Distribution of Abundant and Active Planktonic Ciliates in Coastal and Slope Waters Off New England
Despite their important role of linking microbial and classic marine food webs, data on biogeographical patterns of microbial eukaryotic grazers are limited, and even fewer studies have used molecular tools to assess active (i.e., those expressing genes) community members. Marine ciliate diversity i...
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ftdoajarticles:oai:doaj.org/article:e4a8adda3ad146c59f2ca5acd229bb4d 2023-05-15T17:45:41+02:00 Distribution of Abundant and Active Planktonic Ciliates in Coastal and Slope Waters Off New England Sarah J. Tucker George B. McManus Laura A. Katz Jean-David Grattepanche 2017-11-01T00:00:00Z https://doi.org/10.3389/fmicb.2017.02178 https://doaj.org/article/e4a8adda3ad146c59f2ca5acd229bb4d EN eng Frontiers Media S.A. http://journal.frontiersin.org/article/10.3389/fmicb.2017.02178/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2017.02178 https://doaj.org/article/e4a8adda3ad146c59f2ca5acd229bb4d Frontiers in Microbiology, Vol 8 (2017) RNA:DNA depth ciliate oligotrichia choreotrichia DGGE Microbiology QR1-502 article 2017 ftdoajarticles https://doi.org/10.3389/fmicb.2017.02178 2022-12-31T14:14:21Z Despite their important role of linking microbial and classic marine food webs, data on biogeographical patterns of microbial eukaryotic grazers are limited, and even fewer studies have used molecular tools to assess active (i.e., those expressing genes) community members. Marine ciliate diversity is believed to be greatest at the chlorophyll maximum, where there is an abundance of autotrophic prey, and is often assumed to decline with depth. Here, we assess the abundant (DNA) and active (RNA) marine ciliate communities throughout the water column at two stations off the New England coast (Northwest Atlantic)—a coastal station 43 km from shore (40 m depth) and a slope station 135 km off shore (1,000 m). We analyze ciliate communities using a DNA fingerprinting technique, Denaturing Gradient Gel Electrophoresis (DGGE), which captures patterns of abundant community members. We compare estimates of ciliate communities from SSU-rDNA (abundant) and SSU-rRNA (active) and find complex patterns throughout the water column, including many active lineages below the photic zone. Our analyses reveal (1) a number of widely-distributed taxa that are both abundant and active; (2) considerable heterogeneity in patterns of presence/absence of taxa in offshore samples taken 50 m apart throughout the water column; and (3) three distinct ciliate assemblages based on position from shore and depth. Analysis of active (RNA) taxa uncovers biodiversity hidden to traditional DNA-based approaches (e.g., clone library, rDNA amplicon studies). Article in Journal/Newspaper Northwest Atlantic Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 8 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
RNA:DNA depth ciliate oligotrichia choreotrichia DGGE Microbiology QR1-502 |
spellingShingle |
RNA:DNA depth ciliate oligotrichia choreotrichia DGGE Microbiology QR1-502 Sarah J. Tucker George B. McManus Laura A. Katz Jean-David Grattepanche Distribution of Abundant and Active Planktonic Ciliates in Coastal and Slope Waters Off New England |
topic_facet |
RNA:DNA depth ciliate oligotrichia choreotrichia DGGE Microbiology QR1-502 |
description |
Despite their important role of linking microbial and classic marine food webs, data on biogeographical patterns of microbial eukaryotic grazers are limited, and even fewer studies have used molecular tools to assess active (i.e., those expressing genes) community members. Marine ciliate diversity is believed to be greatest at the chlorophyll maximum, where there is an abundance of autotrophic prey, and is often assumed to decline with depth. Here, we assess the abundant (DNA) and active (RNA) marine ciliate communities throughout the water column at two stations off the New England coast (Northwest Atlantic)—a coastal station 43 km from shore (40 m depth) and a slope station 135 km off shore (1,000 m). We analyze ciliate communities using a DNA fingerprinting technique, Denaturing Gradient Gel Electrophoresis (DGGE), which captures patterns of abundant community members. We compare estimates of ciliate communities from SSU-rDNA (abundant) and SSU-rRNA (active) and find complex patterns throughout the water column, including many active lineages below the photic zone. Our analyses reveal (1) a number of widely-distributed taxa that are both abundant and active; (2) considerable heterogeneity in patterns of presence/absence of taxa in offshore samples taken 50 m apart throughout the water column; and (3) three distinct ciliate assemblages based on position from shore and depth. Analysis of active (RNA) taxa uncovers biodiversity hidden to traditional DNA-based approaches (e.g., clone library, rDNA amplicon studies). |
format |
Article in Journal/Newspaper |
author |
Sarah J. Tucker George B. McManus Laura A. Katz Jean-David Grattepanche |
author_facet |
Sarah J. Tucker George B. McManus Laura A. Katz Jean-David Grattepanche |
author_sort |
Sarah J. Tucker |
title |
Distribution of Abundant and Active Planktonic Ciliates in Coastal and Slope Waters Off New England |
title_short |
Distribution of Abundant and Active Planktonic Ciliates in Coastal and Slope Waters Off New England |
title_full |
Distribution of Abundant and Active Planktonic Ciliates in Coastal and Slope Waters Off New England |
title_fullStr |
Distribution of Abundant and Active Planktonic Ciliates in Coastal and Slope Waters Off New England |
title_full_unstemmed |
Distribution of Abundant and Active Planktonic Ciliates in Coastal and Slope Waters Off New England |
title_sort |
distribution of abundant and active planktonic ciliates in coastal and slope waters off new england |
publisher |
Frontiers Media S.A. |
publishDate |
2017 |
url |
https://doi.org/10.3389/fmicb.2017.02178 https://doaj.org/article/e4a8adda3ad146c59f2ca5acd229bb4d |
genre |
Northwest Atlantic |
genre_facet |
Northwest Atlantic |
op_source |
Frontiers in Microbiology, Vol 8 (2017) |
op_relation |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.02178/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2017.02178 https://doaj.org/article/e4a8adda3ad146c59f2ca5acd229bb4d |
op_doi |
https://doi.org/10.3389/fmicb.2017.02178 |
container_title |
Frontiers in Microbiology |
container_volume |
8 |
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1766148884319436800 |