Seropositivity for Coxiella burnetii in suspected patients with dengue in São Paulo state, Brazil.
Q fever and brucellosis are zoonoses that cause fever and other systemic clinical signs in humans; their occurrences are neglected and the differential diagnosis for some diseases is disregarded. This study aimed to investigate the seropositivity for Coxiella burnetii and Brucella spp. antibodies in...
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ftdoajarticles:oai:doaj.org/article:e0f8503c85b341c19a5246a6efc99fe0 2023-05-15T15:14:50+02:00 Seropositivity for Coxiella burnetii in suspected patients with dengue in São Paulo state, Brazil. Danilo Alves de França Mateus de Souza Ribeiro Mioni Felipe Fornazari Ana Íris de Lima Duré Marcos Vinicius Ferreira Silva Fábio Sossai Possebon Virgínia Bodelão Richini-Pereira Helio Langoni Jane Megid 2022-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010392 https://doaj.org/article/e0f8503c85b341c19a5246a6efc99fe0 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010392 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010392 https://doaj.org/article/e0f8503c85b341c19a5246a6efc99fe0 PLoS Neglected Tropical Diseases, Vol 16, Iss 5, p e0010392 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010392 2022-12-31T02:25:13Z Q fever and brucellosis are zoonoses that cause fever and other systemic clinical signs in humans; their occurrences are neglected and the differential diagnosis for some diseases is disregarded. This study aimed to investigate the seropositivity for Coxiella burnetii and Brucella spp. antibodies in patients suspected of dengue from 38 municipalities in the state of São Paulo, Brazil. The samples (n = 604) were obtained by convenience from the Adolfo Lutz Institute serum bank. Sera were subjected to an indirect immunofluorescence assay (IFA) using in-house and commercial diagnostic protocols to evaluate C. burnetii positivity. For Brucella spp., sera were subjected to rapid plate serum agglutination with buffered acidified antigen (AAT), slow tube serum agglutination (SAL), and 2-mercaptoethanol (2-ME) techniques. Associations and statistical inferences of the results were performed by logistic regression according to the clinical and demographic variables collected from the patients. Statistical analyses were performed using Statistical Analysis Software (SAS) and associations were considered when p value was <0.05. In all, 129 patients showed positive results for Q fever, indicating a seropositivity of 21.4% (95% CI 18.15-24.85). Patients with 14-20 days of symptoms had 2.12 (95% CI 1.34-3.35) times more chances of being seropositive for Q fever than patients with 7-13 days, and patients with 21-27 days of fever had 2.62 (95% CI 1.27-5.41) times more chances of being seropositive for Q fever than patients with 7-13 days. For the other variables analyzed, there were no significant associations between the groups. No positivity for brucellosis was observed. This is the most comprehensive study of people seropositive for Q fever in São Paulo state and provides additional data for the medical community in Brazil. It is suggested that Q fever may be an important differential diagnosis of febrile illnesses in the region, demanding the government's attention and investment in health. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 5 e0010392 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Danilo Alves de França Mateus de Souza Ribeiro Mioni Felipe Fornazari Ana Íris de Lima Duré Marcos Vinicius Ferreira Silva Fábio Sossai Possebon Virgínia Bodelão Richini-Pereira Helio Langoni Jane Megid Seropositivity for Coxiella burnetii in suspected patients with dengue in São Paulo state, Brazil. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Q fever and brucellosis are zoonoses that cause fever and other systemic clinical signs in humans; their occurrences are neglected and the differential diagnosis for some diseases is disregarded. This study aimed to investigate the seropositivity for Coxiella burnetii and Brucella spp. antibodies in patients suspected of dengue from 38 municipalities in the state of São Paulo, Brazil. The samples (n = 604) were obtained by convenience from the Adolfo Lutz Institute serum bank. Sera were subjected to an indirect immunofluorescence assay (IFA) using in-house and commercial diagnostic protocols to evaluate C. burnetii positivity. For Brucella spp., sera were subjected to rapid plate serum agglutination with buffered acidified antigen (AAT), slow tube serum agglutination (SAL), and 2-mercaptoethanol (2-ME) techniques. Associations and statistical inferences of the results were performed by logistic regression according to the clinical and demographic variables collected from the patients. Statistical analyses were performed using Statistical Analysis Software (SAS) and associations were considered when p value was <0.05. In all, 129 patients showed positive results for Q fever, indicating a seropositivity of 21.4% (95% CI 18.15-24.85). Patients with 14-20 days of symptoms had 2.12 (95% CI 1.34-3.35) times more chances of being seropositive for Q fever than patients with 7-13 days, and patients with 21-27 days of fever had 2.62 (95% CI 1.27-5.41) times more chances of being seropositive for Q fever than patients with 7-13 days. For the other variables analyzed, there were no significant associations between the groups. No positivity for brucellosis was observed. This is the most comprehensive study of people seropositive for Q fever in São Paulo state and provides additional data for the medical community in Brazil. It is suggested that Q fever may be an important differential diagnosis of febrile illnesses in the region, demanding the government's attention and investment in health. |
format |
Article in Journal/Newspaper |
author |
Danilo Alves de França Mateus de Souza Ribeiro Mioni Felipe Fornazari Ana Íris de Lima Duré Marcos Vinicius Ferreira Silva Fábio Sossai Possebon Virgínia Bodelão Richini-Pereira Helio Langoni Jane Megid |
author_facet |
Danilo Alves de França Mateus de Souza Ribeiro Mioni Felipe Fornazari Ana Íris de Lima Duré Marcos Vinicius Ferreira Silva Fábio Sossai Possebon Virgínia Bodelão Richini-Pereira Helio Langoni Jane Megid |
author_sort |
Danilo Alves de França |
title |
Seropositivity for Coxiella burnetii in suspected patients with dengue in São Paulo state, Brazil. |
title_short |
Seropositivity for Coxiella burnetii in suspected patients with dengue in São Paulo state, Brazil. |
title_full |
Seropositivity for Coxiella burnetii in suspected patients with dengue in São Paulo state, Brazil. |
title_fullStr |
Seropositivity for Coxiella burnetii in suspected patients with dengue in São Paulo state, Brazil. |
title_full_unstemmed |
Seropositivity for Coxiella burnetii in suspected patients with dengue in São Paulo state, Brazil. |
title_sort |
seropositivity for coxiella burnetii in suspected patients with dengue in são paulo state, brazil. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010392 https://doaj.org/article/e0f8503c85b341c19a5246a6efc99fe0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 5, p e0010392 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010392 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010392 https://doaj.org/article/e0f8503c85b341c19a5246a6efc99fe0 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010392 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
5 |
container_start_page |
e0010392 |
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1766345236558118912 |