Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants
Low temperature stress has a severe impact on the distribution, physiology, and survival of plants in their natural habitats. While numerous studies have focused on the physiological and molecular adjustments to low temperatures, this study provides evidence that cold induced physiological responses...
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ftdoajarticles:oai:doaj.org/article:e46222bb79ab49cea5f715831fbc7563 2023-05-15T18:04:31+02:00 Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants Philip Steiner Othmar Buchner Ancuela Andosch Gerhard Wanner Gilbert Neuner Ursula Lütz-Meindl 2020-11-01T00:00:00Z https://doi.org/10.3390/ijms21228753 https://doaj.org/article/e46222bb79ab49cea5f715831fbc7563 EN eng MDPI AG https://www.mdpi.com/1422-0067/21/22/8753 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms21228753 1422-0067 1661-6596 https://doaj.org/article/e46222bb79ab49cea5f715831fbc7563 International Journal of Molecular Sciences, Vol 21, Iss 8753, p 8753 (2020) electron microscopy TEM FIB-SEM Micrasterias denticulata Lemna sp Ranunculus glacialis Biology (General) QH301-705.5 Chemistry QD1-999 article 2020 ftdoajarticles https://doi.org/10.3390/ijms21228753 2022-12-31T12:09:18Z Low temperature stress has a severe impact on the distribution, physiology, and survival of plants in their natural habitats. While numerous studies have focused on the physiological and molecular adjustments to low temperatures, this study provides evidence that cold induced physiological responses coincide with distinct ultrastructural alterations. Three plants from different evolutionary levels and habitats were investigated: The freshwater alga Micrasterias denticulata , the aquatic plant Lemna sp. , and the nival plant Ranunculus glacialis. Ultrastructural alterations during low temperature stress were determined by the employment of 2-D transmission electron microscopy and 3-D reconstructions from focused ion beam–scanning electron microscopic series. With decreasing temperatures, increasing numbers of organelle contacts and particularly the fusion of mitochondria to 3-dimensional networks were observed. We assume that the increase or at least maintenance of respiration during low temperature stress is likely to be based on these mitochondrial interconnections. Moreover, it is shown that autophagy and degeneration processes accompany freezing stress in Lemna and R. glacialis . This might be an essential mechanism to recycle damaged cytoplasmic constituents to maintain the cellular metabolism during freezing stress. Article in Journal/Newspaper Ranunculus glacialis Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 21 22 8753 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
electron microscopy TEM FIB-SEM Micrasterias denticulata Lemna sp Ranunculus glacialis Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
electron microscopy TEM FIB-SEM Micrasterias denticulata Lemna sp Ranunculus glacialis Biology (General) QH301-705.5 Chemistry QD1-999 Philip Steiner Othmar Buchner Ancuela Andosch Gerhard Wanner Gilbert Neuner Ursula Lütz-Meindl Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants |
topic_facet |
electron microscopy TEM FIB-SEM Micrasterias denticulata Lemna sp Ranunculus glacialis Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Low temperature stress has a severe impact on the distribution, physiology, and survival of plants in their natural habitats. While numerous studies have focused on the physiological and molecular adjustments to low temperatures, this study provides evidence that cold induced physiological responses coincide with distinct ultrastructural alterations. Three plants from different evolutionary levels and habitats were investigated: The freshwater alga Micrasterias denticulata , the aquatic plant Lemna sp. , and the nival plant Ranunculus glacialis. Ultrastructural alterations during low temperature stress were determined by the employment of 2-D transmission electron microscopy and 3-D reconstructions from focused ion beam–scanning electron microscopic series. With decreasing temperatures, increasing numbers of organelle contacts and particularly the fusion of mitochondria to 3-dimensional networks were observed. We assume that the increase or at least maintenance of respiration during low temperature stress is likely to be based on these mitochondrial interconnections. Moreover, it is shown that autophagy and degeneration processes accompany freezing stress in Lemna and R. glacialis . This might be an essential mechanism to recycle damaged cytoplasmic constituents to maintain the cellular metabolism during freezing stress. |
format |
Article in Journal/Newspaper |
author |
Philip Steiner Othmar Buchner Ancuela Andosch Gerhard Wanner Gilbert Neuner Ursula Lütz-Meindl |
author_facet |
Philip Steiner Othmar Buchner Ancuela Andosch Gerhard Wanner Gilbert Neuner Ursula Lütz-Meindl |
author_sort |
Philip Steiner |
title |
Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants |
title_short |
Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants |
title_full |
Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants |
title_fullStr |
Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants |
title_full_unstemmed |
Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants |
title_sort |
fusion of mitochondria to 3-d networks, autophagy and increased organelle contacts are important subcellular hallmarks during cold stress in plants |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/ijms21228753 https://doaj.org/article/e46222bb79ab49cea5f715831fbc7563 |
genre |
Ranunculus glacialis |
genre_facet |
Ranunculus glacialis |
op_source |
International Journal of Molecular Sciences, Vol 21, Iss 8753, p 8753 (2020) |
op_relation |
https://www.mdpi.com/1422-0067/21/22/8753 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms21228753 1422-0067 1661-6596 https://doaj.org/article/e46222bb79ab49cea5f715831fbc7563 |
op_doi |
https://doi.org/10.3390/ijms21228753 |
container_title |
International Journal of Molecular Sciences |
container_volume |
21 |
container_issue |
22 |
container_start_page |
8753 |
_version_ |
1766175907770269696 |