A novel mini-DNA barcoding assay to identify processed fins from internationally protected shark species.
There is a growing need to identify shark products in trade, in part due to the recent listing of five commercially important species on the Appendices of the Convention on International Trade in Endangered Species (CITES; porbeagle, Lamna nasus, oceanic whitetip, Carcharhinus longimanus scalloped h...
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ftdoajarticles:oai:doaj.org/article:e47c0fe3e3474ba99c5a9003ad2c5fb9 2023-05-15T15:53:51+02:00 A novel mini-DNA barcoding assay to identify processed fins from internationally protected shark species. Andrew T Fields Debra L Abercrombie Rowena Eng Kevin Feldheim Demian D Chapman 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0114844 https://doaj.org/article/e47c0fe3e3474ba99c5a9003ad2c5fb9 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4315593?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0114844 https://doaj.org/article/e47c0fe3e3474ba99c5a9003ad2c5fb9 PLoS ONE, Vol 10, Iss 2, p e0114844 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0114844 2022-12-30T21:03:53Z There is a growing need to identify shark products in trade, in part due to the recent listing of five commercially important species on the Appendices of the Convention on International Trade in Endangered Species (CITES; porbeagle, Lamna nasus, oceanic whitetip, Carcharhinus longimanus scalloped hammerhead, Sphyrna lewini, smooth hammerhead, S. zygaena and great hammerhead S. mokarran) in addition to three species listed in the early part of this century (whale, Rhincodon typus, basking, Cetorhinus maximus, and white, Carcharodon carcharias). Shark fins are traded internationally to supply the Asian dried seafood market, in which they are used to make the luxury dish shark fin soup. Shark fins usually enter international trade with their skin still intact and can be identified using morphological characters or standard DNA-barcoding approaches. Once they reach Asia and are traded in this region the skin is removed and they are treated with chemicals that eliminate many key diagnostic characters and degrade their DNA ("processed fins"). Here, we present a validated mini-barcode assay based on partial sequences of the cytochrome oxidase I gene that can reliably identify the processed fins of seven of the eight CITES listed shark species. We also demonstrate that the assay can even frequently identify the species or genus of origin of shark fin soup (31 out of 50 samples). Article in Journal/Newspaper Cetorhinus maximus Lamna nasus Porbeagle Directory of Open Access Journals: DOAJ Articles PLOS ONE 10 2 e0114844 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Medicine R Science Q |
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Medicine R Science Q Andrew T Fields Debra L Abercrombie Rowena Eng Kevin Feldheim Demian D Chapman A novel mini-DNA barcoding assay to identify processed fins from internationally protected shark species. |
topic_facet |
Medicine R Science Q |
description |
There is a growing need to identify shark products in trade, in part due to the recent listing of five commercially important species on the Appendices of the Convention on International Trade in Endangered Species (CITES; porbeagle, Lamna nasus, oceanic whitetip, Carcharhinus longimanus scalloped hammerhead, Sphyrna lewini, smooth hammerhead, S. zygaena and great hammerhead S. mokarran) in addition to three species listed in the early part of this century (whale, Rhincodon typus, basking, Cetorhinus maximus, and white, Carcharodon carcharias). Shark fins are traded internationally to supply the Asian dried seafood market, in which they are used to make the luxury dish shark fin soup. Shark fins usually enter international trade with their skin still intact and can be identified using morphological characters or standard DNA-barcoding approaches. Once they reach Asia and are traded in this region the skin is removed and they are treated with chemicals that eliminate many key diagnostic characters and degrade their DNA ("processed fins"). Here, we present a validated mini-barcode assay based on partial sequences of the cytochrome oxidase I gene that can reliably identify the processed fins of seven of the eight CITES listed shark species. We also demonstrate that the assay can even frequently identify the species or genus of origin of shark fin soup (31 out of 50 samples). |
format |
Article in Journal/Newspaper |
author |
Andrew T Fields Debra L Abercrombie Rowena Eng Kevin Feldheim Demian D Chapman |
author_facet |
Andrew T Fields Debra L Abercrombie Rowena Eng Kevin Feldheim Demian D Chapman |
author_sort |
Andrew T Fields |
title |
A novel mini-DNA barcoding assay to identify processed fins from internationally protected shark species. |
title_short |
A novel mini-DNA barcoding assay to identify processed fins from internationally protected shark species. |
title_full |
A novel mini-DNA barcoding assay to identify processed fins from internationally protected shark species. |
title_fullStr |
A novel mini-DNA barcoding assay to identify processed fins from internationally protected shark species. |
title_full_unstemmed |
A novel mini-DNA barcoding assay to identify processed fins from internationally protected shark species. |
title_sort |
novel mini-dna barcoding assay to identify processed fins from internationally protected shark species. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0114844 https://doaj.org/article/e47c0fe3e3474ba99c5a9003ad2c5fb9 |
genre |
Cetorhinus maximus Lamna nasus Porbeagle |
genre_facet |
Cetorhinus maximus Lamna nasus Porbeagle |
op_source |
PLoS ONE, Vol 10, Iss 2, p e0114844 (2015) |
op_relation |
http://europepmc.org/articles/PMC4315593?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0114844 https://doaj.org/article/e47c0fe3e3474ba99c5a9003ad2c5fb9 |
op_doi |
https://doi.org/10.1371/journal.pone.0114844 |
container_title |
PLOS ONE |
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10 |
container_issue |
2 |
container_start_page |
e0114844 |
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1766389032511602688 |