A morphological protocol and guide-list on uterine cervix cytology associated to Papillomavirus infection Padronização da análise de critérios citomorfológicos de lesões associadas à infecção pelo HPV
The present study was designed to further assess the validity of the cytological description of morphological lesions said to be related to Papillomavirus (HPV) infections in senior women. The casuistic comprised 196 cervical smears from a group of women with no clinical or morphological evidence of...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Language: | English |
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Universidade de São Paulo (USP)
2004
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Online Access: | https://doi.org/10.1590/S0036-46652004000400003 https://doaj.org/article/e3bdb762349c4929be41aeab36078b2d |
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ftdoajarticles:oai:doaj.org/article:e3bdb762349c4929be41aeab36078b2d 2024-09-09T19:28:09+00:00 A morphological protocol and guide-list on uterine cervix cytology associated to Papillomavirus infection Padronização da análise de critérios citomorfológicos de lesões associadas à infecção pelo HPV Luzia Setuko Umeda Yamamoto Venâncio Avancini Ferreira Alves Marina Yoshiê Sakamoto Maeda Adhemar Longatto-Filho Maria Lucia Utagawa José Eluf Neto 2004-08-01T00:00:00Z https://doi.org/10.1590/S0036-46652004000400003 https://doaj.org/article/e3bdb762349c4929be41aeab36078b2d EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652004000400003 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652004000400003 0036-4665 1678-9946 https://doaj.org/article/e3bdb762349c4929be41aeab36078b2d Revista do Instituto de Medicina Tropical de São Paulo, Vol 46, Iss 4, Pp 189-193 (2004) HPV infection PCR Cervical smears Cytomorphology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2004 ftdoajarticles https://doi.org/10.1590/S0036-46652004000400003 2024-08-05T17:49:31Z The present study was designed to further assess the validity of the cytological description of morphological lesions said to be related to Papillomavirus (HPV) infections in senior women. The casuistic comprised 196 cervical smears from a group of women with no clinical or morphological evidence of neoplasia, collected simultaneously with samples submitted to detection of HPV DNA by PCR in a previous study. Three experienced cytologists studied each slide in two different conditions, with an interval of 20 months between them. The first approach was performed under routine laboratory standards, whereas the second was guided by a list of 16 well-defined parameters indicative of HPV-related cytological lesions. When suspicious cases of HPV-related alterations were grouped with positive cases, they showed on average: sensitivity of 25.5%, specificity of 84.4% and positive predictive value (PPV) of 26.8%. When suspicious cases were grouped with negative cases, sensitivity decreased, whereas specificity and PPV increased, as expected. In the second reading, which followed a "guide-list", a decrease in sensitivity was observed, contrasting with a sharp increase of positive predictive value. Among the 16 cytomorphological criteria tested, "koilocytosis", "mild koilocytosis" and "condylomatous parabasal cells" yielded the best predictive value for HPV DNA detection by PCR. In conclusion, despite the low sensitivity, cytopathologic assessment of cervico-vaginal smears leads to a highly specific diagnosis of HPV infection in menopausal women, with PPV of 91.0% when directed by a guide-list of well-defined morphologic criteria. O presente estudo analisou aspectos relacionados ao valor da citologia cervical na identificação de alterações cito-morfológicas relacionadas à infecção por Papilomavírus humano (HPV) em mulheres com idades avançadas. A casuística compreendeu 196 amostras cérvico-vaginais provenientes de uma população de mulheres sem evidências clínicas ou citológicas de neoplasia cervical. As amostras foram ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 46 4 189 193 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
HPV infection PCR Cervical smears Cytomorphology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
HPV infection PCR Cervical smears Cytomorphology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Luzia Setuko Umeda Yamamoto Venâncio Avancini Ferreira Alves Marina Yoshiê Sakamoto Maeda Adhemar Longatto-Filho Maria Lucia Utagawa José Eluf Neto A morphological protocol and guide-list on uterine cervix cytology associated to Papillomavirus infection Padronização da análise de critérios citomorfológicos de lesões associadas à infecção pelo HPV |
