Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae
Abstract Background Anopheles sinensis is a dominant natural vector of Plasmodium vivax in China, Taiwan, Japan, and Korea. Recent genome sequencing of An. sinensis provides important insights into the genomic basis of vectorial capacity. However, the lack of a physical genome map with chromosome as...
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ftdoajarticles:oai:doaj.org/article:e39d2f736fec439f97391627ec18db62 2023-05-15T15:06:42+02:00 Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae Yun Wei Biao Cheng Guoding Zhu Danyu Shen Jiangtao Liang Cong Wang Jing Wang Jianxia Tang Jun Cao Igor V. Sharakhov Ai Xia 2017-06-01T00:00:00Z https://doi.org/10.1186/s12936-017-1888-7 https://doaj.org/article/e39d2f736fec439f97391627ec18db62 EN eng BMC http://link.springer.com/article/10.1186/s12936-017-1888-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1888-7 1475-2875 https://doaj.org/article/e39d2f736fec439f97391627ec18db62 Malaria Journal, Vol 16, Iss 1, Pp 1-10 (2017) Chromosomal evolution GRIMM Inversion fixation Fluorescence in situ hybridization OrthoDB Synteny blocks Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-1888-7 2022-12-31T02:01:00Z Abstract Background Anopheles sinensis is a dominant natural vector of Plasmodium vivax in China, Taiwan, Japan, and Korea. Recent genome sequencing of An. sinensis provides important insights into the genomic basis of vectorial capacity. However, the lack of a physical genome map with chromosome assignment and orientation of sequencing scaffolds hinders comparative analyses with other genomes to infer evolutionary changes relevant to the vector capacity. Results Here, a physical genome map for An. sinensis was constructed by assigning 52 scaffolds onto the chromosomes using fluorescence in situ hybridization (FISH). This chromosome-based genome assembly composes approximately 36% of the total An. sinensis genome. Comparisons of 3955 orthologous genes between An. sinensis and Anopheles gambiae identified 361 conserved synteny blocks and 267 inversions fixed between these two lineages. The rate of gene order reshuffling on the X chromosome is approximately 3.2 times higher than that on the autosomes. Conclusions The physical map will facilitate detailed genomic analysis of An. sinensis and contribute to understanding of the patterns and mechanisms of large-scale genome rearrangements in anopheline mosquitoes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Chromosomal evolution GRIMM Inversion fixation Fluorescence in situ hybridization OrthoDB Synteny blocks Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Chromosomal evolution GRIMM Inversion fixation Fluorescence in situ hybridization OrthoDB Synteny blocks Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Yun Wei Biao Cheng Guoding Zhu Danyu Shen Jiangtao Liang Cong Wang Jing Wang Jianxia Tang Jun Cao Igor V. Sharakhov Ai Xia Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae |
topic_facet |
Chromosomal evolution GRIMM Inversion fixation Fluorescence in situ hybridization OrthoDB Synteny blocks Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Anopheles sinensis is a dominant natural vector of Plasmodium vivax in China, Taiwan, Japan, and Korea. Recent genome sequencing of An. sinensis provides important insights into the genomic basis of vectorial capacity. However, the lack of a physical genome map with chromosome assignment and orientation of sequencing scaffolds hinders comparative analyses with other genomes to infer evolutionary changes relevant to the vector capacity. Results Here, a physical genome map for An. sinensis was constructed by assigning 52 scaffolds onto the chromosomes using fluorescence in situ hybridization (FISH). This chromosome-based genome assembly composes approximately 36% of the total An. sinensis genome. Comparisons of 3955 orthologous genes between An. sinensis and Anopheles gambiae identified 361 conserved synteny blocks and 267 inversions fixed between these two lineages. The rate of gene order reshuffling on the X chromosome is approximately 3.2 times higher than that on the autosomes. Conclusions The physical map will facilitate detailed genomic analysis of An. sinensis and contribute to understanding of the patterns and mechanisms of large-scale genome rearrangements in anopheline mosquitoes. |
format |
Article in Journal/Newspaper |
author |
Yun Wei Biao Cheng Guoding Zhu Danyu Shen Jiangtao Liang Cong Wang Jing Wang Jianxia Tang Jun Cao Igor V. Sharakhov Ai Xia |
author_facet |
Yun Wei Biao Cheng Guoding Zhu Danyu Shen Jiangtao Liang Cong Wang Jing Wang Jianxia Tang Jun Cao Igor V. Sharakhov Ai Xia |
author_sort |
Yun Wei |
title |
Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae |
title_short |
Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae |
title_full |
Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae |
title_fullStr |
Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae |
title_full_unstemmed |
Comparative physical genome mapping of malaria vectors Anopheles sinensis and Anopheles gambiae |
title_sort |
comparative physical genome mapping of malaria vectors anopheles sinensis and anopheles gambiae |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-1888-7 https://doaj.org/article/e39d2f736fec439f97391627ec18db62 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-10 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-1888-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1888-7 1475-2875 https://doaj.org/article/e39d2f736fec439f97391627ec18db62 |
op_doi |
https://doi.org/10.1186/s12936-017-1888-7 |
container_title |
Malaria Journal |
container_volume |
16 |
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1 |
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1766338258328879104 |