Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
Introduction Herpes simplex virus (HSV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fluid samples has allowed the detection of viral DNA with high s...
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ftdoajarticles:oai:doaj.org/article:e14dfe78456f4f9f9c6d8206506730a9 2023-05-15T15:01:26+02:00 Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus Diogenes Rodrigues Fernanda de-Paris Rodrigo Minuto Paiva 2013-09-01T00:00:00Z https://doi.org/10.1590/0037-8682-1520-2013 https://doaj.org/article/e14dfe78456f4f9f9c6d8206506730a9 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000500625&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-1520-2013 https://doaj.org/article/e14dfe78456f4f9f9c6d8206506730a9 Revista da Sociedade Brasileira de Medicina Tropical, Vol 46, Iss 5, Pp 625-628 (2013) Herpes simplex virus Varicella zoster virus Polymerase chain reaction Arctic medicine. Tropical medicine RC955-962 article 2013 ftdoajarticles https://doi.org/10.1590/0037-8682-1520-2013 2022-12-30T23:06:44Z Introduction Herpes simplex virus (HSV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fluid samples has allowed the detection of viral DNA with high sensitivity and specificity. Methods Serial dilutions of quantified commercial controls of each virus were subjected to an in-house nested-PCR technique. Results The minimum detection limits for HSV and VZV were 5 and 10 copies/µL, respectively. Conclusions The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 46 5 625 628 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Herpes simplex virus Varicella zoster virus Polymerase chain reaction Arctic medicine. Tropical medicine RC955-962 |
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Herpes simplex virus Varicella zoster virus Polymerase chain reaction Arctic medicine. Tropical medicine RC955-962 Diogenes Rodrigues Fernanda de-Paris Rodrigo Minuto Paiva Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus |
topic_facet |
Herpes simplex virus Varicella zoster virus Polymerase chain reaction Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction Herpes simplex virus (HSV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fluid samples has allowed the detection of viral DNA with high sensitivity and specificity. Methods Serial dilutions of quantified commercial controls of each virus were subjected to an in-house nested-PCR technique. Results The minimum detection limits for HSV and VZV were 5 and 10 copies/µL, respectively. Conclusions The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies. |
format |
Article in Journal/Newspaper |
author |
Diogenes Rodrigues Fernanda de-Paris Rodrigo Minuto Paiva |
author_facet |
Diogenes Rodrigues Fernanda de-Paris Rodrigo Minuto Paiva |
author_sort |
Diogenes Rodrigues |
title |
Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus |
title_short |
Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus |
title_full |
Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus |
title_fullStr |
Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus |
title_full_unstemmed |
Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus |
title_sort |
minimum detection limit of an in-house nested-pcr assay for herpes simplex virus and varicella zoster virus |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2013 |
url |
https://doi.org/10.1590/0037-8682-1520-2013 https://doaj.org/article/e14dfe78456f4f9f9c6d8206506730a9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 46, Iss 5, Pp 625-628 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000500625&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-1520-2013 https://doaj.org/article/e14dfe78456f4f9f9c6d8206506730a9 |
op_doi |
https://doi.org/10.1590/0037-8682-1520-2013 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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46 |
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625 |
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628 |
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