Corn gluten meal induces enteritis and decreases intestinal immunity and antioxidant capacity in turbot (Scophthalmus maximus) at high supplementation levels.
Corn gluten meal (CGM) is an important alternative protein source in aquafeed production. However, in turbot (Scophthalmus maximus), CGM could not be effectively utilized because of its low digestibility, the reason for which is still unclear. The purpose of the present study was to investigate and...
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ftdoajarticles:oai:doaj.org/article:e25c3ccf6c1a48408293e05076e062f3 2023-05-15T18:15:45+02:00 Corn gluten meal induces enteritis and decreases intestinal immunity and antioxidant capacity in turbot (Scophthalmus maximus) at high supplementation levels. Nan Bai Min Gu Mingjie Liu Qian Jia Shihui Pan Zhiyu Zhang 2019-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0213867 https://doaj.org/article/e25c3ccf6c1a48408293e05076e062f3 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0213867 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0213867 https://doaj.org/article/e25c3ccf6c1a48408293e05076e062f3 PLoS ONE, Vol 14, Iss 3, p e0213867 (2019) Medicine R Science Q article 2019 ftdoajarticles https://doi.org/10.1371/journal.pone.0213867 2022-12-31T05:54:00Z Corn gluten meal (CGM) is an important alternative protein source in aquafeed production. However, in turbot (Scophthalmus maximus), CGM could not be effectively utilized because of its low digestibility, the reason for which is still unclear. The purpose of the present study was to investigate and elucidate the cause for the poor utilization of CGM by turbot from the view of gut health. An 8-week feeding trial was conducted with turbot individuals (initial body weight 11.4 ± 0.2 g), which were fed with one of four isonitrogenous and isolipidic diets formulated to include 0%, 21.2%, 31.8%, and 42.6% CGM to progressively replace 0%, 33%, 50%, and 67% fish meal (FM) protein in a FM-based diet, respectively. The results showed that CGM caused dose-dependent decreases in (1) growth performance, nutrient digestibility, and feed utilization; (2) activities of brush-border membrane enzymes; (3) intestinal antioxidant indices of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase activities, and reduced glutathione level; (4) intestinal immune parameters of acid phosphatase activity, complement 3, complement 4, and IgM concentrations. Dose-dependent increases in the severity of the inflammation, with concomitant alterations on microvilli structure and increasing expression of inflammatory cytokine genes of Il-1β, Il-8, and Tnf-α were observed but without a change in the intracellular junctions and the epithelial permeability established by the plasma diamine oxidase activity and D-lactate level examinations. In conclusion, the present work proved that CGM negatively affected the gut health of turbot by inducing enteritis and by decreasing intestinal immunity and antioxidant capacity, which could be one of the reasons for the reduced utilization of CGM by turbot. Article in Journal/Newspaper Scophthalmus maximus Turbot Directory of Open Access Journals: DOAJ Articles PLOS ONE 14 3 e0213867 |
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Medicine R Science Q Nan Bai Min Gu Mingjie Liu Qian Jia Shihui Pan Zhiyu Zhang Corn gluten meal induces enteritis and decreases intestinal immunity and antioxidant capacity in turbot (Scophthalmus maximus) at high supplementation levels. |
topic_facet |
Medicine R Science Q |
description |
Corn gluten meal (CGM) is an important alternative protein source in aquafeed production. However, in turbot (Scophthalmus maximus), CGM could not be effectively utilized because of its low digestibility, the reason for which is still unclear. The purpose of the present study was to investigate and elucidate the cause for the poor utilization of CGM by turbot from the view of gut health. An 8-week feeding trial was conducted with turbot individuals (initial body weight 11.4 ± 0.2 g), which were fed with one of four isonitrogenous and isolipidic diets formulated to include 0%, 21.2%, 31.8%, and 42.6% CGM to progressively replace 0%, 33%, 50%, and 67% fish meal (FM) protein in a FM-based diet, respectively. The results showed that CGM caused dose-dependent decreases in (1) growth performance, nutrient digestibility, and feed utilization; (2) activities of brush-border membrane enzymes; (3) intestinal antioxidant indices of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase activities, and reduced glutathione level; (4) intestinal immune parameters of acid phosphatase activity, complement 3, complement 4, and IgM concentrations. Dose-dependent increases in the severity of the inflammation, with concomitant alterations on microvilli structure and increasing expression of inflammatory cytokine genes of Il-1β, Il-8, and Tnf-α were observed but without a change in the intracellular junctions and the epithelial permeability established by the plasma diamine oxidase activity and D-lactate level examinations. In conclusion, the present work proved that CGM negatively affected the gut health of turbot by inducing enteritis and by decreasing intestinal immunity and antioxidant capacity, which could be one of the reasons for the reduced utilization of CGM by turbot. |
format |
Article in Journal/Newspaper |
author |
Nan Bai Min Gu Mingjie Liu Qian Jia Shihui Pan Zhiyu Zhang |
author_facet |
Nan Bai Min Gu Mingjie Liu Qian Jia Shihui Pan Zhiyu Zhang |
author_sort |
Nan Bai |
title |
Corn gluten meal induces enteritis and decreases intestinal immunity and antioxidant capacity in turbot (Scophthalmus maximus) at high supplementation levels. |
title_short |
Corn gluten meal induces enteritis and decreases intestinal immunity and antioxidant capacity in turbot (Scophthalmus maximus) at high supplementation levels. |
title_full |
Corn gluten meal induces enteritis and decreases intestinal immunity and antioxidant capacity in turbot (Scophthalmus maximus) at high supplementation levels. |
title_fullStr |
Corn gluten meal induces enteritis and decreases intestinal immunity and antioxidant capacity in turbot (Scophthalmus maximus) at high supplementation levels. |
title_full_unstemmed |
Corn gluten meal induces enteritis and decreases intestinal immunity and antioxidant capacity in turbot (Scophthalmus maximus) at high supplementation levels. |
title_sort |
corn gluten meal induces enteritis and decreases intestinal immunity and antioxidant capacity in turbot (scophthalmus maximus) at high supplementation levels. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pone.0213867 https://doaj.org/article/e25c3ccf6c1a48408293e05076e062f3 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
PLoS ONE, Vol 14, Iss 3, p e0213867 (2019) |
op_relation |
https://doi.org/10.1371/journal.pone.0213867 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0213867 https://doaj.org/article/e25c3ccf6c1a48408293e05076e062f3 |
op_doi |
https://doi.org/10.1371/journal.pone.0213867 |
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PLOS ONE |
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14 |
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3 |
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e0213867 |
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1766188956919005184 |