South African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory.
Background In August 2014, the National Institute for Communicable Diseases (NICD) in South Africa established a modular high-biosafety field Ebola diagnostic laboratory (SA FEDL) near Freetown, Sierra Leone in response to the rapidly increasing number of Ebola virus disease (EVD) cases. Methods and...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:e360ae8bd9ec4bc39a30d9fb12803322 2023-05-15T15:16:17+02:00 South African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory. Janusz T Paweska Petrus Jansen van Vuren Gunther H Meier Chantel le Roux Ousman S Conteh Alan Kemp Cardia Fourie Prabha Naidoo Serisha Naicker Phumza Ohaebosim Nadia Storm Orienka Hellferscee Lisa K Ming Sun Busisiwe Mogodi Nishi Prabdial-Sing Desiree du Plessis Deidre Greyling Shayne Loubser Mark Goosen Stewart D McCulloch Terence P Scott Alexandra Moerdyk Wesley Dlamini Kelfala Konneh Idrissa L Kamara Dauda Sowa Samuel Sorie Brima Kargbo Shabir A Madhi 2017-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005665 https://doaj.org/article/e360ae8bd9ec4bc39a30d9fb12803322 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0005665 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005665 https://doaj.org/article/e360ae8bd9ec4bc39a30d9fb12803322 PLoS Neglected Tropical Diseases, Vol 11, Iss 6, p e0005665 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005665 2022-12-31T10:05:03Z Background In August 2014, the National Institute for Communicable Diseases (NICD) in South Africa established a modular high-biosafety field Ebola diagnostic laboratory (SA FEDL) near Freetown, Sierra Leone in response to the rapidly increasing number of Ebola virus disease (EVD) cases. Methods and findings The SA FEDL operated in the Western Area of Sierra Leone, which remained a "hotspot" of the EVD epidemic for months. The FEDL was the only diagnostic capacity available to respond to the overwhelming demand for rapid EVD laboratory diagnosis for several weeks in the initial stages of the EVD crisis in the capital of Sierra Leone. Furthermore, the NICD set out to establish local capacity amongst Sierra Leonean nationals in all aspects of the FEDL functions from the outset. This led to the successful hand-over of the FEDL to the Sierra Leone Ministry of Health and Sanitation in March 2015. Between 25 August 2014 and 22 June 2016, the laboratory tested 11,250 specimens mostly from the Western Urban and Western Rural regions of Sierra Leone, of which 2,379 (21.14%) tested positive for Ebola virus RNA. Conclusions The bio-safety standards and the portability of the SA FEDL, offered a cost-effective and practical alternative for the rapid deployment of a field-operated high biocontainment facility. The SA FEDL teams demonstrated that it is highly beneficial to train the national staff in the course of formidable disease outbreak and accomplished their full integration into all operational and diagnostic aspects of the laboratory. This initiative contributed to the international efforts in bringing the EVD outbreak under control in Sierra Leone, as well as capacitating local African scientists and technologists to respond to diagnostic needs that might be required in future outbreaks of highly contagious pathogens. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 6 e0005665 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Janusz T Paweska Petrus Jansen van Vuren Gunther H Meier Chantel le Roux Ousman S Conteh Alan Kemp Cardia Fourie Prabha Naidoo Serisha Naicker Phumza Ohaebosim Nadia Storm Orienka Hellferscee Lisa K Ming Sun Busisiwe Mogodi Nishi Prabdial-Sing Desiree du Plessis Deidre Greyling Shayne Loubser Mark Goosen Stewart D McCulloch Terence P Scott Alexandra Moerdyk Wesley Dlamini Kelfala Konneh Idrissa L Kamara Dauda Sowa Samuel Sorie Brima Kargbo Shabir A Madhi South African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background In August 2014, the National Institute for Communicable Diseases (NICD) in South Africa established a modular high-biosafety field Ebola diagnostic laboratory (SA FEDL) near Freetown, Sierra Leone in response to the rapidly increasing number of Ebola virus disease (EVD) cases. Methods and findings The SA FEDL operated in the Western Area of Sierra Leone, which remained a "hotspot" of the EVD epidemic for months. The FEDL was the only diagnostic capacity available to respond to the overwhelming demand for rapid EVD laboratory diagnosis for several weeks in the initial stages of the EVD crisis in the capital of Sierra Leone. Furthermore, the NICD set out to establish local capacity amongst Sierra Leonean nationals in all aspects of the FEDL functions from the outset. This led to the successful hand-over of the FEDL to the Sierra Leone Ministry of Health and Sanitation in March 2015. Between 25 August 2014 and 22 June 2016, the laboratory tested 11,250 specimens mostly from the Western Urban and Western Rural regions of Sierra Leone, of which 2,379 (21.14%) tested positive for Ebola virus RNA. Conclusions The bio-safety standards and the portability of the SA FEDL, offered a cost-effective and practical alternative for the rapid deployment of a field-operated high biocontainment facility. The SA FEDL teams demonstrated that it is highly beneficial to train the national staff in the course of formidable disease outbreak and accomplished their full integration into all operational and diagnostic aspects of the laboratory. This initiative contributed to the international efforts in bringing the EVD outbreak under control in Sierra Leone, as well as capacitating local African scientists and technologists to respond to diagnostic needs that might be required in future outbreaks of highly contagious pathogens. |
format |
Article in Journal/Newspaper |
author |
Janusz T Paweska Petrus Jansen van Vuren Gunther H Meier Chantel le Roux Ousman S Conteh Alan Kemp Cardia Fourie Prabha Naidoo Serisha Naicker Phumza Ohaebosim Nadia Storm Orienka Hellferscee Lisa K Ming Sun Busisiwe Mogodi Nishi Prabdial-Sing Desiree du Plessis Deidre Greyling Shayne Loubser Mark Goosen Stewart D McCulloch Terence P Scott Alexandra Moerdyk Wesley Dlamini Kelfala Konneh Idrissa L Kamara Dauda Sowa Samuel Sorie Brima Kargbo Shabir A Madhi |
author_facet |
Janusz T Paweska Petrus Jansen van Vuren Gunther H Meier Chantel le Roux Ousman S Conteh Alan Kemp Cardia Fourie Prabha Naidoo Serisha Naicker Phumza Ohaebosim Nadia Storm Orienka Hellferscee Lisa K Ming Sun Busisiwe Mogodi Nishi Prabdial-Sing Desiree du Plessis Deidre Greyling Shayne Loubser Mark Goosen Stewart D McCulloch Terence P Scott Alexandra Moerdyk Wesley Dlamini Kelfala Konneh Idrissa L Kamara Dauda Sowa Samuel Sorie Brima Kargbo Shabir A Madhi |
author_sort |
Janusz T Paweska |
title |
South African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory. |
title_short |
South African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory. |
title_full |
South African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory. |
title_fullStr |
South African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory. |
title_full_unstemmed |
South African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory. |
title_sort |
south african ebola diagnostic response in sierra leone: a modular high biosafety field laboratory. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005665 https://doaj.org/article/e360ae8bd9ec4bc39a30d9fb12803322 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 6, p e0005665 (2017) |
op_relation |
https://doi.org/10.1371/journal.pntd.0005665 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005665 https://doaj.org/article/e360ae8bd9ec4bc39a30d9fb12803322 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005665 |
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PLOS Neglected Tropical Diseases |
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11 |
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6 |
container_start_page |
e0005665 |
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