Activation energy of extracellular enzymes in soils from different biomes.
Enzyme dynamics are being incorporated into soil carbon cycling models and accurate representation of enzyme kinetics is an important step in predicting belowground nutrient dynamics. A scarce number of studies have measured activation energy (Ea) in soils and fewer studies have measured Ea in arcti...
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ftdoajarticles:oai:doaj.org/article:e196c0e5611a48f4b6a56f4c8324ce21 2023-05-15T14:59:48+02:00 Activation energy of extracellular enzymes in soils from different biomes. J Megan Steinweg Sindhu Jagadamma Joshua Frerichs Melanie A Mayes 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0059943 https://doaj.org/article/e196c0e5611a48f4b6a56f4c8324ce21 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3607567?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0059943 https://doaj.org/article/e196c0e5611a48f4b6a56f4c8324ce21 PLoS ONE, Vol 8, Iss 3, p e59943 (2013) Medicine R Science Q article 2013 ftdoajarticles https://doi.org/10.1371/journal.pone.0059943 2022-12-31T12:03:46Z Enzyme dynamics are being incorporated into soil carbon cycling models and accurate representation of enzyme kinetics is an important step in predicting belowground nutrient dynamics. A scarce number of studies have measured activation energy (Ea) in soils and fewer studies have measured Ea in arctic and tropical soils, or in subsurface soils. We determined the Ea for four typical lignocellulose degrading enzymes in the A and B horizons of seven soils covering six different soil orders. We also elucidated which soil properties predicted any measurable differences in Ea. β-glucosidase, cellobiohydrolase, phenol oxidase and peroxidase activities were measured at five temperatures, 4, 21, 30, 40, and 60°C. Ea was calculated using the Arrhenius equation. β-glucosidase and cellobiohydrolase Ea values for both A and B horizons in this study were similar to previously reported values, however we could not make a direct comparison for B horizon soils because of the lack of data. There was no consistent relationship between hydrolase enzyme Ea and the environmental variables we measured. Phenol oxidase was the only enzyme that had a consistent positive relationship between Ea and pH in both horizons. The Ea in the arctic and subarctic zones for peroxidase was lower than the hydrolases and phenol oxidase values, indicating peroxidase may be a rate limited enzyme in environments under warming conditions. By including these six soil types we have increased the number of soil oxidative enzyme Ea values reported in the literature by 50%. This study is a step towards better quantifying enzyme kinetics in different climate zones. Article in Journal/Newspaper Arctic Subarctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS ONE 8 3 e59943 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Medicine R Science Q |
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Medicine R Science Q J Megan Steinweg Sindhu Jagadamma Joshua Frerichs Melanie A Mayes Activation energy of extracellular enzymes in soils from different biomes. |
topic_facet |
Medicine R Science Q |
description |
Enzyme dynamics are being incorporated into soil carbon cycling models and accurate representation of enzyme kinetics is an important step in predicting belowground nutrient dynamics. A scarce number of studies have measured activation energy (Ea) in soils and fewer studies have measured Ea in arctic and tropical soils, or in subsurface soils. We determined the Ea for four typical lignocellulose degrading enzymes in the A and B horizons of seven soils covering six different soil orders. We also elucidated which soil properties predicted any measurable differences in Ea. β-glucosidase, cellobiohydrolase, phenol oxidase and peroxidase activities were measured at five temperatures, 4, 21, 30, 40, and 60°C. Ea was calculated using the Arrhenius equation. β-glucosidase and cellobiohydrolase Ea values for both A and B horizons in this study were similar to previously reported values, however we could not make a direct comparison for B horizon soils because of the lack of data. There was no consistent relationship between hydrolase enzyme Ea and the environmental variables we measured. Phenol oxidase was the only enzyme that had a consistent positive relationship between Ea and pH in both horizons. The Ea in the arctic and subarctic zones for peroxidase was lower than the hydrolases and phenol oxidase values, indicating peroxidase may be a rate limited enzyme in environments under warming conditions. By including these six soil types we have increased the number of soil oxidative enzyme Ea values reported in the literature by 50%. This study is a step towards better quantifying enzyme kinetics in different climate zones. |
format |
Article in Journal/Newspaper |
author |
J Megan Steinweg Sindhu Jagadamma Joshua Frerichs Melanie A Mayes |
author_facet |
J Megan Steinweg Sindhu Jagadamma Joshua Frerichs Melanie A Mayes |
author_sort |
J Megan Steinweg |
title |
Activation energy of extracellular enzymes in soils from different biomes. |
title_short |
Activation energy of extracellular enzymes in soils from different biomes. |
title_full |
Activation energy of extracellular enzymes in soils from different biomes. |
title_fullStr |
Activation energy of extracellular enzymes in soils from different biomes. |
title_full_unstemmed |
Activation energy of extracellular enzymes in soils from different biomes. |
title_sort |
activation energy of extracellular enzymes in soils from different biomes. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pone.0059943 https://doaj.org/article/e196c0e5611a48f4b6a56f4c8324ce21 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Subarctic |
genre_facet |
Arctic Subarctic |
op_source |
PLoS ONE, Vol 8, Iss 3, p e59943 (2013) |
op_relation |
http://europepmc.org/articles/PMC3607567?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0059943 https://doaj.org/article/e196c0e5611a48f4b6a56f4c8324ce21 |
op_doi |
https://doi.org/10.1371/journal.pone.0059943 |
container_title |
PLoS ONE |
container_volume |
8 |
container_issue |
3 |
container_start_page |
e59943 |
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1766331927049011200 |