been examined. Rhizome tissue had strong ice-structuring and ice recrystallization inhibiting (IRI) activities, indicating the probable presence of IBPs. The gene sequence of an IBP was obtained. The sequence encoded a 118-amino acid IRI domain composed of eight repeats and that was 80% identical to the IRI domain of the IBP of perennial ryegrass Lolium perenne. The predicted 3D structure of the IRI domain had eight beta-roll coils like those in L. perenne IBP Article in Journal/Newspaper Arctic Arctic Population Directory of Open Access Journals: DOAJ Articles Arctic F1000Research 9 648 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Todd L. Sformo James A. Raymond An ice-binding protein from an Arctic population of American dunegrass, Leymus mollis [version 2; peer review: 3 approved] |
topic_facet |
Medicine R Science Q |
description |
Several cold-hardy grasses have been shown to have ice-binding proteins (IBPs) that protect against freeze-thaw injury. Here, we looked for IBP activity in an Alaskan coastal grass, Leymus mollis (Pooidae), that had not previously been examined. Rhizome tissue had strong ice-structuring and ice recrystallization inhibiting (IRI) activities, indicating the probable presence of IBPs. The gene sequence of an IBP was obtained. The sequence encoded a 118-amino acid IRI domain composed of eight repeats and that was 80% identical to the IRI domain of the IBP of perennial ryegrass Lolium perenne. The predicted 3D structure of the IRI domain had eight beta-roll coils like those in L. perenne IBP |
format |
Article in Journal/Newspaper |
author |
Todd L. Sformo James A. Raymond |
author_facet |
Todd L. Sformo James A. Raymond |
author_sort |
Todd L. Sformo |
title |
An ice-binding protein from an Arctic population of American dunegrass, Leymus mollis [version 2; peer review: 3 approved] |
title_short |
An ice-binding protein from an Arctic population of American dunegrass, Leymus mollis [version 2; peer review: 3 approved] |
title_full |
An ice-binding protein from an Arctic population of American dunegrass, Leymus mollis [version 2; peer review: 3 approved] |
title_fullStr |
An ice-binding protein from an Arctic population of American dunegrass, Leymus mollis [version 2; peer review: 3 approved] |
title_full_unstemmed |
An ice-binding protein from an Arctic population of American dunegrass, Leymus mollis [version 2; peer review: 3 approved] |
title_sort |
ice-binding protein from an arctic population of american dunegrass, leymus mollis [version 2; peer review: 3 approved] |
publisher |
F1000 Research Ltd |
publishDate |
2020 |
url |
https://doi.org/10.12688/f1000research.24328.2 https://doaj.org/article/e50049b1021a4e55b0f63a32cc9ae6a1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic Population |
genre_facet |
Arctic Arctic Population |
op_source |
F1000Research, Vol 9 (2020) |
op_relation |
https://f1000research.com/articles/9-648/v2 https://doaj.org/toc/2046-1402 2046-1402 doi:10.12688/f1000research.24328.2 https://doaj.org/article/e50049b1021a4e55b0f63a32cc9ae6a1 |
op_doi |
https://doi.org/10.12688/f1000research.24328.2 |
container_title |
F1000Research |
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9 |
container_start_page |
648 |
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1766333660740452352 |