Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras
BACKGROUND Although first detected in animals, the rare rotavirus strain G10P[14] has been sporadically detected in humans in Slovenia, Thailand, United Kingdom and Australia among other countries. Earlier studies suggest that the strains found in humans resulted from interspecies transmission and r...
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ftdoajarticles:oai:doaj.org/article:e2356345d7204891b1b3b8342b89ba94 2023-05-15T15:16:33+02:00 Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras Osbourne Quaye Sunando Roy Kunchala Rungsrisuriyachai Mathew D Esona Ziqian Xu Ka Ian Tam Dina J Castro Banegas Gloria Rey-Benito Michael D Bowen https://doi.org/10.1590/0074-02760170083 https://doaj.org/article/e2356345d7204891b1b3b8342b89ba94 EN eng Instituto Oswaldo Cruz, Ministério da Saúde http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000100009&lng=en&tlng=en https://doaj.org/toc/1678-8060 1678-8060 doi:10.1590/0074-02760170083 https://doaj.org/article/e2356345d7204891b1b3b8342b89ba94 Memórias do Instituto Oswaldo Cruz., Vol 113, Iss 1, Pp 9-16 rotavirus viral genome viral proteins non-structural proteins reassortant Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 article ftdoajarticles https://doi.org/10.1590/0074-02760170083 2023-01-08T01:28:38Z BACKGROUND Although first detected in animals, the rare rotavirus strain G10P[14] has been sporadically detected in humans in Slovenia, Thailand, United Kingdom and Australia among other countries. Earlier studies suggest that the strains found in humans resulted from interspecies transmission and reassortment between human and bovine rotavirus strains. OBJECTIVES In this study, a G10P[14] rotavirus genotype detected in a human stool sample in Honduras during the 2010-2011 rotavirus season, from an unvaccinated 30-month old boy who reported at the hospital with severe diarrhea and vomiting, was characterised to determine the possible evolutionary origin of the rare strain. METHODS For the sample detected as G10P[14], 10% suspension was prepared and used for RNA extraction and sequence independent amplification. The amplicons were sequenced by next-generation sequencing using the Illumina MiSeq 150 paired end method. The sequence reads were analysed using CLC Genomics Workbench 6.0 and phylogenetic trees were constructed using PhyML version 3.0. FINDINGS The next generation sequencing and phylogenetic analyses of the 11-segmented genome of the G10P[14] strain allowed classification as G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. Six of the genes (VP1, VP2, VP3, VP6, NSP2 and NSP4) were DS-1-like. NSP1 and NSP5 were AU-1-like and NSP3 was T6, which suggests that multiple reassortment events occurred in the evolution of the strain. The phylogenetic analyses and genetic distance calculations showed that the VP7, VP4, VP6, VP1, VP3, NSP1, NSP3 and NSP4 genes clustered predominantly with bovine strains. NSP2 and VP2 genes were most closely related to simian and human strains, respectively, and NSP5 was most closely related to a rhesus strain. MAIN CONCLUSIONS The genetic characterisation of the G10P[14] strain from Honduras suggests that its genome resulted from multiple reassortment events which were possibly mediated through interspecies transmissions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Memórias do Instituto Oswaldo Cruz 113 1 9 16 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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rotavirus viral genome viral proteins non-structural proteins reassortant Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
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rotavirus viral genome viral proteins non-structural proteins reassortant Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 Osbourne Quaye Sunando Roy Kunchala Rungsrisuriyachai Mathew D Esona Ziqian Xu Ka Ian Tam Dina J Castro Banegas Gloria Rey-Benito Michael D Bowen Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras |
topic_facet |
rotavirus viral genome viral proteins non-structural proteins reassortant Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
description |
BACKGROUND Although first detected in animals, the rare rotavirus strain G10P[14] has been sporadically detected in humans in Slovenia, Thailand, United Kingdom and Australia among other countries. Earlier studies suggest that the strains found in humans resulted from interspecies transmission and reassortment between human and bovine rotavirus strains. OBJECTIVES In this study, a G10P[14] rotavirus genotype detected in a human stool sample in Honduras during the 2010-2011 rotavirus season, from an unvaccinated 30-month old boy who reported at the hospital with severe diarrhea and vomiting, was characterised to determine the possible evolutionary origin of the rare strain. METHODS For the sample detected as G10P[14], 10% suspension was prepared and used for RNA extraction and sequence independent amplification. The amplicons were sequenced by next-generation sequencing using the Illumina MiSeq 150 paired end method. The sequence reads were analysed using CLC Genomics Workbench 6.0 and phylogenetic trees were constructed using PhyML version 3.0. FINDINGS The next generation sequencing and phylogenetic analyses of the 11-segmented genome of the G10P[14] strain allowed classification as G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. Six of the genes (VP1, VP2, VP3, VP6, NSP2 and NSP4) were DS-1-like. NSP1 and NSP5 were AU-1-like and NSP3 was T6, which suggests that multiple reassortment events occurred in the evolution of the strain. The phylogenetic analyses and genetic distance calculations showed that the VP7, VP4, VP6, VP1, VP3, NSP1, NSP3 and NSP4 genes clustered predominantly with bovine strains. NSP2 and VP2 genes were most closely related to simian and human strains, respectively, and NSP5 was most closely related to a rhesus strain. MAIN CONCLUSIONS The genetic characterisation of the G10P[14] strain from Honduras suggests that its genome resulted from multiple reassortment events which were possibly mediated through interspecies transmissions. |
format |
Article in Journal/Newspaper |
author |
Osbourne Quaye Sunando Roy Kunchala Rungsrisuriyachai Mathew D Esona Ziqian Xu Ka Ian Tam Dina J Castro Banegas Gloria Rey-Benito Michael D Bowen |
author_facet |
Osbourne Quaye Sunando Roy Kunchala Rungsrisuriyachai Mathew D Esona Ziqian Xu Ka Ian Tam Dina J Castro Banegas Gloria Rey-Benito Michael D Bowen |
author_sort |
Osbourne Quaye |
title |
Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras |
title_short |
Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras |
title_full |
Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras |
title_fullStr |
Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras |
title_full_unstemmed |
Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras |
title_sort |
characterisation of a rare, reassortant human g10p[14] rotavirus strain detected in honduras |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
url |
https://doi.org/10.1590/0074-02760170083 https://doaj.org/article/e2356345d7204891b1b3b8342b89ba94 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Memórias do Instituto Oswaldo Cruz., Vol 113, Iss 1, Pp 9-16 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000100009&lng=en&tlng=en https://doaj.org/toc/1678-8060 1678-8060 doi:10.1590/0074-02760170083 https://doaj.org/article/e2356345d7204891b1b3b8342b89ba94 |
op_doi |
https://doi.org/10.1590/0074-02760170083 |
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Memórias do Instituto Oswaldo Cruz |
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113 |
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