Characteristics of clinical isolates of nontuberculous mycobacteria in Java-Indonesia: A multicenter study.
Background Nontuberculous mycobacterial (NTM) lung infections are a major public health concern. Diagnosis of NTM-pulmonary disease (NTM-PD) is difficult because its clinical, microbiological, and radiological features resemble to those of pulmonary tuberculosis (TB), leading to misdiagnosis. Identi...
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ftdoajarticles:oai:doaj.org/article:e4e5bded822c4c71bd3fc3846b0773c2 2023-05-15T15:16:30+02:00 Characteristics of clinical isolates of nontuberculous mycobacteria in Java-Indonesia: A multicenter study. Leli Saptawati Widana Primaningtyas Paramasari Dirgahayu Yusup Subagio Sutanto Brian Wasita Betty Suryawati Titik Nuryastuti Ari Probandari 2022-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011007 https://doaj.org/article/e4e5bded822c4c71bd3fc3846b0773c2 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011007 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011007 https://doaj.org/article/e4e5bded822c4c71bd3fc3846b0773c2 PLoS Neglected Tropical Diseases, Vol 16, Iss 12, p e0011007 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011007 2023-02-12T01:27:53Z Background Nontuberculous mycobacterial (NTM) lung infections are a major public health concern. Diagnosis of NTM-pulmonary disease (NTM-PD) is difficult because its clinical, microbiological, and radiological features resemble to those of pulmonary tuberculosis (TB), leading to misdiagnosis. Identification at the species level is essential for diagnosis and determination of therapy, which is currently not performed routinely in Indonesian laboratories. Methodology and principal findings From January 2020 to May 2021, 94 NTM isolates were collected from three TB referral centers in Java Province. Species were identified using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). Tests were performed to determine antibiotic susceptibility, biofilm formation ability, sliding motility characteristics, and the ability to adhere to and invade pneumocytes. After identifying the species of all the isolates, we found nine groups of NTMs: M. fortuitum group 51% (48/94), M. abscessus 38.3% (36/94), M. intracellulare 3.1% (3/94), M. neoaurum 2.1% (2/94), M. chelonae 1.1% (1/94), M. gordonae 1.1% (1/94), M. szulgai 1.1% (1/94), M. mucogenicum 1.1% (1/94), and M. arupense 1.1% (1/94). Amikacin was the most effective antibiotic against M. fortuitum group and M. abscessus. The M. fortuitum group was significantly better at forming biofilms than M. abscessus, but both had the same sliding motility capability. The ability of the M. fortuitum group to adhere to and invade pneumocytes was better than that of M. abscessus, with the number isolates of the M. fortuitum group capable of superior adhesion and invasion to that of M. abscessus. Conclusions/significance This study shows that M. fortuitum group and M. abscessus were the most common NTM found in Java, Indonesia. The M. fortuitum group and M. abscessus were the most susceptible to amikacin; therefore, this was the empirical treatment of choice. The ability to form biofilms is directly proportional to the ability to adhere to and invade ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 12 e0011007 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Leli Saptawati Widana Primaningtyas Paramasari Dirgahayu Yusup Subagio Sutanto Brian Wasita Betty Suryawati Titik Nuryastuti Ari Probandari Characteristics of clinical isolates of nontuberculous mycobacteria in Java-Indonesia: A multicenter study. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Nontuberculous mycobacterial (NTM) lung infections are a major public health concern. Diagnosis of NTM-pulmonary disease (NTM-PD) is difficult because its clinical, microbiological, and radiological features resemble to those of pulmonary tuberculosis (TB), leading to misdiagnosis. Identification at the species level is essential for diagnosis and determination of therapy, which is currently not performed routinely in Indonesian laboratories. Methodology and principal findings From January 2020 to May 2021, 94 NTM isolates were collected from three TB referral centers in Java Province. Species were identified using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). Tests were performed to determine antibiotic susceptibility, biofilm formation ability, sliding motility characteristics, and the ability to adhere to and invade pneumocytes. After identifying the species of all the isolates, we found nine groups of NTMs: M. fortuitum group 51% (48/94), M. abscessus 38.3% (36/94), M. intracellulare 3.1% (3/94), M. neoaurum 2.1% (2/94), M. chelonae 1.1% (1/94), M. gordonae 1.1% (1/94), M. szulgai 1.1% (1/94), M. mucogenicum 1.1% (1/94), and M. arupense 1.1% (1/94). Amikacin was the most effective antibiotic against M. fortuitum group and M. abscessus. The M. fortuitum group was significantly better at forming biofilms than M. abscessus, but both had the same sliding motility capability. The ability of the M. fortuitum group to adhere to and invade pneumocytes was better than that of M. abscessus, with the number isolates of the M. fortuitum group capable of superior adhesion and invasion to that of M. abscessus. Conclusions/significance This study shows that M. fortuitum group and M. abscessus were the most common NTM found in Java, Indonesia. The M. fortuitum group and M. abscessus were the most susceptible to amikacin; therefore, this was the empirical treatment of choice. The ability to form biofilms is directly proportional to the ability to adhere to and invade ... |
format |
Article in Journal/Newspaper |
author |
Leli Saptawati Widana Primaningtyas Paramasari Dirgahayu Yusup Subagio Sutanto Brian Wasita Betty Suryawati Titik Nuryastuti Ari Probandari |
author_facet |
Leli Saptawati Widana Primaningtyas Paramasari Dirgahayu Yusup Subagio Sutanto Brian Wasita Betty Suryawati Titik Nuryastuti Ari Probandari |
author_sort |
Leli Saptawati |
title |
Characteristics of clinical isolates of nontuberculous mycobacteria in Java-Indonesia: A multicenter study. |
title_short |
Characteristics of clinical isolates of nontuberculous mycobacteria in Java-Indonesia: A multicenter study. |
title_full |
Characteristics of clinical isolates of nontuberculous mycobacteria in Java-Indonesia: A multicenter study. |
title_fullStr |
Characteristics of clinical isolates of nontuberculous mycobacteria in Java-Indonesia: A multicenter study. |
title_full_unstemmed |
Characteristics of clinical isolates of nontuberculous mycobacteria in Java-Indonesia: A multicenter study. |
title_sort |
characteristics of clinical isolates of nontuberculous mycobacteria in java-indonesia: a multicenter study. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0011007 https://doaj.org/article/e4e5bded822c4c71bd3fc3846b0773c2 |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 12, p e0011007 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011007 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011007 https://doaj.org/article/e4e5bded822c4c71bd3fc3846b0773c2 |
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https://doi.org/10.1371/journal.pntd.0011007 |
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PLOS Neglected Tropical Diseases |
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16 |
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12 |
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e0011007 |
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