Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1)
Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pat...
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ftdoajarticles:oai:doaj.org/article:e12f9eb262f7453aa2b59138cb9802b4 2023-05-15T15:58:19+02:00 Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) M Fuhrmann B Petton V Quillien N Faury B Morga F Pernet 2016-09-01T00:00:00Z https://doi.org/10.3354/aei00197 https://doaj.org/article/e12f9eb262f7453aa2b59138cb9802b4 EN eng Inter-Research https://www.int-res.com/abstracts/aei/v8/p543-552/ https://doaj.org/toc/1869-215X https://doaj.org/toc/1869-7534 1869-215X 1869-7534 doi:10.3354/aei00197 https://doaj.org/article/e12f9eb262f7453aa2b59138cb9802b4 Aquaculture Environment Interactions, Vol 8, Pp 543-552 (2016) Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5 article 2016 ftdoajarticles https://doi.org/10.3354/aei00197 2022-12-31T10:24:53Z Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35‰, 21°C). Water from these tanks was used to infect naïve oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35‰ and (2) oysters acclimated to a salinity of 25‰; the latter were exposed to OsHV-1 water diluted to a salinity of 10 or 25‰. The survival of oysters exposed to OsHV-1 water and acclimated to a salinity of 10‰ was >95%, compared to only 43 to 73% survival in oysters acclimated to higher salinities (Expt 1), reflecting differences in the levels of OsHV-1 DNA and viral gene expression (Expts 1 and 2). However, the survival of their non-acclimated counterparts was only 23% (Expt 2), and the levels of OsHV-1 DNA and the expression of 4 viral genes were low (Expt 1). Thus, OsHV-1 may not have been the ultimate cause of mortality in non-acclimated oysters weakened by a salinity shock. It appears that reducing disease risk by means of low salinity is unlikely in the field. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Pacific Aquaculture Environment Interactions 8 543 552 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5 |
spellingShingle |
Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5 M Fuhrmann B Petton V Quillien N Faury B Morga F Pernet Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
topic_facet |
Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5 |
description |
Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35‰, 21°C). Water from these tanks was used to infect naïve oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35‰ and (2) oysters acclimated to a salinity of 25‰; the latter were exposed to OsHV-1 water diluted to a salinity of 10 or 25‰. The survival of oysters exposed to OsHV-1 water and acclimated to a salinity of 10‰ was >95%, compared to only 43 to 73% survival in oysters acclimated to higher salinities (Expt 1), reflecting differences in the levels of OsHV-1 DNA and viral gene expression (Expts 1 and 2). However, the survival of their non-acclimated counterparts was only 23% (Expt 2), and the levels of OsHV-1 DNA and the expression of 4 viral genes were low (Expt 1). Thus, OsHV-1 may not have been the ultimate cause of mortality in non-acclimated oysters weakened by a salinity shock. It appears that reducing disease risk by means of low salinity is unlikely in the field. |
format |
Article in Journal/Newspaper |
author |
M Fuhrmann B Petton V Quillien N Faury B Morga F Pernet |
author_facet |
M Fuhrmann B Petton V Quillien N Faury B Morga F Pernet |
author_sort |
M Fuhrmann |
title |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_short |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_full |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_fullStr |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_full_unstemmed |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_sort |
salinity influences disease-induced mortality of the oyster crassostrea gigas and infectivity of the ostreid herpesvirus 1 (oshv-1) |
publisher |
Inter-Research |
publishDate |
2016 |
url |
https://doi.org/10.3354/aei00197 https://doaj.org/article/e12f9eb262f7453aa2b59138cb9802b4 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Aquaculture Environment Interactions, Vol 8, Pp 543-552 (2016) |
op_relation |
https://www.int-res.com/abstracts/aei/v8/p543-552/ https://doaj.org/toc/1869-215X https://doaj.org/toc/1869-7534 1869-215X 1869-7534 doi:10.3354/aei00197 https://doaj.org/article/e12f9eb262f7453aa2b59138cb9802b4 |
op_doi |
https://doi.org/10.3354/aei00197 |
container_title |
Aquaculture Environment Interactions |
container_volume |
8 |
container_start_page |
543 |
op_container_end_page |
552 |
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1766394049550352384 |