Identificación de Aislados de Cryptococcus neoformans Usando Agar Staib sin Creatinina
In mycology, culture is the best way to demonstrate and identify pathogenic fungi. Many kinds of media have been developed and modified, using fungal biochemical properties to recognize them. The most common media are Sabouraud, Malt Agar, Cornmeal agar, Potato-dextrose agar, and Staib agar. Staib a...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2005
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ftdoajarticles:oai:doaj.org/article:e4f76efccd9045ae8fad0c1b273fbe7c 2023-10-01T03:54:08+02:00 Identificación de Aislados de Cryptococcus neoformans Usando Agar Staib sin Creatinina Vanessa Nardelli Celina Pérez Sofía Mata-Essayag María Teresa Colella Arantza Roselló Claudia Hartung de Capriles, María Eugenia Landaeta Carolina Olaizola Sylvia Magaldi 2005-08-01T00:00:00Z https://doaj.org/article/e4f76efccd9045ae8fad0c1b273fbe7c EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://produccioncientificaluz.org/index.php/kasmera/article/view/4755 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/e4f76efccd9045ae8fad0c1b273fbe7c Kasmera, Vol 33, Iss 2, Pp 102-108 (2005) Agar Staib Guizotia abyssinica Cryptococcus neoformans creatinina Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2005 ftdoajarticles 2023-09-03T00:43:22Z In mycology, culture is the best way to demonstrate and identify pathogenic fungi. Many kinds of media have been developed and modified, using fungal biochemical properties to recognize them. The most common media are Sabouraud, Malt Agar, Cornmeal agar, Potato-dextrose agar, and Staib agar. Staib agar has been widely used for identification of yeasts from the genus Cryptococcus and other fungi. The aim of this study was to demonstrate the usefulness of Staib agar, and of a modified Staib medium without creatinine, in the isolation and identification of fungi. Forty-six Cryptococcus neoformans strains plus one control strain were identified by morphological and biochemical criteria, using urease test (Christensen urea agar) and culture in Sablac medium. The strains were then transferred into standard Staib and Staib without creatinine agar plates. Morphological features and phenoloxidase production were evaluated after one week incubation at room temperature (26-28°C). Culture in standard and modified Staib agar yielded brown color colonies (phenoloxidase production) in both media in 43 (91.5%) cases. Staib agar without creatinine is an excellent medium for identification of C. neoformans, with the advantage of a low cost and easy preparation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Christensen ENVELOPE(47.867,47.867,-67.967,-67.967) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
Agar Staib Guizotia abyssinica Cryptococcus neoformans creatinina Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Agar Staib Guizotia abyssinica Cryptococcus neoformans creatinina Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Vanessa Nardelli Celina Pérez Sofía Mata-Essayag María Teresa Colella Arantza Roselló Claudia Hartung de Capriles, María Eugenia Landaeta Carolina Olaizola Sylvia Magaldi Identificación de Aislados de Cryptococcus neoformans Usando Agar Staib sin Creatinina |
topic_facet |
Agar Staib Guizotia abyssinica Cryptococcus neoformans creatinina Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
In mycology, culture is the best way to demonstrate and identify pathogenic fungi. Many kinds of media have been developed and modified, using fungal biochemical properties to recognize them. The most common media are Sabouraud, Malt Agar, Cornmeal agar, Potato-dextrose agar, and Staib agar. Staib agar has been widely used for identification of yeasts from the genus Cryptococcus and other fungi. The aim of this study was to demonstrate the usefulness of Staib agar, and of a modified Staib medium without creatinine, in the isolation and identification of fungi. Forty-six Cryptococcus neoformans strains plus one control strain were identified by morphological and biochemical criteria, using urease test (Christensen urea agar) and culture in Sablac medium. The strains were then transferred into standard Staib and Staib without creatinine agar plates. Morphological features and phenoloxidase production were evaluated after one week incubation at room temperature (26-28°C). Culture in standard and modified Staib agar yielded brown color colonies (phenoloxidase production) in both media in 43 (91.5%) cases. Staib agar without creatinine is an excellent medium for identification of C. neoformans, with the advantage of a low cost and easy preparation. |
format |
Article in Journal/Newspaper |
author |
Vanessa Nardelli Celina Pérez Sofía Mata-Essayag María Teresa Colella Arantza Roselló Claudia Hartung de Capriles, María Eugenia Landaeta Carolina Olaizola Sylvia Magaldi |
author_facet |
Vanessa Nardelli Celina Pérez Sofía Mata-Essayag María Teresa Colella Arantza Roselló Claudia Hartung de Capriles, María Eugenia Landaeta Carolina Olaizola Sylvia Magaldi |
author_sort |
Vanessa Nardelli |
title |
Identificación de Aislados de Cryptococcus neoformans Usando Agar Staib sin Creatinina |
title_short |
Identificación de Aislados de Cryptococcus neoformans Usando Agar Staib sin Creatinina |
title_full |
Identificación de Aislados de Cryptococcus neoformans Usando Agar Staib sin Creatinina |
title_fullStr |
Identificación de Aislados de Cryptococcus neoformans Usando Agar Staib sin Creatinina |
title_full_unstemmed |
Identificación de Aislados de Cryptococcus neoformans Usando Agar Staib sin Creatinina |
title_sort |
identificación de aislados de cryptococcus neoformans usando agar staib sin creatinina |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2005 |
url |
https://doaj.org/article/e4f76efccd9045ae8fad0c1b273fbe7c |
long_lat |
ENVELOPE(47.867,47.867,-67.967,-67.967) |
geographic |
Arctic Christensen |
geographic_facet |
Arctic Christensen |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 33, Iss 2, Pp 102-108 (2005) |
op_relation |
https://produccioncientificaluz.org/index.php/kasmera/article/view/4755 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/e4f76efccd9045ae8fad0c1b273fbe7c |
_version_ |
1778521488809263104 |