Producción de biofilm y presencia de genes icaABCD en cepas de Staphylococcus aureus aisladas de leche cruda
Abstract: In the pathogenesis of staphylococcal mastitis the biofilm is considered a marker of virulence that allows the adhesion of the microorganism to the mammary epithelium. In order to determine the production of biofilm and the presence of icaABCD gene, 30 S. aureus strains isolated from raw m...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2017
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ftdoajarticles:oai:doaj.org/article:e39ffbd96ade4a6999f5443fde291786 2023-10-09T21:49:33+02:00 Producción de biofilm y presencia de genes icaABCD en cepas de Staphylococcus aureus aisladas de leche cruda Ginestre P Messaria Ávila R Yeiny Valero L Kutchynskaya Rivera S Jhoandry Briñez Z Wilfrido Valeris C Robert 2017-12-01T00:00:00Z https://doaj.org/article/e39ffbd96ade4a6999f5443fde291786 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://sites.google.com/view/revistakasmera/vol%C3%BAmenes/2016-2020-vols-44-47/2017-v-45-n-2/79-87?authuser=0 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/e39ffbd96ade4a6999f5443fde291786 Kasmera, Vol 45, Iss 2, Pp 79-87 (2017) Biofilm S. aureus genes ica leche cruda ica genes raw milk Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles 2023-09-10T00:51:04Z Abstract: In the pathogenesis of staphylococcal mastitis the biofilm is considered a marker of virulence that allows the adhesion of the microorganism to the mammary epithelium. In order to determine the production of biofilm and the presence of icaABCD gene, 30 S. aureus strains isolated from raw milk obtained from cows with subclinical mastitis were analyzed. Biofilm production was determined by the quantitative method on microplates for cell culture and the presence of genes icaABCD by polymerase chain reaction. Biofilm production was observed in 96.67% of strains of S. aureus. These 43.34% were strong producers, 30.00% producing moderate, 23.33% weak producers and 3.33% non producing biofilm. All of the icaABCD genes was obtained at 6.66% of the isolates. All strains, except one, presented icaA or icaD. 56,66% of isolates identified genes icaA/icaD. The icaABCD operon genes were identified in all biofilm-producing strains, which demonstrates that they possess a virulence factor that facilitates its persistence in the breast tissue. In addition, the biofilm is a source of microbial contamination which can lead to failures in production processes that generate problems of food safety. Resumen: En la patogénesis de la mastitis estafilocócica, el biofilm se considera un marcador de virulencia que permite la adherencia del microorganismo al epitelio mamario. Con la finalidad de determinar la producción de biofilm y detectar la presencia de genes icaABCD, se analizaron 30 cepas de S. aureus aisladas de leche cruda obtenida de vacas con mastitis subclínica. El biofilm se determinó por el método cuantitativo sobre microplacas de cultivo celular y los genes icaABCD por reacción en cadena de la polimerasa. En 96,67% de las cepas de S. aureus se observó producción de biofilm. De éstas 43,34% fueron fuertes productoras, 30,00% moderadas productoras, 23,33% débiles productoras y 3,33% no productoras de biofilm. La totalidad de los genes icaADCD se obtuvo en 6,66% de los aislamientos. Todas las cepas, excepto una, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Cadena ENVELOPE(-67.600,-67.600,-67.450,-67.450) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
Biofilm S. aureus genes ica leche cruda ica genes raw milk Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Biofilm S. aureus genes ica leche cruda ica genes raw milk Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ginestre P Messaria Ávila R Yeiny Valero L Kutchynskaya Rivera S Jhoandry Briñez Z Wilfrido Valeris C Robert Producción de biofilm y presencia de genes icaABCD en cepas de Staphylococcus aureus aisladas de leche cruda |
topic_facet |
Biofilm S. aureus genes ica leche cruda ica genes raw milk Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Abstract: In the pathogenesis of staphylococcal mastitis the biofilm is considered a marker of virulence that allows the adhesion of the microorganism to the mammary epithelium. In order to determine the production of biofilm and the presence of icaABCD gene, 30 S. aureus strains isolated from raw milk obtained from cows with subclinical mastitis were analyzed. Biofilm production was determined by the quantitative method on microplates for cell culture and the presence of genes icaABCD by polymerase chain reaction. Biofilm production was observed in 96.67% of strains of S. aureus. These 43.34% were strong producers, 30.00% producing moderate, 23.33% weak producers and 3.33% non producing biofilm. All of the icaABCD genes was obtained at 6.66% of the isolates. All strains, except one, presented icaA or icaD. 56,66% of isolates identified genes icaA/icaD. The icaABCD operon genes were identified in all biofilm-producing strains, which demonstrates that they possess a virulence factor that facilitates its persistence in the breast tissue. In addition, the biofilm is a source of microbial contamination which can lead to failures in production processes that generate problems of food safety. Resumen: En la patogénesis de la mastitis estafilocócica, el biofilm se considera un marcador de virulencia que permite la adherencia del microorganismo al epitelio mamario. Con la finalidad de determinar la producción de biofilm y detectar la presencia de genes icaABCD, se analizaron 30 cepas de S. aureus aisladas de leche cruda obtenida de vacas con mastitis subclínica. El biofilm se determinó por el método cuantitativo sobre microplacas de cultivo celular y los genes icaABCD por reacción en cadena de la polimerasa. En 96,67% de las cepas de S. aureus se observó producción de biofilm. De éstas 43,34% fueron fuertes productoras, 30,00% moderadas productoras, 23,33% débiles productoras y 3,33% no productoras de biofilm. La totalidad de los genes icaADCD se obtuvo en 6,66% de los aislamientos. Todas las cepas, excepto una, ... |
format |
Article in Journal/Newspaper |
author |
Ginestre P Messaria Ávila R Yeiny Valero L Kutchynskaya Rivera S Jhoandry Briñez Z Wilfrido Valeris C Robert |
author_facet |
Ginestre P Messaria Ávila R Yeiny Valero L Kutchynskaya Rivera S Jhoandry Briñez Z Wilfrido Valeris C Robert |
author_sort |
Ginestre P Messaria |
title |
Producción de biofilm y presencia de genes icaABCD en cepas de Staphylococcus aureus aisladas de leche cruda |
title_short |
Producción de biofilm y presencia de genes icaABCD en cepas de Staphylococcus aureus aisladas de leche cruda |
title_full |
Producción de biofilm y presencia de genes icaABCD en cepas de Staphylococcus aureus aisladas de leche cruda |
title_fullStr |
Producción de biofilm y presencia de genes icaABCD en cepas de Staphylococcus aureus aisladas de leche cruda |
title_full_unstemmed |
Producción de biofilm y presencia de genes icaABCD en cepas de Staphylococcus aureus aisladas de leche cruda |
title_sort |
producción de biofilm y presencia de genes icaabcd en cepas de staphylococcus aureus aisladas de leche cruda |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2017 |
url |
https://doaj.org/article/e39ffbd96ade4a6999f5443fde291786 |
long_lat |
ENVELOPE(-67.600,-67.600,-67.450,-67.450) |
geographic |
Arctic Cadena |
geographic_facet |
Arctic Cadena |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 45, Iss 2, Pp 79-87 (2017) |
op_relation |
https://sites.google.com/view/revistakasmera/vol%C3%BAmenes/2016-2020-vols-44-47/2017-v-45-n-2/79-87?authuser=0 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/e39ffbd96ade4a6999f5443fde291786 |
_version_ |
1779312577002602496 |