Physicochemical and Biochemical Properties of Trypsin-like Enzyme from Two Sturgeon Species

This work aimed to determine the physicochemical and biochemical properties of trypsin from beluga Huso huso and sevruga Acipenser stellatus, two highly valuable sturgeon species. According to the results obtained from the methods of casein-zymogram and inhibitory activity staining, the molecular we...

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Bibliographic Details
Published in:Animals
Main Authors: Abbas Zamani, Maryam Khajavi, Abdolmohammad Abedian Kenari, Masoumeh Haghbin Nazarpak, Atefeh Solouk, Mina Esmaeili, Enric Gisbert
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2023
Subjects:
beluga
physicochemical properties
sevruga
trypsin
digestive physiology
Veterinary medicine
SF600-1100
Zoology
QL1-991
Beluga
Beluga*
Online Access:https://doi.org/10.3390/ani13050853
https://doaj.org/article/e139fd97c31644e6b91a181d2d2a14f8
Description
Summary:This work aimed to determine the physicochemical and biochemical properties of trypsin from beluga Huso huso and sevruga Acipenser stellatus, two highly valuable sturgeon species. According to the results obtained from the methods of casein-zymogram and inhibitory activity staining, the molecular weight of trypsin for sevruga and beluga was 27.5 and 29.5 kDa, respectively. Optimum pH and temperature values for both trypsins were recorded at 8.5 and 55 °C by BAPNA (a specific substrate), respectively. The stability of both trypsins was well-preserved at pH values from 6.0 to 11.0 and temperatures up to 50 °C. TLCK and SBTI, two specific trypsin inhibitors, showed a significant inhibitory effect on the enzymatic activity of both trypsins ( p < 0.05). The enzyme activity was significantly increased in the presence of Ca +2 and surfactants and decreased by oxidizing agents, Cu +2 , Zn +2 , and Co +2 ( p < 0.05). However, univalent ions Na + and K + did not show any significant effect on the activity of both trypsins ( p > 0.05). The results of our study show that the properties of trypsin from beluga and sevruga are in agreement with data reported in bony fish and can contribute to the clear understanding of trypsin activity in these primitive species.

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