The Spleen as a Target to Characterize Immunomodulatory Effects of Down-Stream Processed Cyberlindnera jadinii Yeasts in Atlantic Salmon Exposed to a Dietary Soybean Meal Challenge
Aquaculture feeds have changed dramatically from being largely based on fishmeal (FM) towards increased use of plant protein sources, which could impact the fish’s immune response. In order to characterize immunomodulatory properties of novel functional ingredients, this study used four diets, one b...
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ftdoajarticles:oai:doaj.org/article:e20f6b6b42104f6ea0405924ad7dea9a 2023-05-15T15:30:53+02:00 The Spleen as a Target to Characterize Immunomodulatory Effects of Down-Stream Processed Cyberlindnera jadinii Yeasts in Atlantic Salmon Exposed to a Dietary Soybean Meal Challenge Byron Morales-Lange Jeleel Opeyemi Agboola Jon Øvrum Hansen Leidy Lagos Ove Øyås Luis Mercado Liv Torunn Mydland Margareth Øverland 2021-08-01T00:00:00Z https://doi.org/10.3389/fimmu.2021.708747 https://doaj.org/article/e20f6b6b42104f6ea0405924ad7dea9a EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fimmu.2021.708747/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2021.708747 https://doaj.org/article/e20f6b6b42104f6ea0405924ad7dea9a Frontiers in Immunology, Vol 12 (2021) Salmo salar transcriptomics ELISA secondary lymphoid organ inactivated yeast autolysed yeast Immunologic diseases. Allergy RC581-607 article 2021 ftdoajarticles https://doi.org/10.3389/fimmu.2021.708747 2022-12-31T13:10:54Z Aquaculture feeds have changed dramatically from being largely based on fishmeal (FM) towards increased use of plant protein sources, which could impact the fish’s immune response. In order to characterize immunomodulatory properties of novel functional ingredients, this study used four diets, one based on FM, a challenging diet with 40% soybean meal (SBM), and two diets containing 40% SBM with 5% of Cyberlindnera jadinii yeast exposed to different down-stream processing conditions: heat-inactivated (ICJ) or autolysation (ACJ). The immunomodulatory effects of the diets were analyzed in the spleen of Atlantic salmon after 37 days of feeding, using a transcriptomic evaluation by RNA sequencing (RNA-seq) and the detection of specific immunological markers at the protein level through indirect Enzyme-linked Immunosorbent Assay (indirect ELISA). The results showed that SBM (compared to FM) induced a down-regulation of pathways related to ion binding and transport, along with an increase at the protein level of pro-inflammatory cytokines such as tumor necrosis factor alpha (TNFα) and interferon gamma (IFNγ). On the other hand, while ICJ (compared to FM-group) maintain the inflammatory response associated with SBM, with higher levels of TNFα and IFNγ, and with an upregulation of creatine kinase activity and phosphagen metabolic process, the inclusion of ACJ was able to modulate the response of Atlantic salmon compared to fish fed the SBM-diet by the activation of biological pathways related to endocytosis, Pattern recognition receptor (PPRs)-signal transduction and transporter activity. In addition, ACJ was also able to control the pro-inflammatory profile of SBM, increasing Interleukin 10 (IL-10) levels and decreasing TNFα production, triggering an immune response similar to that of fish fed an FM-based diet. Finally, we suggest that the spleen is a good candidate to characterize the immunomodulatory effects of functional ingredients in Atlantic salmon. Moreover, the inclusion of ACJ in fish diets, with the ability to ... Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Frontiers in Immunology 12 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Salmo salar transcriptomics ELISA secondary lymphoid organ inactivated yeast autolysed yeast Immunologic diseases. Allergy RC581-607 |
spellingShingle |
Salmo salar transcriptomics ELISA secondary lymphoid organ inactivated yeast autolysed yeast Immunologic diseases. Allergy RC581-607 Byron Morales-Lange Jeleel Opeyemi Agboola Jon Øvrum Hansen Leidy Lagos Ove Øyås Luis Mercado Liv Torunn Mydland Margareth Øverland The Spleen as a Target to Characterize Immunomodulatory Effects of Down-Stream Processed Cyberlindnera jadinii Yeasts in Atlantic Salmon Exposed to a Dietary Soybean Meal Challenge |
