Morphological and Molecular Characterization of Paragonimus Species Isolated from Freshwater Crabs in Southern Yunnan, China
Paragonimus species are highly prevalent in various regions of China. The study’s objective is to isolate and identify Paragonimus from natural habitats and compare the phylogenetic diversity of Paragonimus in southern Yunnan province, China. Metacercariae of Paragonimus was isolated from crabs, and...
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ftdoajarticles:oai:doaj.org/article:e2e3cc378a34456eb3f6ea00b952f67a 2023-05-15T15:13:49+02:00 Morphological and Molecular Characterization of Paragonimus Species Isolated from Freshwater Crabs in Southern Yunnan, China Qiu-Hong Shu Yang Yang Miao-Miao Wang Shu-De Li Ming Tian Wen-Wei Bai Yong Meng Shu-Mei-Qi He Wen-Lin Wang 2021-01-01T00:00:00Z https://doi.org/10.1155/2021/5646291 https://doaj.org/article/e2e3cc378a34456eb3f6ea00b952f67a EN eng Hindawi Limited http://dx.doi.org/10.1155/2021/5646291 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2021/5646291 https://doaj.org/article/e2e3cc378a34456eb3f6ea00b952f67a Journal of Tropical Medicine, Vol 2021 (2021) Arctic medicine. Tropical medicine RC955-962 article 2021 ftdoajarticles https://doi.org/10.1155/2021/5646291 2022-12-31T16:32:44Z Paragonimus species are highly prevalent in various regions of China. The study’s objective is to isolate and identify Paragonimus from natural habitats and compare the phylogenetic diversity of Paragonimus in southern Yunnan province, China. Metacercariae of Paragonimus was isolated from crabs, and morphologic identification was performed by microscopy. Metacercariae were injected into experimental Paragonimus free Sprague Dawley rats. After 114 days, adult worms and eggs were isolated from multiple organs. Morphologic identification confirmed the initial identification. DNA was extracted from 5 adult worms, and molecular characterization was performed by amplification and sequencing of CO1 and ITS2 regions, followed by phylogenetic analysis. Out of 447 crabs captured, 186 crabs were found to be infected. A total of 4 species of Paragonimus was observed from naturally infected crabs. Paragonimus microrchis (2), Paragonimus heterotremus (1), Paragonimus proliferus (1), and Paragonimus skrjabini (1) were isolated and identified. A total of 32 sequences were downloaded from the National Center for Biotechnology Information, and 5 sequences generated in the study were used for phylogenetic analysis. In the phylogenetic tree of the CO1 gene, Paragonimus proliferus, Paragonimus heterotremus, and Paragonimus skrjabini were clustered with the same species, and the confidence values of their branches were >95%. A congruent phylogenetic relationship was observed with the ITS2 phylogenetic tree. In the phylogenetic tree constructed with the combined dataset of CO1 and ITS2 datasets, Paragonimus proliferus, Paragonimus heterotremus, and Paragonimus skrjabini clustered with the same species, and their branch confidence values were >94%. Paragonimus microrchis clustered with Paragonimus bangkokensis in both datasets. Phylogenetic analysis revealed robustness of the double loci method as against the single-locus method with either CO1 or ITS2 alone. Paragonimus species isolated from the southern Yunnan province, China, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2021 1 11 |
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Arctic medicine. Tropical medicine RC955-962 |
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Arctic medicine. Tropical medicine RC955-962 Qiu-Hong Shu Yang Yang Miao-Miao Wang Shu-De Li Ming Tian Wen-Wei Bai Yong Meng Shu-Mei-Qi He Wen-Lin Wang Morphological and Molecular Characterization of Paragonimus Species Isolated from Freshwater Crabs in Southern Yunnan, China |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
Paragonimus species are highly prevalent in various regions of China. The study’s objective is to isolate and identify Paragonimus from natural habitats and compare the phylogenetic diversity of Paragonimus in southern Yunnan province, China. Metacercariae of Paragonimus was isolated from crabs, and morphologic identification was performed by microscopy. Metacercariae were injected into experimental Paragonimus free Sprague Dawley rats. After 114 days, adult worms and eggs were isolated from multiple organs. Morphologic identification confirmed the initial identification. DNA was extracted from 5 adult worms, and molecular characterization was performed by amplification and sequencing of CO1 and ITS2 regions, followed by phylogenetic analysis. Out of 447 crabs captured, 186 crabs were found to be infected. A total of 4 species of Paragonimus was observed from naturally infected crabs. Paragonimus microrchis (2), Paragonimus heterotremus (1), Paragonimus proliferus (1), and Paragonimus skrjabini (1) were isolated and identified. A total of 32 sequences were downloaded from the National Center for Biotechnology Information, and 5 sequences generated in the study were used for phylogenetic analysis. In the phylogenetic tree of the CO1 gene, Paragonimus proliferus, Paragonimus heterotremus, and Paragonimus skrjabini were clustered with the same species, and the confidence values of their branches were >95%. A congruent phylogenetic relationship was observed with the ITS2 phylogenetic tree. In the phylogenetic tree constructed with the combined dataset of CO1 and ITS2 datasets, Paragonimus proliferus, Paragonimus heterotremus, and Paragonimus skrjabini clustered with the same species, and their branch confidence values were >94%. Paragonimus microrchis clustered with Paragonimus bangkokensis in both datasets. Phylogenetic analysis revealed robustness of the double loci method as against the single-locus method with either CO1 or ITS2 alone. Paragonimus species isolated from the southern Yunnan province, China, ... |
format |
Article in Journal/Newspaper |
author |
Qiu-Hong Shu Yang Yang Miao-Miao Wang Shu-De Li Ming Tian Wen-Wei Bai Yong Meng Shu-Mei-Qi He Wen-Lin Wang |
author_facet |
Qiu-Hong Shu Yang Yang Miao-Miao Wang Shu-De Li Ming Tian Wen-Wei Bai Yong Meng Shu-Mei-Qi He Wen-Lin Wang |
author_sort |
Qiu-Hong Shu |
title |
Morphological and Molecular Characterization of Paragonimus Species Isolated from Freshwater Crabs in Southern Yunnan, China |
title_short |
Morphological and Molecular Characterization of Paragonimus Species Isolated from Freshwater Crabs in Southern Yunnan, China |
title_full |
Morphological and Molecular Characterization of Paragonimus Species Isolated from Freshwater Crabs in Southern Yunnan, China |
title_fullStr |
Morphological and Molecular Characterization of Paragonimus Species Isolated from Freshwater Crabs in Southern Yunnan, China |
title_full_unstemmed |
Morphological and Molecular Characterization of Paragonimus Species Isolated from Freshwater Crabs in Southern Yunnan, China |
title_sort |
morphological and molecular characterization of paragonimus species isolated from freshwater crabs in southern yunnan, china |
publisher |
Hindawi Limited |
publishDate |
2021 |
url |
https://doi.org/10.1155/2021/5646291 https://doaj.org/article/e2e3cc378a34456eb3f6ea00b952f67a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2021 (2021) |
op_relation |
http://dx.doi.org/10.1155/2021/5646291 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2021/5646291 https://doaj.org/article/e2e3cc378a34456eb3f6ea00b952f67a |
op_doi |
https://doi.org/10.1155/2021/5646291 |
container_title |
Journal of Tropical Medicine |
container_volume |
2021 |
container_start_page |
1 |
op_container_end_page |
11 |
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1766344335870132224 |