Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis.
INTRODUCTION:Cutaneous leishmaniasis (CL) due to L.braziliensis infection is characterized by a strong inflammatory response with high levels of TNF and ulcer development. Less attention has been given to the role of mononuclear phagocytes to this process. Monocytes constitute a heterogeneous popula...
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ftdoajarticles:oai:doaj.org/article:e28e135f1ca6435c8f114cd50d44771f 2023-05-15T15:08:39+02:00 Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis. Taís M Campos Sara T Passos Fernanda O Novais Daniel P Beiting Rúbia S Costa Adriano Queiroz David Mosser Phillip Scott Edgar M Carvalho Lucas P Carvalho 2014-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003282 https://doaj.org/article/e28e135f1ca6435c8f114cd50d44771f EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4230914?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003282 https://doaj.org/article/e28e135f1ca6435c8f114cd50d44771f PLoS Neglected Tropical Diseases, Vol 8, Iss 11, p e3282 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003282 2022-12-31T00:49:22Z INTRODUCTION:Cutaneous leishmaniasis (CL) due to L.braziliensis infection is characterized by a strong inflammatory response with high levels of TNF and ulcer development. Less attention has been given to the role of mononuclear phagocytes to this process. Monocytes constitute a heterogeneous population subdivided into classical, intermediate and non-classical, and are known to migrate to inflammatory sites and secrete inflammatory mediators. TNF participates in the induction of matrix metalloproteinases (MMPs). MMP-9 is an enzyme that degrades basal membrane and its activity is controlled by the tissue inhibitor of metalloproteinase. METHODS:Mononuclear cells were obtained from ex-vivo labeling sub-populations of monocytes and MMP-9, and the frequency was determined by flow cytometry. Culture was performed during 72 hours, stimulating the cells with SLA, levels of MMP-9 and TIMP-1 in the supernatants were determined by ELISA. RESULTS:We observed that cells from CL lesions secrete high amounts of MMP-9 when compared to healthy subjects. Although MMP-9 was produced by monocytes, non-classical ones were the main source of this enzyme. We also observed that TNF produced in high level during CL contributes to MMP-9 production. CONCLUSIONS:These observations emphasize the role of monocytes, TNF and MMP-9 in the pathogenesis of L. braziliensis infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 11 e3282 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Taís M Campos Sara T Passos Fernanda O Novais Daniel P Beiting Rúbia S Costa Adriano Queiroz David Mosser Phillip Scott Edgar M Carvalho Lucas P Carvalho Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
INTRODUCTION:Cutaneous leishmaniasis (CL) due to L.braziliensis infection is characterized by a strong inflammatory response with high levels of TNF and ulcer development. Less attention has been given to the role of mononuclear phagocytes to this process. Monocytes constitute a heterogeneous population subdivided into classical, intermediate and non-classical, and are known to migrate to inflammatory sites and secrete inflammatory mediators. TNF participates in the induction of matrix metalloproteinases (MMPs). MMP-9 is an enzyme that degrades basal membrane and its activity is controlled by the tissue inhibitor of metalloproteinase. METHODS:Mononuclear cells were obtained from ex-vivo labeling sub-populations of monocytes and MMP-9, and the frequency was determined by flow cytometry. Culture was performed during 72 hours, stimulating the cells with SLA, levels of MMP-9 and TIMP-1 in the supernatants were determined by ELISA. RESULTS:We observed that cells from CL lesions secrete high amounts of MMP-9 when compared to healthy subjects. Although MMP-9 was produced by monocytes, non-classical ones were the main source of this enzyme. We also observed that TNF produced in high level during CL contributes to MMP-9 production. CONCLUSIONS:These observations emphasize the role of monocytes, TNF and MMP-9 in the pathogenesis of L. braziliensis infection. |
format |
Article in Journal/Newspaper |
author |
Taís M Campos Sara T Passos Fernanda O Novais Daniel P Beiting Rúbia S Costa Adriano Queiroz David Mosser Phillip Scott Edgar M Carvalho Lucas P Carvalho |
author_facet |
Taís M Campos Sara T Passos Fernanda O Novais Daniel P Beiting Rúbia S Costa Adriano Queiroz David Mosser Phillip Scott Edgar M Carvalho Lucas P Carvalho |
author_sort |
Taís M Campos |
title |
Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis. |
title_short |
Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis. |
title_full |
Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis. |
title_fullStr |
Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis. |
title_full_unstemmed |
Matrix metalloproteinase 9 production by monocytes is enhanced by TNF and participates in the pathology of human cutaneous Leishmaniasis. |
title_sort |
matrix metalloproteinase 9 production by monocytes is enhanced by tnf and participates in the pathology of human cutaneous leishmaniasis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0003282 https://doaj.org/article/e28e135f1ca6435c8f114cd50d44771f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 11, p e3282 (2014) |
op_relation |
http://europepmc.org/articles/PMC4230914?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003282 https://doaj.org/article/e28e135f1ca6435c8f114cd50d44771f |
op_doi |
https://doi.org/10.1371/journal.pntd.0003282 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
11 |
container_start_page |
e3282 |
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1766339969004077056 |