Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution
Abstract Background Fish skin mucosal surfaces (SMS) are quite similar in composition and function to some mammalian MS and, in consequence, could constitute an adequate niche for the evolution of mucosal aquatic pathogens in natural environments. We aimed to test this hypothesis by searching for me...
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ftdoajarticles:oai:doaj.org/article:e17a2e191a3d47ffb55f0841a4572c0c 2023-05-15T13:28:11+02:00 Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution Miguel Carda-Diéguez Rohit Ghai Francisco Rodríguez-Valera Carmen Amaro 2017-12-01T00:00:00Z https://doi.org/10.1186/s40168-017-0376-1 https://doaj.org/article/e17a2e191a3d47ffb55f0841a4572c0c EN eng BMC http://link.springer.com/article/10.1186/s40168-017-0376-1 https://doaj.org/toc/2049-2618 doi:10.1186/s40168-017-0376-1 2049-2618 https://doaj.org/article/e17a2e191a3d47ffb55f0841a4572c0c Microbiome, Vol 5, Iss 1, Pp 1-15 (2017) Vibrio Skin mucus Microbiome Metagenomics Attached microbiota Microbial ecology QR100-130 article 2017 ftdoajarticles https://doi.org/10.1186/s40168-017-0376-1 2022-12-31T14:14:15Z Abstract Background Fish skin mucosal surfaces (SMS) are quite similar in composition and function to some mammalian MS and, in consequence, could constitute an adequate niche for the evolution of mucosal aquatic pathogens in natural environments. We aimed to test this hypothesis by searching for metagenomic and genomic evidences in the SMS-microbiome of a model fish species (Anguilla Anguilla or eel), from different ecosystems (four natural environments of different water salinity and one eel farm) as well as the water microbiome (W-microbiome) surrounding the host. Results Remarkably, potentially pathogenic Vibrio monopolized wild eel SMS-microbiome from natural ecosystems, Vibrio anguillarum/Vibrio vulnificus and Vibrio cholerae/Vibrio metoecus being the most abundant ones in SMS from estuary and lake, respectively. Functions encoded in the SMS-microbiome differed significantly from those in the W-microbiome and allowed us to predict that successful mucus colonizers should have specific genes for (i) attachment (mainly by forming biofilms), (ii) bacterial competence and communication, and (iii) resistance to mucosal innate immunity, predators (amoeba), and heavy metals/drugs. In addition, we found several mobile genetic elements (mainly integrative conjugative elements) as well as a series of evidences suggesting that bacteria exchange DNA in SMS. Further, we isolated and sequenced a V. metoecus strain from SMS. This isolate shares pathogenicity islands with V. cholerae O1 from intestinal infections that are absent in the rest of sequenced V. metoecus strains, all of them from water and extra-intestinal infections. Conclusions We have obtained metagenomic and genomic evidence in favor of the hypothesis on the role of fish mucosal surfaces as a specialized habitat selecting microbes capable of colonizing and persisting on other comparable mucosal surfaces, e.g., the human intestine. Article in Journal/Newspaper Anguilla anguilla Directory of Open Access Journals: DOAJ Articles Microbiome 5 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Vibrio Skin mucus Microbiome Metagenomics Attached microbiota Microbial ecology QR100-130 |
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Vibrio Skin mucus Microbiome Metagenomics Attached microbiota Microbial ecology QR100-130 Miguel Carda-Diéguez Rohit Ghai Francisco Rodríguez-Valera Carmen Amaro Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution |
topic_facet |
Vibrio Skin mucus Microbiome Metagenomics Attached microbiota Microbial ecology QR100-130 |
description |
Abstract Background Fish skin mucosal surfaces (SMS) are quite similar in composition and function to some mammalian MS and, in consequence, could constitute an adequate niche for the evolution of mucosal aquatic pathogens in natural environments. We aimed to test this hypothesis by searching for metagenomic and genomic evidences in the SMS-microbiome of a model fish species (Anguilla Anguilla or eel), from different ecosystems (four natural environments of different water salinity and one eel farm) as well as the water microbiome (W-microbiome) surrounding the host. Results Remarkably, potentially pathogenic Vibrio monopolized wild eel SMS-microbiome from natural ecosystems, Vibrio anguillarum/Vibrio vulnificus and Vibrio cholerae/Vibrio metoecus being the most abundant ones in SMS from estuary and lake, respectively. Functions encoded in the SMS-microbiome differed significantly from those in the W-microbiome and allowed us to predict that successful mucus colonizers should have specific genes for (i) attachment (mainly by forming biofilms), (ii) bacterial competence and communication, and (iii) resistance to mucosal innate immunity, predators (amoeba), and heavy metals/drugs. In addition, we found several mobile genetic elements (mainly integrative conjugative elements) as well as a series of evidences suggesting that bacteria exchange DNA in SMS. Further, we isolated and sequenced a V. metoecus strain from SMS. This isolate shares pathogenicity islands with V. cholerae O1 from intestinal infections that are absent in the rest of sequenced V. metoecus strains, all of them from water and extra-intestinal infections. Conclusions We have obtained metagenomic and genomic evidence in favor of the hypothesis on the role of fish mucosal surfaces as a specialized habitat selecting microbes capable of colonizing and persisting on other comparable mucosal surfaces, e.g., the human intestine. |
format |
Article in Journal/Newspaper |
author |
Miguel Carda-Diéguez Rohit Ghai Francisco Rodríguez-Valera Carmen Amaro |
author_facet |
Miguel Carda-Diéguez Rohit Ghai Francisco Rodríguez-Valera Carmen Amaro |
author_sort |
Miguel Carda-Diéguez |
title |
Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution |
title_short |
Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution |
title_full |
Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution |
title_fullStr |
Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution |
title_full_unstemmed |
Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution |
title_sort |
wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s40168-017-0376-1 https://doaj.org/article/e17a2e191a3d47ffb55f0841a4572c0c |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Microbiome, Vol 5, Iss 1, Pp 1-15 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s40168-017-0376-1 https://doaj.org/toc/2049-2618 doi:10.1186/s40168-017-0376-1 2049-2618 https://doaj.org/article/e17a2e191a3d47ffb55f0841a4572c0c |
op_doi |
https://doi.org/10.1186/s40168-017-0376-1 |
container_title |
Microbiome |
container_volume |
5 |
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1 |
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1766402672688103424 |