Evaluation of a new fusion antigen, cd loop and HAP2-GCS1 domain (cd-HAP) of Plasmodium falciparum Generative Cell Specific 1 antigen formulated with various adjuvants, as a transmission blocking vaccine
Abstract Background Malaria is a major global health challenge, and for the elimination and eradication of this disease, transmission-blocking vaccines (TBVs) are a priority. Plasmodium falciparum Generative Cell Specific 1 (PfGCS1), a promising TBV candidate, is essential for gamete fertilization....
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ftdoajarticles:oai:doaj.org/article:e456798320eb40fdb50400042937b2e7 2024-01-14T10:05:08+01:00 Evaluation of a new fusion antigen, cd loop and HAP2-GCS1 domain (cd-HAP) of Plasmodium falciparum Generative Cell Specific 1 antigen formulated with various adjuvants, as a transmission blocking vaccine Zeinab Pourhashem Leila Nourani Jafar J. Sani Hemn Yousefi Sakineh Pirahmadi Mobina Sabouri Abbasali Raz Navid Dinparast Djadid Sedigheh Zakeri Akram Abouie Mehrizi 2023-12-01T00:00:00Z https://doi.org/10.1186/s12936-023-04798-7 https://doaj.org/article/e456798320eb40fdb50400042937b2e7 EN eng BMC https://doi.org/10.1186/s12936-023-04798-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-023-04798-7 1475-2875 https://doaj.org/article/e456798320eb40fdb50400042937b2e7 Malaria Journal, Vol 22, Iss 1, Pp 1-16 (2023) Malaria vaccine Cd-HAP antigen Adjuvants Immunogenicity SMFA Naturally acquired antibodies Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2023 ftdoajarticles https://doi.org/10.1186/s12936-023-04798-7 2023-12-17T01:48:06Z Abstract Background Malaria is a major global health challenge, and for the elimination and eradication of this disease, transmission-blocking vaccines (TBVs) are a priority. Plasmodium falciparum Generative Cell Specific 1 (PfGCS1), a promising TBV candidate, is essential for gamete fertilization. The HAP2-GCS1 domain of this antigen as well as its cd loop could induce antibodies that partially inhibit transmission of P. falciparum. Methods In the current study, a new synthetic fusion antigen containing cd loop and HAP2-GCS1 domain (cd-HAP) of PfGCS1 was evaluated as a transmission blocking vaccine candidate. Initially, the profile of naturally acquired IgG antibodies to the cd-HAP antigen was analysed in Iranian individuals infected with P. falciparum, to confirm that this new fusion protein has the appropriate structure containing common epitopes with the native form of PfGCS1. Then, the immunogenicity of cd-HAP was evaluated in BALB/c mice, using different adjuvant systems such as CpG, MPL, QS-21, and a combination of them (CMQ). Furthermore, the blocking efficacy of polyclonal antibodies induced against these formulations was also assessed by oocyst intensity and infection prevalence in the Standard Membrane Feeding Assay (SMFA). Results The naturally acquired antibodies (dominantly IgG1 and IgG3 subclasses) induced in P. falciparum-infected individuals could recognize the cd-HAP antigen which implies that the new fusion protein has a proper conformation that mimics the native structure of PfGCS1. Concerning the immunogenicity of cd-HAP antigen, the highest IgG levels and titers, by a Th1-type immune profile, and elevated antibody avidity were induced in mice immunized with the cd-HAP antigen formulated with a combination of adjuvants (P < 0.0001). Additionally, cytokine profiling of the immunized mice displayed that a high level of IFN-γ response, a Th1-type immune response, was produced by splenocytes from immunized mice that received cd-HAP antigen in combination with CMQ adjuvants (P < 0.0001). ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 22 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Malaria vaccine Cd-HAP antigen Adjuvants Immunogenicity SMFA Naturally acquired antibodies Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria vaccine Cd-HAP antigen Adjuvants Immunogenicity SMFA Naturally acquired antibodies Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Zeinab Pourhashem Leila Nourani Jafar J. Sani Hemn Yousefi Sakineh Pirahmadi Mobina Sabouri Abbasali Raz Navid Dinparast Djadid Sedigheh Zakeri Akram Abouie Mehrizi Evaluation of a new fusion antigen, cd loop and HAP2-GCS1 domain (cd-HAP) of Plasmodium falciparum Generative Cell Specific 1 antigen formulated with various adjuvants, as a transmission blocking vaccine |
topic_facet |
