An improved method for the in vitro differentiation of Plasmodium falciparum gametocytes into ookinetes
Abstract Background Ookinete is the form of the malaria parasite that invades the mosquito midgut epithelium to initiate sporogony. Differentiation of ingested gametocytes into ookinetes in the mosquito midgut lumen and subsequent interaction with the lumenal surface of the midgut epithelium in prep...
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ftdoajarticles:oai:doaj.org/article:e15eab80d35e4e8bafb8c1cbf4a49f03 2023-05-15T15:16:04+02:00 An improved method for the in vitro differentiation of Plasmodium falciparum gametocytes into ookinetes Jacobs-Lorena Marcelo Ikadai Hiromi Dinglasan Rhoel R Ghosh Anil K 2010-07-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-194 https://doaj.org/article/e15eab80d35e4e8bafb8c1cbf4a49f03 EN eng BMC http://www.malariajournal.com/content/9/1/194 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-194 1475-2875 https://doaj.org/article/e15eab80d35e4e8bafb8c1cbf4a49f03 Malaria Journal, Vol 9, Iss 1, p 194 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-194 2022-12-31T09:13:53Z Abstract Background Ookinete is the form of the malaria parasite that invades the mosquito midgut epithelium to initiate sporogony. Differentiation of ingested gametocytes into ookinetes in the mosquito midgut lumen and subsequent interaction with the lumenal surface of the midgut epithelium in preparation for invasion is a complex and multi-stepped process. To facilitate the study of these events in detail it is necessary to produce sufficient numbers of pure, fully mature and functional ookinetes. However, production of even a small number of Plasmodium falciparum ookinetes in vitro has proven to be a daunting task. Consequently, over the past four decades our collective understanding of the biology of this parasite form remains sorely deficient. This article reports on investigations of five different ookinete media, in an effort to improve the in vitro transformation efficiency of P. falciparum gametocytes into mature ookinetes and their infectivity of the mosquito midgut. Methods Five different ookinete media were evaluated for their ability to support the differentiation of gametocytes into gametes and further into mature stage V ookinetes. Moreover, infectivity of the in vitro -transformed ookinetes was evaluated by feeding them to vector mosquitoes and measuring their ability to traverse the midgut and form oocysts. Results One of the five media (medium E) was clearly superior in that the cultured ookinetes produced the largest number of oocysts when fed to mosquitoes. Key components were additions of human serum, human red blood cell lysate and mosquito pupal extract, resulting in the production of larger numbers of ookinetes able to develop into oocysts when fed to mosquitoes. Conclusion This simple and practical improvement over the prevailing methodology will facilitate the investigation of how this important human malaria parasite initiates its development in the mosquito and will contribute to the understanding of its transmission biology. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 9 1 194 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Jacobs-Lorena Marcelo Ikadai Hiromi Dinglasan Rhoel R Ghosh Anil K An improved method for the in vitro differentiation of Plasmodium falciparum gametocytes into ookinetes |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Ookinete is the form of the malaria parasite that invades the mosquito midgut epithelium to initiate sporogony. Differentiation of ingested gametocytes into ookinetes in the mosquito midgut lumen and subsequent interaction with the lumenal surface of the midgut epithelium in preparation for invasion is a complex and multi-stepped process. To facilitate the study of these events in detail it is necessary to produce sufficient numbers of pure, fully mature and functional ookinetes. However, production of even a small number of Plasmodium falciparum ookinetes in vitro has proven to be a daunting task. Consequently, over the past four decades our collective understanding of the biology of this parasite form remains sorely deficient. This article reports on investigations of five different ookinete media, in an effort to improve the in vitro transformation efficiency of P. falciparum gametocytes into mature ookinetes and their infectivity of the mosquito midgut. Methods Five different ookinete media were evaluated for their ability to support the differentiation of gametocytes into gametes and further into mature stage V ookinetes. Moreover, infectivity of the in vitro -transformed ookinetes was evaluated by feeding them to vector mosquitoes and measuring their ability to traverse the midgut and form oocysts. Results One of the five media (medium E) was clearly superior in that the cultured ookinetes produced the largest number of oocysts when fed to mosquitoes. Key components were additions of human serum, human red blood cell lysate and mosquito pupal extract, resulting in the production of larger numbers of ookinetes able to develop into oocysts when fed to mosquitoes. Conclusion This simple and practical improvement over the prevailing methodology will facilitate the investigation of how this important human malaria parasite initiates its development in the mosquito and will contribute to the understanding of its transmission biology. |
format |
Article in Journal/Newspaper |
author |
Jacobs-Lorena Marcelo Ikadai Hiromi Dinglasan Rhoel R Ghosh Anil K |
author_facet |
Jacobs-Lorena Marcelo Ikadai Hiromi Dinglasan Rhoel R Ghosh Anil K |
author_sort |
Jacobs-Lorena Marcelo |
title |
An improved method for the in vitro differentiation of Plasmodium falciparum gametocytes into ookinetes |
title_short |
An improved method for the in vitro differentiation of Plasmodium falciparum gametocytes into ookinetes |
title_full |
An improved method for the in vitro differentiation of Plasmodium falciparum gametocytes into ookinetes |
title_fullStr |
An improved method for the in vitro differentiation of Plasmodium falciparum gametocytes into ookinetes |
title_full_unstemmed |
An improved method for the in vitro differentiation of Plasmodium falciparum gametocytes into ookinetes |
title_sort |
improved method for the in vitro differentiation of plasmodium falciparum gametocytes into ookinetes |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-194 https://doaj.org/article/e15eab80d35e4e8bafb8c1cbf4a49f03 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 194 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/194 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-194 1475-2875 https://doaj.org/article/e15eab80d35e4e8bafb8c1cbf4a49f03 |
op_doi |
https://doi.org/10.1186/1475-2875-9-194 |
container_title |
Malaria Journal |
container_volume |
9 |
container_issue |
1 |
container_start_page |
194 |
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1766346372789829632 |