topic_facet |
Salmo salar transcriptomics ELISA secondary lymphoid organ inactivated yeast autolysed yeast Immunologic diseases. Allergy RC581-607 |
description |
Aquaculture feeds have changed dramatically from being largely based on fishmeal (FM) towards increased use of plant protein sources, which could impact the fish’s immune response. In order to characterize immunomodulatory properties of novel functional ingredients, this study used four diets, one based on FM, a challenging diet with 40% soybean meal (SBM), and two diets containing 40% SBM with 5% of Cyberlindnera jadinii yeast exposed to different down-stream processing conditions: heat-inactivated (ICJ) or autolysation (ACJ). The immunomodulatory effects of the diets were analyzed in the spleen of Atlantic salmon after 37 days of feeding, using a transcriptomic evaluation by RNA sequencing (RNA-seq) and the detection of specific immunological markers at the protein level through indirect Enzyme-linked Immunosorbent Assay (indirect ELISA). The results showed that SBM (compared to FM) induced a down-regulation of pathways related to ion binding and transport, along with an increase at the protein level of pro-inflammatory cytokines such as tumor necrosis factor alpha (TNFα) and interferon gamma (IFNγ). On the other hand, while ICJ (compared to FM-group) maintain the inflammatory response associated with SBM, with higher levels of TNFα and IFNγ, and with an upregulation of creatine kinase activity and phosphagen metabolic process, the inclusion of ACJ was able to modulate the response of Atlantic salmon compared to fish fed the SBM-diet by the activation of biological pathways related to endocytosis, Pattern recognition receptor (PPRs)-signal transduction and transporter activity. In addition, ACJ was also able to control the pro-inflammatory profile of SBM, increasing Interleukin 10 (IL-10) levels and decreasing TNFα production, triggering an immune response similar to that of fish fed an FM-based diet. Finally, we suggest that the spleen is a good candidate to characterize the immunomodulatory effects of functional ingredients in Atlantic salmon. Moreover, the inclusion of ACJ in fish diets, with the ability to ... |
format |
Article in Journal/Newspaper |
author |
Byron Morales-Lange Jeleel Opeyemi Agboola Jon Øvrum Hansen Leidy Lagos Ove Øyås Luis Mercado Liv Torunn Mydland Margareth Øverland |
author_facet |
Byron Morales-Lange Jeleel Opeyemi Agboola Jon Øvrum Hansen Leidy Lagos Ove Øyås Luis Mercado Liv Torunn Mydland Margareth Øverland |
author_sort |
Byron Morales-Lange |
title |
The Spleen as a Target to Characterize Immunomodulatory Effects of Down-Stream Processed Cyberlindnera jadinii Yeasts in Atlantic Salmon Exposed to a Dietary Soybean Meal Challenge |
title_short |
The Spleen as a Target to Characterize Immunomodulatory Effects of Down-Stream Processed Cyberlindnera jadinii Yeasts in Atlantic Salmon Exposed to a Dietary Soybean Meal Challenge |
title_full |
The Spleen as a Target to Characterize Immunomodulatory Effects of Down-Stream Processed Cyberlindnera jadinii Yeasts in Atlantic Salmon Exposed to a Dietary Soybean Meal Challenge |
title_fullStr |
The Spleen as a Target to Characterize Immunomodulatory Effects of Down-Stream Processed Cyberlindnera jadinii Yeasts in Atlantic Salmon Exposed to a Dietary Soybean Meal Challenge |
title_full_unstemmed |
The Spleen as a Target to Characterize Immunomodulatory Effects of Down-Stream Processed Cyberlindnera jadinii Yeasts in Atlantic Salmon Exposed to a Dietary Soybean Meal Challenge |
title_sort |
spleen as a target to characterize immunomodulatory effects of down-stream processed cyberlindnera jadinii yeasts in atlantic salmon exposed to a dietary soybean meal challenge |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2021.708747 https://doaj.org/article/e20f6b6b42104f6ea0405924ad7dea9a |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Frontiers in Immunology, Vol 12 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fimmu.2021.708747/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2021.708747 https://doaj.org/article/e20f6b6b42104f6ea0405924ad7dea9a |
op_doi |
https://doi.org/10.3389/fimmu.2021.708747 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
_version_ |
1766361361755930624 |