Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera
Highly negative δ13C values in fossil foraminifera from methane cold seeps have been proposed to reflect episodes of methane release from gas hydrate dissociation or free gas reservoirs triggered by climatic changes in the past. Because most studies on live foraminifera are based on the presence of...
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ftdoajarticles:oai:doaj.org/article:e2d119c76702450ebb014ac38314defd 2023-05-15T14:59:49+02:00 Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera Katarzyna Melaniuk 2021-03-01T00:00:00Z https://doi.org/10.3389/fmars.2021.587748 https://doaj.org/article/e2d119c76702450ebb014ac38314defd EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2021.587748/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2021.587748 https://doaj.org/article/e2d119c76702450ebb014ac38314defd Frontiers in Marine Science, Vol 8 (2021) CellTrackerTM Green CMFDA Rose Bengal gas hydrate Vestnesa Ridge Storfjordrenna cold seep Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2021 ftdoajarticles https://doi.org/10.3389/fmars.2021.587748 2022-12-31T06:41:36Z Highly negative δ13C values in fossil foraminifera from methane cold seeps have been proposed to reflect episodes of methane release from gas hydrate dissociation or free gas reservoirs triggered by climatic changes in the past. Because most studies on live foraminifera are based on the presence of Rose Bengal staining, that colors the cytoplasm of both live and recently dead individuals it remains unclear if, and to what extent live foraminifera incorporate methane-derived carbon during biomineralization, or whether the isotopic signature is mostly affected by authigenic overgrowth. In this paper, modern foraminiferal assemblages from a gas hydrate province Vestnesa Ridge (∼1,200 m water depth, northeastern Fram Strait) and from Storfjordrenna (∼400 m water depth in the western Barents Sea) is presented. By using the fluorescent viability assays CellTrackerTM Green (CTG) CMFDA and CellHunt Green (CHG) together with conventional Rose Bengal, it was possible to examine live and recently dead foraminifera separately. Metabolically active foraminifera were shown to inhabit methane-enriched sediments at both investigated locations. The benthic foraminiferal faunas were dominated by common Arctic species such as Melonis barleeanus, Cassidulina neoteretis, and Nonionellina labradorica. The combined usage of the fluorescence probe and Rose Bengal revealed only minor shifts in species compositions and differences in ratios between live and recently dead foraminifera from Storfjordrenna. There was no clear evidence that methane significantly affected the δ13C signature of the calcite of living specimens. Article in Journal/Newspaper Arctic Barents Sea Foraminifera* Fram Strait Storfjordrenna Directory of Open Access Journals: DOAJ Articles Arctic Barents Sea Storfjordrenna ENVELOPE(17.000,17.000,76.000,76.000) Frontiers in Marine Science 8 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
CellTrackerTM Green CMFDA Rose Bengal gas hydrate Vestnesa Ridge Storfjordrenna cold seep Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
CellTrackerTM Green CMFDA Rose Bengal gas hydrate Vestnesa Ridge Storfjordrenna cold seep Science Q General. Including nature conservation geographical distribution QH1-199.5 Katarzyna Melaniuk Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera |
topic_facet |
CellTrackerTM Green CMFDA Rose Bengal gas hydrate Vestnesa Ridge Storfjordrenna cold seep Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
Highly negative δ13C values in fossil foraminifera from methane cold seeps have been proposed to reflect episodes of methane release from gas hydrate dissociation or free gas reservoirs triggered by climatic changes in the past. Because most studies on live foraminifera are based on the presence of Rose Bengal staining, that colors the cytoplasm of both live and recently dead individuals it remains unclear if, and to what extent live foraminifera incorporate methane-derived carbon during biomineralization, or whether the isotopic signature is mostly affected by authigenic overgrowth. In this paper, modern foraminiferal assemblages from a gas hydrate province Vestnesa Ridge (∼1,200 m water depth, northeastern Fram Strait) and from Storfjordrenna (∼400 m water depth in the western Barents Sea) is presented. By using the fluorescent viability assays CellTrackerTM Green (CTG) CMFDA and CellHunt Green (CHG) together with conventional Rose Bengal, it was possible to examine live and recently dead foraminifera separately. Metabolically active foraminifera were shown to inhabit methane-enriched sediments at both investigated locations. The benthic foraminiferal faunas were dominated by common Arctic species such as Melonis barleeanus, Cassidulina neoteretis, and Nonionellina labradorica. The combined usage of the fluorescence probe and Rose Bengal revealed only minor shifts in species compositions and differences in ratios between live and recently dead foraminifera from Storfjordrenna. There was no clear evidence that methane significantly affected the δ13C signature of the calcite of living specimens. |
format |
Article in Journal/Newspaper |
author |
Katarzyna Melaniuk |
author_facet |
Katarzyna Melaniuk |
author_sort |
Katarzyna Melaniuk |
title |
Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera |
title_short |
Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera |
title_full |
Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera |
title_fullStr |
Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera |
title_full_unstemmed |
Effectiveness of Fluorescent Viability Assays in Studies of Arctic Cold Seep Foraminifera |
title_sort |
effectiveness of fluorescent viability assays in studies of arctic cold seep foraminifera |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmars.2021.587748 https://doaj.org/article/e2d119c76702450ebb014ac38314defd |
long_lat |
ENVELOPE(17.000,17.000,76.000,76.000) |
geographic |
Arctic Barents Sea Storfjordrenna |
geographic_facet |
Arctic Barents Sea Storfjordrenna |
genre |
Arctic Barents Sea Foraminifera* Fram Strait Storfjordrenna |
genre_facet |
Arctic Barents Sea Foraminifera* Fram Strait Storfjordrenna |
op_source |
Frontiers in Marine Science, Vol 8 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2021.587748/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2021.587748 https://doaj.org/article/e2d119c76702450ebb014ac38314defd |
op_doi |
https://doi.org/10.3389/fmars.2021.587748 |
container_title |
Frontiers in Marine Science |
container_volume |
8 |
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1766331940298817536 |