Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica
With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the...
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ftdoajarticles:oai:doaj.org/article:e2b2614a9797462cac84bc8768ae82e4 2023-05-15T13:30:38+02:00 Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica Salihu Ibrahim Khalilah Abdul Khalil Khadijah Nabilah Mohd Zahri Claudio Gomez-Fuentes Peter Convey Azham Zulkarnain Suriana Sabri Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad 2020-08-01T00:00:00Z https://doi.org/10.3390/molecules25173878 https://doaj.org/article/e2b2614a9797462cac84bc8768ae82e4 EN eng MDPI AG https://www.mdpi.com/1420-3049/25/17/3878 https://doaj.org/toc/1420-3049 doi:10.3390/molecules25173878 1420-3049 https://doaj.org/article/e2b2614a9797462cac84bc8768ae82e4 Molecules, Vol 25, Iss 3878, p 3878 (2020) Antarctica biosurfactants canola oil kinetics haldane modelling Organic chemistry QD241-441 article 2020 ftdoajarticles https://doi.org/10.3390/molecules25173878 2022-12-31T03:50:18Z With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, Rhodococcus erythropolis strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and “MATH” assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate ( q max ), concentration of substrate at the half maximum specific growth rate, K s % ( v/v ) and the inhibition constant K i % ( v/v ), with values of 0.142 h −1 , 7.743% ( v/v ) and 0.399% ( v/v ), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to “in field” bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Molecules 25 17 3878 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctica biosurfactants canola oil kinetics haldane modelling Organic chemistry QD241-441 |
spellingShingle |
Antarctica biosurfactants canola oil kinetics haldane modelling Organic chemistry QD241-441 Salihu Ibrahim Khalilah Abdul Khalil Khadijah Nabilah Mohd Zahri Claudio Gomez-Fuentes Peter Convey Azham Zulkarnain Suriana Sabri Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
topic_facet |
Antarctica biosurfactants canola oil kinetics haldane modelling Organic chemistry QD241-441 |
description |
With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, Rhodococcus erythropolis strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and “MATH” assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate ( q max ), concentration of substrate at the half maximum specific growth rate, K s % ( v/v ) and the inhibition constant K i % ( v/v ), with values of 0.142 h −1 , 7.743% ( v/v ) and 0.399% ( v/v ), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to “in field” bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions. |
format |
Article in Journal/Newspaper |
author |
Salihu Ibrahim Khalilah Abdul Khalil Khadijah Nabilah Mohd Zahri Claudio Gomez-Fuentes Peter Convey Azham Zulkarnain Suriana Sabri Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad |
author_facet |
Salihu Ibrahim Khalilah Abdul Khalil Khadijah Nabilah Mohd Zahri Claudio Gomez-Fuentes Peter Convey Azham Zulkarnain Suriana Sabri Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad |
author_sort |
Salihu Ibrahim |
title |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_short |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_full |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_fullStr |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_full_unstemmed |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_sort |
biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium rhodococcus erythropolis aq5-07 from antarctica |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/molecules25173878 https://doaj.org/article/e2b2614a9797462cac84bc8768ae82e4 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Molecules, Vol 25, Iss 3878, p 3878 (2020) |
op_relation |
https://www.mdpi.com/1420-3049/25/17/3878 https://doaj.org/toc/1420-3049 doi:10.3390/molecules25173878 1420-3049 https://doaj.org/article/e2b2614a9797462cac84bc8768ae82e4 |
op_doi |
https://doi.org/10.3390/molecules25173878 |
container_title |
Molecules |
container_volume |
25 |
container_issue |
17 |
container_start_page |
3878 |
_version_ |
1766011058420449280 |