topic_facet |
HPV infection PCR Cervical smears Cytomorphology Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
The present study was designed to further assess the validity of the cytological description of morphological lesions said to be related to Papillomavirus (HPV) infections in senior women. The casuistic comprised 196 cervical smears from a group of women with no clinical or morphological evidence of neoplasia, collected simultaneously with samples submitted to detection of HPV DNA by PCR in a previous study. Three experienced cytologists studied each slide in two different conditions, with an interval of 20 months between them. The first approach was performed under routine laboratory standards, whereas the second was guided by a list of 16 well-defined parameters indicative of HPV-related cytological lesions. When suspicious cases of HPV-related alterations were grouped with positive cases, they showed on average: sensitivity of 25.5%, specificity of 84.4% and positive predictive value (PPV) of 26.8%. When suspicious cases were grouped with negative cases, sensitivity decreased, whereas specificity and PPV increased, as expected. In the second reading, which followed a "guide-list", a decrease in sensitivity was observed, contrasting with a sharp increase of positive predictive value. Among the 16 cytomorphological criteria tested, "koilocytosis", "mild koilocytosis" and "condylomatous parabasal cells" yielded the best predictive value for HPV DNA detection by PCR. In conclusion, despite the low sensitivity, cytopathologic assessment of cervico-vaginal smears leads to a highly specific diagnosis of HPV infection in menopausal women, with PPV of 91.0% when directed by a guide-list of well-defined morphologic criteria. O presente estudo analisou aspectos relacionados ao valor da citologia cervical na identificação de alterações cito-morfológicas relacionadas à infecção por Papilomavírus humano (HPV) em mulheres com idades avançadas. A casuística compreendeu 196 amostras cérvico-vaginais provenientes de uma população de mulheres sem evidências clínicas ou citológicas de neoplasia cervical. As amostras foram ... |
format |
Article in Journal/Newspaper |
author |
Luzia Setuko Umeda Yamamoto Venâncio Avancini Ferreira Alves Marina Yoshiê Sakamoto Maeda Adhemar Longatto-Filho Maria Lucia Utagawa José Eluf Neto |
author_facet |
Luzia Setuko Umeda Yamamoto Venâncio Avancini Ferreira Alves Marina Yoshiê Sakamoto Maeda Adhemar Longatto-Filho Maria Lucia Utagawa José Eluf Neto |
author_sort |
Luzia Setuko Umeda Yamamoto |
title |
A morphological protocol and guide-list on uterine cervix cytology associated to Papillomavirus infection Padronização da análise de critérios citomorfológicos de lesões associadas à infecção pelo HPV |
title_short |
A morphological protocol and guide-list on uterine cervix cytology associated to Papillomavirus infection Padronização da análise de critérios citomorfológicos de lesões associadas à infecção pelo HPV |
title_full |
A morphological protocol and guide-list on uterine cervix cytology associated to Papillomavirus infection Padronização da análise de critérios citomorfológicos de lesões associadas à infecção pelo HPV |
title_fullStr |
A morphological protocol and guide-list on uterine cervix cytology associated to Papillomavirus infection Padronização da análise de critérios citomorfológicos de lesões associadas à infecção pelo HPV |
title_full_unstemmed |
A morphological protocol and guide-list on uterine cervix cytology associated to Papillomavirus infection Padronização da análise de critérios citomorfológicos de lesões associadas à infecção pelo HPV |
title_sort |
morphological protocol and guide-list on uterine cervix cytology associated to papillomavirus infection padronização da análise de critérios citomorfológicos de lesões associadas à infecção pelo hpv |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2004 |
url |
https://doi.org/10.1590/S0036-46652004000400003 https://doaj.org/article/e3bdb762349c4929be41aeab36078b2d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 46, Iss 4, Pp 189-193 (2004) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652004000400003 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652004000400003 0036-4665 1678-9946 https://doaj.org/article/e3bdb762349c4929be41aeab36078b2d |
op_doi |
https://doi.org/10.1590/S0036-46652004000400003 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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46 |
container_issue |
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189 |
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