Molecular Detection and Characterization of the First Cowpox Virus Isolate Derived from a Bank Vole
Cowpox virus (CPXV) is a zoonotic orthopoxvirus (OPV) that infects a wide range of mammals. CPXV-specific DNA and antibodies were detected in different vole species, such as common voles ( Microtus arvalis ) and bank voles ( Myodes glareolus ). Therefore, voles are the putative main reservoir host o...
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ftdoajarticles:oai:doaj.org/article:e1644d38f3254195a575413e4d1e2680 2023-05-15T17:12:35+02:00 Molecular Detection and Characterization of the First Cowpox Virus Isolate Derived from a Bank Vole Kathrin Jeske Saskia Weber Florian Pfaff Christian Imholt Jens Jacob Martin Beer Rainer G. Ulrich Donata Hoffmann 2019-11-01T00:00:00Z https://doi.org/10.3390/v11111075 https://doaj.org/article/e1644d38f3254195a575413e4d1e2680 EN eng MDPI AG https://www.mdpi.com/1999-4915/11/11/1075 https://doaj.org/toc/1999-4915 1999-4915 doi:10.3390/v11111075 https://doaj.org/article/e1644d38f3254195a575413e4d1e2680 Viruses, Vol 11, Iss 11, p 1075 (2019) bank vole myodes glareolus cowpox virus orthopoxvirus germany Microbiology QR1-502 article 2019 ftdoajarticles https://doi.org/10.3390/v11111075 2022-12-31T01:21:58Z Cowpox virus (CPXV) is a zoonotic orthopoxvirus (OPV) that infects a wide range of mammals. CPXV-specific DNA and antibodies were detected in different vole species, such as common voles ( Microtus arvalis ) and bank voles ( Myodes glareolus ). Therefore, voles are the putative main reservoir host of CPXV. However, CPXV was up to now only isolated from common voles. Here we report the detection and isolation of a bank vole-derived CPXV strain (GerMygEK 938/17) resulting from a large-scale screening of bank voles collected in Thuringia, Germany, during 2017 and 2018. Phylogenetic analysis using the complete viral genome sequence indicated a high similarity of the novel strain to CPXV clade 3 and to OPV “Abatino” but also to Ectromelia virus (ECTV) strains. Phenotypic characterization of CPXV GerMygEK 938/17 using inoculation of embryonated chicken eggs displayed hemorrhagic pock lesions on the chorioallantoic membrane that are typical for CPXV but not for ECTV. CPXV GerMygEK 938/17 replicated in vole-derived kidney cell lines but at lower level than on Vero76 cell line. In conclusion, the first bank vole-derived CPXV isolate provides new insights into the genetic variability of CPXV in the putative reservoir host and is a valuable tool for further studies about CPXV-host interaction and molecular evolution of OPV. Article in Journal/Newspaper Microtus arvalis Directory of Open Access Journals: DOAJ Articles Viruses 11 11 1075 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
bank vole myodes glareolus cowpox virus orthopoxvirus germany Microbiology QR1-502 |
spellingShingle |
bank vole myodes glareolus cowpox virus orthopoxvirus germany Microbiology QR1-502 Kathrin Jeske Saskia Weber Florian Pfaff Christian Imholt Jens Jacob Martin Beer Rainer G. Ulrich Donata Hoffmann Molecular Detection and Characterization of the First Cowpox Virus Isolate Derived from a Bank Vole |
topic_facet |
bank vole myodes glareolus cowpox virus orthopoxvirus germany Microbiology QR1-502 |
description |
Cowpox virus (CPXV) is a zoonotic orthopoxvirus (OPV) that infects a wide range of mammals. CPXV-specific DNA and antibodies were detected in different vole species, such as common voles ( Microtus arvalis ) and bank voles ( Myodes glareolus ). Therefore, voles are the putative main reservoir host of CPXV. However, CPXV was up to now only isolated from common voles. Here we report the detection and isolation of a bank vole-derived CPXV strain (GerMygEK 938/17) resulting from a large-scale screening of bank voles collected in Thuringia, Germany, during 2017 and 2018. Phylogenetic analysis using the complete viral genome sequence indicated a high similarity of the novel strain to CPXV clade 3 and to OPV “Abatino” but also to Ectromelia virus (ECTV) strains. Phenotypic characterization of CPXV GerMygEK 938/17 using inoculation of embryonated chicken eggs displayed hemorrhagic pock lesions on the chorioallantoic membrane that are typical for CPXV but not for ECTV. CPXV GerMygEK 938/17 replicated in vole-derived kidney cell lines but at lower level than on Vero76 cell line. In conclusion, the first bank vole-derived CPXV isolate provides new insights into the genetic variability of CPXV in the putative reservoir host and is a valuable tool for further studies about CPXV-host interaction and molecular evolution of OPV. |
format |
Article in Journal/Newspaper |
author |
Kathrin Jeske Saskia Weber Florian Pfaff Christian Imholt Jens Jacob Martin Beer Rainer G. Ulrich Donata Hoffmann |
author_facet |
Kathrin Jeske Saskia Weber Florian Pfaff Christian Imholt Jens Jacob Martin Beer Rainer G. Ulrich Donata Hoffmann |
author_sort |
Kathrin Jeske |
title |
Molecular Detection and Characterization of the First Cowpox Virus Isolate Derived from a Bank Vole |
title_short |
Molecular Detection and Characterization of the First Cowpox Virus Isolate Derived from a Bank Vole |
title_full |
Molecular Detection and Characterization of the First Cowpox Virus Isolate Derived from a Bank Vole |
title_fullStr |
Molecular Detection and Characterization of the First Cowpox Virus Isolate Derived from a Bank Vole |
title_full_unstemmed |
Molecular Detection and Characterization of the First Cowpox Virus Isolate Derived from a Bank Vole |
title_sort |
molecular detection and characterization of the first cowpox virus isolate derived from a bank vole |
publisher |
MDPI AG |
publishDate |
2019 |
url |
https://doi.org/10.3390/v11111075 https://doaj.org/article/e1644d38f3254195a575413e4d1e2680 |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Viruses, Vol 11, Iss 11, p 1075 (2019) |
op_relation |
https://www.mdpi.com/1999-4915/11/11/1075 https://doaj.org/toc/1999-4915 1999-4915 doi:10.3390/v11111075 https://doaj.org/article/e1644d38f3254195a575413e4d1e2680 |
op_doi |
https://doi.org/10.3390/v11111075 |
container_title |
Viruses |
container_volume |
11 |
container_issue |
11 |
container_start_page |
1075 |
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1766069378754805760 |