Control of pyrethroid-resistant Chagas disease vectors with entomopathogenic fungi.
BACKGROUND: Triatoma infestans-mediated transmission of Tripanosoma cruzi, the causative agent of Chagas disease, remains as a major health issue in southern South America. Key factors of T. infestans prevalence in specific areas of the geographic Gran Chaco region-which extends through northern Arg...
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ftdoajarticles:oai:doaj.org/article:e3711d8304cd4a50996d9d332d425a56 2023-05-15T15:12:13+02:00 Control of pyrethroid-resistant Chagas disease vectors with entomopathogenic fungi. Nicolás Pedrini Sergio J Mijailovsky Juan R Girotti Raúl Stariolo Rubén M Cardozo Alberto Gentile M Patricia Juárez 2009-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000434 https://doaj.org/article/e3711d8304cd4a50996d9d332d425a56 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2674565?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000434 https://doaj.org/article/e3711d8304cd4a50996d9d332d425a56 PLoS Neglected Tropical Diseases, Vol 3, Iss 5, p e434 (2009) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2009 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000434 2022-12-31T13:19:24Z BACKGROUND: Triatoma infestans-mediated transmission of Tripanosoma cruzi, the causative agent of Chagas disease, remains as a major health issue in southern South America. Key factors of T. infestans prevalence in specific areas of the geographic Gran Chaco region-which extends through northern Argentina, Bolivia, and Paraguay-are both recurrent reinfestations after insecticide spraying and emerging pyrethroid-resistance over the past ten years. Among alternative control tools, the pathogenicity of entomopathogenic fungi against triatomines is already known; furthermore, these fungi have the ability to fully degrade hydrocarbons from T. infestans cuticle and to utilize them as fuel and for incorporation into cellular components. METHODOLOGY AND FINDINGS: Here we provide evidence of resistance-related cuticle differences; capillary gas chromatography coupled to mass spectrometry analyses revealed that pyrethroid-resistant bugs have significantly larger amounts of surface hydrocarbons, peaking 56.2+/-6.4% higher than susceptible specimens. Also, a thicker cuticle was detected by scanning electron microscopy (32.1+/-5.9 microm and 17.8+/-5.4 microm for pyrethroid-resistant and pyrethroid-susceptible, respectively). In laboratory bioassays, we showed that the virulence of the entomopathogenic fungi Beauveria bassiana against T. infestans was significantly enhanced after fungal adaptation to grow on a medium containing insect-like hydrocarbons as the carbon source, regardless of bug susceptibility to pyrethroids. We designed an attraction-infection trap based on manipulating T. infestans behavior in order to facilitate close contact with B. bassiana. Field assays performed in rural village houses infested with pyrethroid-resistant insects showed 52.4% bug mortality. Using available mathematical models, we predicted that further fungal applications could eventually halt infection transmission. CONCLUSIONS: This low cost, low tech, ecologically friendly methodology could help in controlling the spread of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Argentina Chaco ENVELOPE(-60.583,-60.583,-63.033,-63.033) PLoS Neglected Tropical Diseases 3 5 e434 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Nicolás Pedrini Sergio J Mijailovsky Juan R Girotti Raúl Stariolo Rubén M Cardozo Alberto Gentile M Patricia Juárez Control of pyrethroid-resistant Chagas disease vectors with entomopathogenic fungi. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Triatoma infestans-mediated transmission of Tripanosoma cruzi, the causative agent of Chagas disease, remains as a major health issue in southern South America. Key factors of T. infestans prevalence in specific areas of the geographic Gran Chaco region-which extends through northern Argentina, Bolivia, and Paraguay-are both recurrent reinfestations after insecticide spraying and emerging pyrethroid-resistance over the past ten years. Among alternative control tools, the pathogenicity of entomopathogenic fungi against triatomines is already known; furthermore, these fungi have the ability to fully degrade hydrocarbons from T. infestans cuticle and to utilize them as fuel and for incorporation into cellular components. METHODOLOGY AND FINDINGS: Here we provide evidence of resistance-related cuticle differences; capillary gas chromatography coupled to mass spectrometry analyses revealed that pyrethroid-resistant bugs have significantly larger amounts of surface hydrocarbons, peaking 56.2+/-6.4% higher than susceptible specimens. Also, a thicker cuticle was detected by scanning electron microscopy (32.1+/-5.9 microm and 17.8+/-5.4 microm for pyrethroid-resistant and pyrethroid-susceptible, respectively). In laboratory bioassays, we showed that the virulence of the entomopathogenic fungi Beauveria bassiana against T. infestans was significantly enhanced after fungal adaptation to grow on a medium containing insect-like hydrocarbons as the carbon source, regardless of bug susceptibility to pyrethroids. We designed an attraction-infection trap based on manipulating T. infestans behavior in order to facilitate close contact with B. bassiana. Field assays performed in rural village houses infested with pyrethroid-resistant insects showed 52.4% bug mortality. Using available mathematical models, we predicted that further fungal applications could eventually halt infection transmission. CONCLUSIONS: This low cost, low tech, ecologically friendly methodology could help in controlling the spread of ... |
format |
Article in Journal/Newspaper |
author |
Nicolás Pedrini Sergio J Mijailovsky Juan R Girotti Raúl Stariolo Rubén M Cardozo Alberto Gentile M Patricia Juárez |
author_facet |
Nicolás Pedrini Sergio J Mijailovsky Juan R Girotti Raúl Stariolo Rubén M Cardozo Alberto Gentile M Patricia Juárez |
author_sort |
Nicolás Pedrini |
title |
Control of pyrethroid-resistant Chagas disease vectors with entomopathogenic fungi. |
title_short |
Control of pyrethroid-resistant Chagas disease vectors with entomopathogenic fungi. |
title_full |
Control of pyrethroid-resistant Chagas disease vectors with entomopathogenic fungi. |
title_fullStr |
Control of pyrethroid-resistant Chagas disease vectors with entomopathogenic fungi. |
title_full_unstemmed |
Control of pyrethroid-resistant Chagas disease vectors with entomopathogenic fungi. |
title_sort |
control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2009 |
url |
https://doi.org/10.1371/journal.pntd.0000434 https://doaj.org/article/e3711d8304cd4a50996d9d332d425a56 |
long_lat |
ENVELOPE(-60.583,-60.583,-63.033,-63.033) |
geographic |
Arctic Argentina Chaco |
geographic_facet |
Arctic Argentina Chaco |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 3, Iss 5, p e434 (2009) |
op_relation |
http://europepmc.org/articles/PMC2674565?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000434 https://doaj.org/article/e3711d8304cd4a50996d9d332d425a56 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000434 |
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PLoS Neglected Tropical Diseases |
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5 |
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e434 |
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