An automated haematology analyzer XN-30 distinguishes developmental stages of falciparum malaria parasite cultured in vitro
Abstract Background The automated haematology analyzer XN-30 (Sysmex, Kobe, Japan) easily and rapidly detects malarial parasites in clinical blood samples using flow cytometry. The XN-30 analyzer is able to distinguish each developmental stage by measuring DNA content and cell size. Thus, it was exp...
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ftdoajarticles:oai:doaj.org/article:e3c8a1e81fb04629b5da8b1a8c61cd77 2023-05-15T15:08:04+02:00 An automated haematology analyzer XN-30 distinguishes developmental stages of falciparum malaria parasite cultured in vitro Takahiro Tougan Yuhgi Suzuki Sawako Itagaki Munehisa Izuka Yuji Toya Kinya Uchihashi Toshihiro Horii 2018-02-01T00:00:00Z https://doi.org/10.1186/s12936-018-2208-6 https://doaj.org/article/e3c8a1e81fb04629b5da8b1a8c61cd77 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2208-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2208-6 1475-2875 https://doaj.org/article/e3c8a1e81fb04629b5da8b1a8c61cd77 Malaria Journal, Vol 17, Iss 1, Pp 1-8 (2018) Plasmodium falciparum in vitro culture Blood stage Flow cytometry XN-30 analyzer Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2208-6 2022-12-31T03:49:10Z Abstract Background The automated haematology analyzer XN-30 (Sysmex, Kobe, Japan) easily and rapidly detects malarial parasites in clinical blood samples using flow cytometry. The XN-30 analyzer is able to distinguish each developmental stage by measuring DNA content and cell size. Thus, it was expected to be capable of quantifying the developmental stages of cultured falciparum parasite. To achieve this requirement, a modified algorithm was tested for its validity and reliability using in vitro cultured falciparum parasite. Results The XN-30 analyzer automatically measured each developmental stage as well as total parasitaemia. Comparison of the parasitaemia obtained using the XN-30 analyzer equipped with the modified algorithm with that obtained using microscopy examination of Giemsa-stained smears revealed the greater sensitivity and reproducibility of the former. The XN-30 analyzer also detected free merozoites and purified gametocytes. Conclusions The XN-30 analyzer allows the precise recognition and enumeration of total and each developmental stages of cultured falciparum parasites, and permits the sensitive and reproducible calculation of parasitaemia. The results indicate the potential of the XN-30 analyzer for basic research on malarial biology, anti-malarial drug discovery, and evaluation of drug efficacy. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 17 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Plasmodium falciparum in vitro culture Blood stage Flow cytometry XN-30 analyzer Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium falciparum in vitro culture Blood stage Flow cytometry XN-30 analyzer Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Takahiro Tougan Yuhgi Suzuki Sawako Itagaki Munehisa Izuka Yuji Toya Kinya Uchihashi Toshihiro Horii An automated haematology analyzer XN-30 distinguishes developmental stages of falciparum malaria parasite cultured in vitro |
topic_facet |
Plasmodium falciparum in vitro culture Blood stage Flow cytometry XN-30 analyzer Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The automated haematology analyzer XN-30 (Sysmex, Kobe, Japan) easily and rapidly detects malarial parasites in clinical blood samples using flow cytometry. The XN-30 analyzer is able to distinguish each developmental stage by measuring DNA content and cell size. Thus, it was expected to be capable of quantifying the developmental stages of cultured falciparum parasite. To achieve this requirement, a modified algorithm was tested for its validity and reliability using in vitro cultured falciparum parasite. Results The XN-30 analyzer automatically measured each developmental stage as well as total parasitaemia. Comparison of the parasitaemia obtained using the XN-30 analyzer equipped with the modified algorithm with that obtained using microscopy examination of Giemsa-stained smears revealed the greater sensitivity and reproducibility of the former. The XN-30 analyzer also detected free merozoites and purified gametocytes. Conclusions The XN-30 analyzer allows the precise recognition and enumeration of total and each developmental stages of cultured falciparum parasites, and permits the sensitive and reproducible calculation of parasitaemia. The results indicate the potential of the XN-30 analyzer for basic research on malarial biology, anti-malarial drug discovery, and evaluation of drug efficacy. |
format |
Article in Journal/Newspaper |
author |
Takahiro Tougan Yuhgi Suzuki Sawako Itagaki Munehisa Izuka Yuji Toya Kinya Uchihashi Toshihiro Horii |
author_facet |
Takahiro Tougan Yuhgi Suzuki Sawako Itagaki Munehisa Izuka Yuji Toya Kinya Uchihashi Toshihiro Horii |
author_sort |
Takahiro Tougan |
title |
An automated haematology analyzer XN-30 distinguishes developmental stages of falciparum malaria parasite cultured in vitro |
title_short |
An automated haematology analyzer XN-30 distinguishes developmental stages of falciparum malaria parasite cultured in vitro |
title_full |
An automated haematology analyzer XN-30 distinguishes developmental stages of falciparum malaria parasite cultured in vitro |
title_fullStr |
An automated haematology analyzer XN-30 distinguishes developmental stages of falciparum malaria parasite cultured in vitro |
title_full_unstemmed |
An automated haematology analyzer XN-30 distinguishes developmental stages of falciparum malaria parasite cultured in vitro |
title_sort |
automated haematology analyzer xn-30 distinguishes developmental stages of falciparum malaria parasite cultured in vitro |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2208-6 https://doaj.org/article/e3c8a1e81fb04629b5da8b1a8c61cd77 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-8 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2208-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2208-6 1475-2875 https://doaj.org/article/e3c8a1e81fb04629b5da8b1a8c61cd77 |
op_doi |
https://doi.org/10.1186/s12936-018-2208-6 |
container_title |
Malaria Journal |
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17 |
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1 |
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1766339495141048320 |