Antioxidant and anti–acetylcholinesterase activities of extracts and secondary metabolites from Acacia cyanophylla

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Lotfi Ghribia, Hatem Ghouilaa, Amel Omrib, Malek Besbesb, Hichem Ben Janneta
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2014
• Subjects: Acacia cyanophylla; Isosalipurposide; Naringenin; Quercetin; Antioxidant activity; Anti-acetylcholinesterase activity; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.12980/APJTB.4.2014C1038; https://doaj.org/article/e246fb1b22b44f83adf44552429d15cc

Objective: To investigate the antioxidant potential and anti-acetycholinesterase activity of compounds and extracts from Acacia cyanophylla (A. cyanophylla). Methods: Three polyphenolic compounds were isolated from ethyl acetate extract of A. cyanophylla flowers. They have been identified as isosalipurposide 1, quercetin 2 and naringenin 3. Their structures were elucidated by extensive spectroscopic methods including 1D and 2D NMR experiments as well as ES-MS. The prepared extracts and the isolated compounds 1–3 were tested for their antioxidant activity using 1′-1′-diphenylpicrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) scavenging assays and reducing power. They have been also investigated for inhibitory effect against acetylcholinesterase using the microplate assay. Results: In the DPPH test, the EtOAc extract of flowers exhibited the highest antioxidant effect (67.26 μg/mL). Isosalipurposide 1 showed a significant antiradical power against DPPH (81.9 μg/mL). All extracts showed a dose-dependent acetylcholinesterase inhibition. In terms of the IC50 value, the butanolic extract (16.03 μg/mL) was the most potent sample. Isosalipurposide 1 was found to be active against AChE with an IC50 value of 52.04 μg/mL. Conclusions: The results demonstrated the important antioxidant and anti-acetylcholinesterase activity of pure compounds and extracts from A. cyanophylla.