Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats
Mutations in Exon 1, 2 and 3 of the vitamin K epoxide reductase complex subunit 1 (Vkorc1) gene are known to lead to anticoagulant rodenticide resistance. In order to investigate their putative resistance in rodenticides, we studied the genetic profile of the Vkorc1 gene in Turkish black rats (Rattu...
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ftdoajarticles:oai:doaj.org/article:e41823959f0c42fbb04c2a906688dc4b 2024-01-07T09:46:13+01:00 Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats Nuri Yiğit Mustafa T. Duman Derya Çetintürk Fulya Saygılı-Yiğit Ercüment Çolak Reyhan Çolak 2023-05-01T00:00:00Z https://doi.org/10.7717/peerj.15055 https://doaj.org/article/e41823959f0c42fbb04c2a906688dc4b EN eng PeerJ Inc. https://peerj.com/articles/15055.pdf https://peerj.com/articles/15055/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.15055 2167-8359 https://doaj.org/article/e41823959f0c42fbb04c2a906688dc4b PeerJ, Vol 11, p e15055 (2023) Vkorc1 Anticoagulant resistance Mutation Black rat Brown rat Medicine R Biology (General) QH301-705.5 article 2023 ftdoajarticles https://doi.org/10.7717/peerj.15055 2023-12-10T01:49:51Z Mutations in Exon 1, 2 and 3 of the vitamin K epoxide reductase complex subunit 1 (Vkorc1) gene are known to lead to anticoagulant rodenticide resistance. In order to investigate their putative resistance in rodenticides, we studied the genetic profile of the Vkorc1 gene in Turkish black rats (Rattus rattus) and brown rats (Rattus norvegicus). In this context, previously recorded Ala21Thr mutation (R. rattus) in Exon 1 region, Ile90Leu mutation (R. rattus, R. norvegicus) in Exon 2 region and Leu120Gln mutation (R. norvegicus) in Exon 3 region were identified as “missense mutations” causing amino acid changes. Ala21Thr mutation was first detected in one specimen of Turkish black rat despite the uncertainty of its relevance to resistance. Ile90Leu mutation accepted as neutral variant was detected in most of black rat specimens. Leu120Gln mutation related to anticoagulant rodenticide resistance was found in only one brown rat specimen. Furthermore, Ser74Asn, Gln77Pro (black rat) and Ser79Pro (brown rat) mutations that cause amino acid changes in the Exon 2 region but unclear whether they cause resistance were identified. In addition, “silent mutations” which do not cause amino acid changes were also defined; these mutations were Arg12Arg mutation in Exon 1 region, His68His, Ser81Ser, Ile82Ile and Leu94Leu mutations in Exon 2 region and Ile107Ile, Thr137Thr, Ala143Ala and Gln152Gln mutations in Exon 3 region. These silent mutations were found in both species except for Ser81Ser which was determined in only brown rats. Article in Journal/Newspaper Rattus rattus Directory of Open Access Journals: DOAJ Articles PeerJ 11 e15055 |
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Vkorc1 Anticoagulant resistance Mutation Black rat Brown rat Medicine R Biology (General) QH301-705.5 |
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Vkorc1 Anticoagulant resistance Mutation Black rat Brown rat Medicine R Biology (General) QH301-705.5 Nuri Yiğit Mustafa T. Duman Derya Çetintürk Fulya Saygılı-Yiğit Ercüment Çolak Reyhan Çolak Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats |
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Vkorc1 Anticoagulant resistance Mutation Black rat Brown rat Medicine R Biology (General) QH301-705.5 |
description |
Mutations in Exon 1, 2 and 3 of the vitamin K epoxide reductase complex subunit 1 (Vkorc1) gene are known to lead to anticoagulant rodenticide resistance. In order to investigate their putative resistance in rodenticides, we studied the genetic profile of the Vkorc1 gene in Turkish black rats (Rattus rattus) and brown rats (Rattus norvegicus). In this context, previously recorded Ala21Thr mutation (R. rattus) in Exon 1 region, Ile90Leu mutation (R. rattus, R. norvegicus) in Exon 2 region and Leu120Gln mutation (R. norvegicus) in Exon 3 region were identified as “missense mutations” causing amino acid changes. Ala21Thr mutation was first detected in one specimen of Turkish black rat despite the uncertainty of its relevance to resistance. Ile90Leu mutation accepted as neutral variant was detected in most of black rat specimens. Leu120Gln mutation related to anticoagulant rodenticide resistance was found in only one brown rat specimen. Furthermore, Ser74Asn, Gln77Pro (black rat) and Ser79Pro (brown rat) mutations that cause amino acid changes in the Exon 2 region but unclear whether they cause resistance were identified. In addition, “silent mutations” which do not cause amino acid changes were also defined; these mutations were Arg12Arg mutation in Exon 1 region, His68His, Ser81Ser, Ile82Ile and Leu94Leu mutations in Exon 2 region and Ile107Ile, Thr137Thr, Ala143Ala and Gln152Gln mutations in Exon 3 region. These silent mutations were found in both species except for Ser81Ser which was determined in only brown rats. |
format |
Article in Journal/Newspaper |
author |
Nuri Yiğit Mustafa T. Duman Derya Çetintürk Fulya Saygılı-Yiğit Ercüment Çolak Reyhan Çolak |
author_facet |
Nuri Yiğit Mustafa T. Duman Derya Çetintürk Fulya Saygılı-Yiğit Ercüment Çolak Reyhan Çolak |
author_sort |
Nuri Yiğit |
title |
Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats |
title_short |
Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats |
title_full |
Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats |
title_fullStr |
Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats |
title_full_unstemmed |
Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats |
title_sort |
vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in turkish rats |
publisher |
PeerJ Inc. |
publishDate |
2023 |
url |
https://doi.org/10.7717/peerj.15055 https://doaj.org/article/e41823959f0c42fbb04c2a906688dc4b |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
PeerJ, Vol 11, p e15055 (2023) |
op_relation |
https://peerj.com/articles/15055.pdf https://peerj.com/articles/15055/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.15055 2167-8359 https://doaj.org/article/e41823959f0c42fbb04c2a906688dc4b |
op_doi |
https://doi.org/10.7717/peerj.15055 |
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PeerJ |
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11 |
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e15055 |
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1787427947366318080 |