The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill
The Deepwater Horizon oil spill was one of the largest and deepest oil spills recorded. The wellhead was located at approximately 1500 m below the sea where low temperature and high pressure are key environmental characteristics. Using cells collected 4 months following the Deepwater Horizon oil spi...
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ftdoajarticles:oai:doaj.org/article:e48db9a85c0f407ab0dc40e258906b53 2023-05-15T13:38:04+02:00 The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill Angeliki Marietou Roger Chastain Felix Beulig Alberto Scoma Terry C. Hazen Douglas H. Bartlett 2018-04-01T00:00:00Z https://doi.org/10.3389/fmicb.2018.00808 https://doaj.org/article/e48db9a85c0f407ab0dc40e258906b53 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2018.00808/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2018.00808 https://doaj.org/article/e48db9a85c0f407ab0dc40e258906b53 Frontiers in Microbiology, Vol 9 (2018) high pressure Gulf of Mexico Deepwater Horizon oil spill hydrocarbon-degrading microbes Microbiology QR1-502 article 2018 ftdoajarticles https://doi.org/10.3389/fmicb.2018.00808 2022-12-31T12:27:31Z The Deepwater Horizon oil spill was one of the largest and deepest oil spills recorded. The wellhead was located at approximately 1500 m below the sea where low temperature and high pressure are key environmental characteristics. Using cells collected 4 months following the Deepwater Horizon oil spill at the Gulf of Mexico, we set up Macondo crude oil enrichments at wellhead temperature and different pressures to determine the effect of increasing depth/pressure to the in situ microbial community and their ability to degrade oil. We observed oil degradation under all pressure conditions tested [0.1, 15, and 30 megapascals (MPa)], although oil degradation profiles, cell numbers, and hydrocarbon degradation gene abundances indicated greatest activity at atmospheric pressure. Under all incubations the growth of psychrophilic bacteria was promoted. Bacteria closely related to Oleispira antarctica RB-8 dominated the communities at all pressures. At 30 MPa we observed a shift toward Photobacterium, a genus that includes piezophiles. Alphaproteobacterial members of the Sulfitobacter, previously associated with oil-degradation, were also highly abundant at 0.1 MPa. Our results suggest that pressure acts synergistically with low temperature to slow microbial growth and thus oil degradation in deep-sea environments. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 9 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
high pressure Gulf of Mexico Deepwater Horizon oil spill hydrocarbon-degrading microbes Microbiology QR1-502 |
spellingShingle |
high pressure Gulf of Mexico Deepwater Horizon oil spill hydrocarbon-degrading microbes Microbiology QR1-502 Angeliki Marietou Roger Chastain Felix Beulig Alberto Scoma Terry C. Hazen Douglas H. Bartlett The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill |
topic_facet |
high pressure Gulf of Mexico Deepwater Horizon oil spill hydrocarbon-degrading microbes Microbiology QR1-502 |
description |
The Deepwater Horizon oil spill was one of the largest and deepest oil spills recorded. The wellhead was located at approximately 1500 m below the sea where low temperature and high pressure are key environmental characteristics. Using cells collected 4 months following the Deepwater Horizon oil spill at the Gulf of Mexico, we set up Macondo crude oil enrichments at wellhead temperature and different pressures to determine the effect of increasing depth/pressure to the in situ microbial community and their ability to degrade oil. We observed oil degradation under all pressure conditions tested [0.1, 15, and 30 megapascals (MPa)], although oil degradation profiles, cell numbers, and hydrocarbon degradation gene abundances indicated greatest activity at atmospheric pressure. Under all incubations the growth of psychrophilic bacteria was promoted. Bacteria closely related to Oleispira antarctica RB-8 dominated the communities at all pressures. At 30 MPa we observed a shift toward Photobacterium, a genus that includes piezophiles. Alphaproteobacterial members of the Sulfitobacter, previously associated with oil-degradation, were also highly abundant at 0.1 MPa. Our results suggest that pressure acts synergistically with low temperature to slow microbial growth and thus oil degradation in deep-sea environments. |
format |
Article in Journal/Newspaper |
author |
Angeliki Marietou Roger Chastain Felix Beulig Alberto Scoma Terry C. Hazen Douglas H. Bartlett |
author_facet |
Angeliki Marietou Roger Chastain Felix Beulig Alberto Scoma Terry C. Hazen Douglas H. Bartlett |
author_sort |
Angeliki Marietou |
title |
The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill |
title_short |
The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill |
title_full |
The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill |
title_fullStr |
The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill |
title_full_unstemmed |
The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill |
title_sort |
effect of hydrostatic pressure on enrichments of hydrocarbon degrading microbes from the gulf of mexico following the deepwater horizon oil spill |
publisher |
Frontiers Media S.A. |
publishDate |
2018 |
url |
https://doi.org/10.3389/fmicb.2018.00808 https://doaj.org/article/e48db9a85c0f407ab0dc40e258906b53 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Frontiers in Microbiology, Vol 9 (2018) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmicb.2018.00808/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2018.00808 https://doaj.org/article/e48db9a85c0f407ab0dc40e258906b53 |
op_doi |
https://doi.org/10.3389/fmicb.2018.00808 |
container_title |
Frontiers in Microbiology |
container_volume |
9 |
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1766101088457457664 |