Early peritoneal immune response during Echinococcus granulosus establishment displays a biphasic behavior.
BACKGROUND: Cystic echinococcosis is a worldwide distributed helminth zoonosis caused by the larval stage of Echinococcus granulosus. Human secondary cystic echinococcosis is caused by dissemination of protoscoleces after accidental rupture of fertile cysts and is due to protoscoleces ability to dev...
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ftdoajarticles:oai:doaj.org/article:e38a217716224cf0b49a6c5b768f907b 2023-05-15T15:15:07+02:00 Early peritoneal immune response during Echinococcus granulosus establishment displays a biphasic behavior. Gustavo Mourglia-Ettlin Juan Martín Marqués José Alejandro Chabalgoity Sylvia Dematteis 2011-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001293 https://doaj.org/article/e38a217716224cf0b49a6c5b768f907b EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3166041?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001293 https://doaj.org/article/e38a217716224cf0b49a6c5b768f907b PLoS Neglected Tropical Diseases, Vol 5, Iss 8, p e1293 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001293 2022-12-31T03:31:30Z BACKGROUND: Cystic echinococcosis is a worldwide distributed helminth zoonosis caused by the larval stage of Echinococcus granulosus. Human secondary cystic echinococcosis is caused by dissemination of protoscoleces after accidental rupture of fertile cysts and is due to protoscoleces ability to develop into new metacestodes. In the experimental model of secondary cystic echinococcosis mice react against protoscoleces producing inefficient immune responses, allowing parasites to develop into cysts. Although the chronic phase of infection has been analyzed in depth, early immune responses at the site of infection establishment, e.g., peritoneal cavity, have not been well studied. Because during early stages of infection parasites are thought to be more susceptible to immune attack, this work focused on the study of cellular and molecular events triggered early in the peritoneal cavity of infected mice. PRINCIPAL FINDINGS: Data obtained showed disparate behaviors among subpopulations within the peritoneal lymphoid compartment. Regarding B cells, there is an active molecular process of plasma cell differentiation accompanied by significant local production of specific IgM and IgG2b antibodies. In addition, peritoneal NK cells showed a rapid increase with a significant percentage of activated cells. Peritoneal T cells showed a substantial increase, with predominance in CD4(+) T lymphocytes. There was also a local increase in Treg cells. Finally, cytokine response showed local biphasic kinetics: an early predominant induction of Th1-type cytokines (IFN-γ, IL-2 and IL-15), followed by a shift toward a Th2-type profile (IL-4, IL-5, IL-6, IL-10 and IL-13). CONCLUSIONS: Results reported here open new ways to investigate the involvement of immune effectors players in E. granulosus establishment, and also in the sequential promotion of Th1- toward Th2-type responses in experimental secondary cystic echinococcosis. These data would be relevant for designing rational therapies based on stimulation of effective responses and ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 8 e1293 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Gustavo Mourglia-Ettlin Juan Martín Marqués José Alejandro Chabalgoity Sylvia Dematteis Early peritoneal immune response during Echinococcus granulosus establishment displays a biphasic behavior. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Cystic echinococcosis is a worldwide distributed helminth zoonosis caused by the larval stage of Echinococcus granulosus. Human secondary cystic echinococcosis is caused by dissemination of protoscoleces after accidental rupture of fertile cysts and is due to protoscoleces ability to develop into new metacestodes. In the experimental model of secondary cystic echinococcosis mice react against protoscoleces producing inefficient immune responses, allowing parasites to develop into cysts. Although the chronic phase of infection has been analyzed in depth, early immune responses at the site of infection establishment, e.g., peritoneal cavity, have not been well studied. Because during early stages of infection parasites are thought to be more susceptible to immune attack, this work focused on the study of cellular and molecular events triggered early in the peritoneal cavity of infected mice. PRINCIPAL FINDINGS: Data obtained showed disparate behaviors among subpopulations within the peritoneal lymphoid compartment. Regarding B cells, there is an active molecular process of plasma cell differentiation accompanied by significant local production of specific IgM and IgG2b antibodies. In addition, peritoneal NK cells showed a rapid increase with a significant percentage of activated cells. Peritoneal T cells showed a substantial increase, with predominance in CD4(+) T lymphocytes. There was also a local increase in Treg cells. Finally, cytokine response showed local biphasic kinetics: an early predominant induction of Th1-type cytokines (IFN-γ, IL-2 and IL-15), followed by a shift toward a Th2-type profile (IL-4, IL-5, IL-6, IL-10 and IL-13). CONCLUSIONS: Results reported here open new ways to investigate the involvement of immune effectors players in E. granulosus establishment, and also in the sequential promotion of Th1- toward Th2-type responses in experimental secondary cystic echinococcosis. These data would be relevant for designing rational therapies based on stimulation of effective responses and ... |
format |
Article in Journal/Newspaper |
author |
Gustavo Mourglia-Ettlin Juan Martín Marqués José Alejandro Chabalgoity Sylvia Dematteis |
author_facet |
Gustavo Mourglia-Ettlin Juan Martín Marqués José Alejandro Chabalgoity Sylvia Dematteis |
author_sort |
Gustavo Mourglia-Ettlin |
title |
Early peritoneal immune response during Echinococcus granulosus establishment displays a biphasic behavior. |
title_short |
Early peritoneal immune response during Echinococcus granulosus establishment displays a biphasic behavior. |
title_full |
Early peritoneal immune response during Echinococcus granulosus establishment displays a biphasic behavior. |
title_fullStr |
Early peritoneal immune response during Echinococcus granulosus establishment displays a biphasic behavior. |
title_full_unstemmed |
Early peritoneal immune response during Echinococcus granulosus establishment displays a biphasic behavior. |
title_sort |
early peritoneal immune response during echinococcus granulosus establishment displays a biphasic behavior. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001293 https://doaj.org/article/e38a217716224cf0b49a6c5b768f907b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 8, p e1293 (2011) |
op_relation |
http://europepmc.org/articles/PMC3166041?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001293 https://doaj.org/article/e38a217716224cf0b49a6c5b768f907b |
op_doi |
https://doi.org/10.1371/journal.pntd.0001293 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
8 |
container_start_page |
e1293 |
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1766345499489599488 |