Malaria vaccine Cd-HAP antigen Adjuvants Immunogenicity SMFA Naturally acquired antibodies Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria is a major global health challenge, and for the elimination and eradication of this disease, transmission-blocking vaccines (TBVs) are a priority. Plasmodium falciparum Generative Cell Specific 1 (PfGCS1), a promising TBV candidate, is essential for gamete fertilization. The HAP2-GCS1 domain of this antigen as well as its cd loop could induce antibodies that partially inhibit transmission of P. falciparum. Methods In the current study, a new synthetic fusion antigen containing cd loop and HAP2-GCS1 domain (cd-HAP) of PfGCS1 was evaluated as a transmission blocking vaccine candidate. Initially, the profile of naturally acquired IgG antibodies to the cd-HAP antigen was analysed in Iranian individuals infected with P. falciparum, to confirm that this new fusion protein has the appropriate structure containing common epitopes with the native form of PfGCS1. Then, the immunogenicity of cd-HAP was evaluated in BALB/c mice, using different adjuvant systems such as CpG, MPL, QS-21, and a combination of them (CMQ). Furthermore, the blocking efficacy of polyclonal antibodies induced against these formulations was also assessed by oocyst intensity and infection prevalence in the Standard Membrane Feeding Assay (SMFA). Results The naturally acquired antibodies (dominantly IgG1 and IgG3 subclasses) induced in P. falciparum-infected individuals could recognize the cd-HAP antigen which implies that the new fusion protein has a proper conformation that mimics the native structure of PfGCS1. Concerning the immunogenicity of cd-HAP antigen, the highest IgG levels and titers, by a Th1-type immune profile, and elevated antibody avidity were induced in mice immunized with the cd-HAP antigen formulated with a combination of adjuvants (P < 0.0001). Additionally, cytokine profiling of the immunized mice displayed that a high level of IFN-γ response, a Th1-type immune response, was produced by splenocytes from immunized mice that received cd-HAP antigen in combination with CMQ adjuvants (P < 0.0001). ... |
format |
Article in Journal/Newspaper |
author |
Zeinab Pourhashem Leila Nourani Jafar J. Sani Hemn Yousefi Sakineh Pirahmadi Mobina Sabouri Abbasali Raz Navid Dinparast Djadid Sedigheh Zakeri Akram Abouie Mehrizi |
author_facet |
Zeinab Pourhashem Leila Nourani Jafar J. Sani Hemn Yousefi Sakineh Pirahmadi Mobina Sabouri Abbasali Raz Navid Dinparast Djadid Sedigheh Zakeri Akram Abouie Mehrizi |
author_sort |
Zeinab Pourhashem |
title |
Evaluation of a new fusion antigen, cd loop and HAP2-GCS1 domain (cd-HAP) of Plasmodium falciparum Generative Cell Specific 1 antigen formulated with various adjuvants, as a transmission blocking vaccine |
title_short |
Evaluation of a new fusion antigen, cd loop and HAP2-GCS1 domain (cd-HAP) of Plasmodium falciparum Generative Cell Specific 1 antigen formulated with various adjuvants, as a transmission blocking vaccine |
title_full |
Evaluation of a new fusion antigen, cd loop and HAP2-GCS1 domain (cd-HAP) of Plasmodium falciparum Generative Cell Specific 1 antigen formulated with various adjuvants, as a transmission blocking vaccine |
title_fullStr |
Evaluation of a new fusion antigen, cd loop and HAP2-GCS1 domain (cd-HAP) of Plasmodium falciparum Generative Cell Specific 1 antigen formulated with various adjuvants, as a transmission blocking vaccine |
title_full_unstemmed |
Evaluation of a new fusion antigen, cd loop and HAP2-GCS1 domain (cd-HAP) of Plasmodium falciparum Generative Cell Specific 1 antigen formulated with various adjuvants, as a transmission blocking vaccine |
title_sort |
evaluation of a new fusion antigen, cd loop and hap2-gcs1 domain (cd-hap) of plasmodium falciparum generative cell specific 1 antigen formulated with various adjuvants, as a transmission blocking vaccine |
publisher |
BMC |
publishDate |
2023 |
url |
https://doi.org/10.1186/s12936-023-04798-7 https://doaj.org/article/e456798320eb40fdb50400042937b2e7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 22, Iss 1, Pp 1-16 (2023) |
op_relation |
https://doi.org/10.1186/s12936-023-04798-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-023-04798-7 1475-2875 https://doaj.org/article/e456798320eb40fdb50400042937b2e7 |
op_doi |
https://doi.org/10.1186/s12936-023-04798-7 |
container_title |
Malaria Journal |
container_volume |
22 |
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1 |
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1788059532494110720 |